1,636 research outputs found
Mrub_1283, Mrub_1284 and Mrub_1285 encode for a glycine/betaine ABC transporter and are orthologs of \u3cem\u3eE. coli\u3c/em\u3e proV, proW and proX
ABC transporters are essential for cellular transport; contribute to maintain the homeostasis of the cells. Generally, ABC transporters are multi-subunit; contain essential cytoplasmic factors which are critical to ATP hydrolysis activity. In this paper, we would like to take a closer look to Mrub_1283, Mrub_1284 and Mrub_1285, three consecutive genes in Meiothermus ruber genome. We hypothesize that these genes are in the same operon and encode for ABC glycine/ betaine transporters. To confirm our hypothesis, we utilizes several bioinformatics tools to predict the potential function of Mrub_1283, Mrub_1284 and Mrub_1285 and to search for their orthologs in Escherichia coli genome. From the data of different bioinformatics tools, Mrub_1283, Mrub_1284 and Mrub_1285 are showed to be orthologous to proV, proW and proX genes in E.coli genomes. As a result, Mrub_1283, Mrub_1284 and Mrub_1285 have the same function with proV, proW and proX therefore encode for ABC glycine/ betaine transporters in Meiothermus ruber
Report on Provider-Client Interaction From 68 Methadone Maintenance Clinics in China.
Provider-client interaction is an integral of clinical practice and central to the delivery of high-quality medical care. This article examines factors related to the provider-client interaction in the context of methadone maintenance treatment (MMT). Data were collected from 68 MMT clinics in China. In total, 418 service providers participated in the survey. Linear mixed effects regression models were performed to identify factors associated with provider-client interaction. It was observed that negative attitude toward drug users was associated with lower level of provider-client interaction and less time spent with each client. Other factors associated with lower level of interaction included being female, being younger, being a nurse, and fewer years in medical field. Higher provider-client interaction was associated with provider reported job satisfaction. The findings of this study call for a need to address provider negative attitudes that can impact provider-client interaction and the effectiveness of MMT. Future intervention efforts targeting MMT providers should be tailored by gender, provider type, and medical experiences
Crystal and solution structures of human oncoprotein Musashiâ2âNâterminal RNA recognition motif 1
This work is licensed under a Creative Commons Attribution 4.0 International License.Musashiâ2 (MSI2) belongs to Musashi family of RNA binding proteins (RBP). Like Musashiâ1 (MSI1), it is overexpressed in a variety of cancers and is a promising therapeutic target. Both MSI proteins contain two Nâterminal RNA recognition motifs and play roles in posttranscriptional regulation of target mRNAs. Previously, we have identified several inhibitors of MSI1, all of which bind to MSI2 as well. In order to design MSI2âspecific inhibitors and compare the differences of binding mode of the inhibitors, we set out to solve the structure of MSI2âRRM1, the key motif that is responsible for the binding. Here, we report the crystal structure and the first NMR solution structure of MSI2âRRM1, and compare these to the structures of MSI1âRBD1 and other RBPs. A high degree of structural similarity was observed between the crystal and solution NMR structures. MSI2âRRM1 shows a highly similar overall folding topology to MSI1âRBD1 and other RBPs. The structural information of MSI2âRRM1 will be helpful for understanding MSI2âRNA interaction and for guiding rational drug design of MSI2âspecific inhibitors
Selective Enrichment and Identification of Azide-tagged Cross-Linked Peptides Using Chemical Ligation and Mass Spectrometry
Protein-protein interaction is one of the key regulatory mechanisms for controlling protein function in various cellular processes. Chemical cross-linking coupled with mass spectrometry has proven to be a powerful method not only for mapping protein-protein interactions of all natures, including weak and transient ones, but also for determining their interaction interfaces. One critical challenge remaining in this approach is how to effectively isolate and identify cross-linked products from a complex peptide mixture. In this work, we have developed a novel strategy using conjugation chemistry for selective enrichment of cross-linked products. An azide-tagged cross-linker along with two biotinylated conjugation reagents were designed and synthesized. Cross-linking of model peptides and cytochrome c as well as enrichment of the resulting cross-linked peptides has been assessed. Selective conjugation of azide-tagged cross-linked peptides has been demonstrated using two strategies: copper catalyzed cycloaddition and Staudinger ligation. While both methods are effective, Staudinger ligation is better suited for enriching the cross-linked peptides since there are fewer issues with sample handling. LC MSn analysis coupled with database searching using the Protein Prospector software package allowed identification of 58 cytochrome c cross-linked peptides after enrichment and affinity purification. The new enrichment strategy developed in this work provides useful tools for facilitating identification of cross-linked peptides in a peptide mixture by MS, thus presenting a step forward in future studies of protein-protein interactions of protein complexes by cross-linking and mass spectrometry
A direct urea fuel cell - power from fertiliser and waste
For the first time, a working direct urea and direct urine fuel cell has been developed to generate electricity directly from urea or urine
E2: a framework for NFV applications
By moving network appliance functionality from proprietary
hardware to software, Network Function Virtualization
promises to bring the advantages of cloud computing to
