ECONOMIC IMPACTS OF CALIFORNIA'S GOLF COURSE FACILITIES IN 2000

People spent $4.350 billion at California golf course facilities in 2000. The total sales, income, and tax impacts on the state economy were$7.872 billion, $4.546 billion, and$1.370 billion in 2000. Direct sales of $4.251 billion directly supported 62,173 jobs, and , through indirect and induced sales impacts, an additional 37,609 jobs.Land Economics/Use,

Modeling Virus Transport and Removal during Storage and Recovery in Heterogeneous Aquifers

A quantitative understanding of virus removal during aquifer storage and recovery (ASR) in physically and geochemically heterogeneous aquifers is needed to accurately assess human health risks from viral infections. A two-dimensional axisymmetric numerical model incorporating processes of virus attachment, detachment, and inactivation in aqueous and solid phases was developed to systematically evaluate the virus removal performance of ASR schemes. Physical heterogeneity was considered as either layered or randomly distributed hydraulic conductivities (with selected variance and horizontal correlation length). Geochemical heterogeneity in the aquifer was accounted for using Colloid Filtration Theory to predict the spatial distribution of attachment rate coefficient. Simulation results demonstrate that the combined effects of aquifer physical heterogeneity and spatial variability of attachment rate resulted in higher virus concentrations in the recovered water at the ASR well (i.e. reduced virus removal). While the sticking efficiency of viruses to aquifer sediments was found to significantly influence virus concentration in the recovered water, the solid phase inactivation under realistic field conditions combined with the duration of storage phase had a predominant influence on the overall virus removal. The relative importance of physical heterogeneity increased under physicochemical conditions that reduced virus removal (e.g. lower value of sticking efficiency or solid phase inactivation rate). This study provides valuable insight on site selection of ASR projects and an approach to optimize ASR operational parameters (e.g. storage time) for virus removal and to minimize costs associated with post-recovery treatment

Development and validation of a chemostat gut model to study both planktonic and biofilm modes of growth of Clostridium difficile and human microbiota