network packet processing. However, the evolution of cloud
computing (particularly for data analytics) has greatly bene-
fited from application-independent methods for scaling and
placement that achieve high efficiency while relieving programmers
of these burdens. NFV has no such general management
solutions. In this paper, we present a scalable and
application-agnostic scheduling framework for packet processing,
and compare its performance to current approaches
Impact of GnRH agonist triggering and intensive luteal steroid support on live-birth rates and ovarian hyperstimulation syndrome:a retrospective cohort study
BACKGROUND: Conventional luteal support packages are inadequate to facilitate a fresh transfer after GnRH agonist (GnRHa) trigger in patients at high risk of developing ovarian hyperstimulation syndrome (OHSS). By providing intensive luteal-phase support with oestradiol and progesterone satisfactory implantation rates can be sustained. The objective of this study was to assess the live-birth rate and incidence of OHSS after GnRHa trigger and intensive luteal steroid support compared to traditional hCG trigger and conventional luteal support in OHSS high risk Asian patients. METHODS: We conducted a retrospective cohort study of 363 women exposed to GnRHa triggering with intensive luteal support compared with 257 women exposed to conventional hCG triggering. Women at risk of OHSS were defined by ovarian response â„15 follicles â„12Â mm on the day of the trigger. RESULTS: Live-birth rates were similar in both groups GnRHa vs hCG; 29.8% vs 29.2% (pâ=â0.69). One late onset severe OHSS case was observed in the GnRHa trigger group (0.3%) compared to 18 cases (7%) after hCG trigger. CONCLUSIONS: GnRHa trigger combined with intensive luteal steroid support in this group of OHSS high risk Asian patients can facilitate fresh embryo transfer, however, in contrast to previous reports the occurrence of late onset OHSS was not completely eliminated
X-ray and Optical Study of Low Core Density Globular Clusters NGC6144 and E3
We report on the Chandra X-ray Observatory and Hubble Space Telescope
observation of two low core density globular clusters, NGC6144 and E3. By
comparing the number of X-ray sources inside the half-mass radius to those
outside, we found 6 X-ray sources within the half-mass radius of NGC6144, among
which 4 are expected to be background sources; 3 X-ray sources are also found
within the half-mass radius of E3, of which 3 is expected to be background
source. Therefore, we cannot exclude that all our sources are background
sources. However, combining the results from X-ray and optical observations, we
found that 1-2 sources in NGC6144 and 1 source in E3 are likely to be
cataclysmic variables and that 1 source in NGC6144 is an active binary, based
on the X-ray and optical properties. The number of faint X-ray sources in
NGC6144 and E3 found with Chandra and HST is higher than a prediction based on
collision frequency, but is closer to that based on mass. Our observations
strongly suggest that the compact binary systems in NGC6144 and E3 are
primordial in origin.Comment: 28 pages, 9 figures, 6 tables, Accepted for publication in Ap
Nextâgeneration sequencing in precision oncology: Patient understanding and expectations
BackgroundImplementation of precision oncology interventions poses several challenges to informed consent and patient education. This study assessed cancer patientsâ understanding, expectations, and outcomes regarding participation in research examining the impact of matched tumor and germline sequencing on their clinical care.MethodsA total of 297 patients (mean age: 59Â years; 50% female; 96% white) with refractory, metastatic cancer were surveyed, including 217 who completed surveys both before and after undergoing integrated whole exome and transcriptome sequencing as part of a larger clinical research study.ResultsAt baseline, the vast majority of patients expected to receive several potential direct benefits from study participation, including written reports of sequencing findings (88%), greater understanding of the causes of their cancer (74%), and participation in clinical trials for which sequencing results would make them eligible (84%). In most cases, these benefits were not realized by study completion. Despite explanations from study personnel to the contrary, most participants (67%â76%) presumed that incidental germline sequencing findings relevant to noncancerous health conditions (eg, diabetes) would automatically be disclosed to them. Patients reported low levels of concern about study risks at baseline and low levels of regret about study participation at followâup.ConclusionsFindings suggest that cancer patients participating in precision oncology intervention research have largely unfulfilled expectations of direct benefits related to their study participation. Increased focus on patient education to supplement the informed consent process may help manage patientsâ expectations regarding the extent and likelihood of benefits received as a result of undergoing genomic sequencing.This study assessed cancer patientsâ understanding and expectations regarding participation in research examining the impact of matched tumor and germline sequencing on their clinical care. Findings suggest that cancer patients participating in precision oncology intervention research have largely unfulfilled expectations of direct benefits related to their study participation. Increased focus on patient education to supplement the informed consent process may help manage patientsâ expectations regarding the extent and likelihood of benefits received as a result of undergoing genomic sequencing.Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/147745/1/cam41947.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/147745/2/cam41947_am.pd
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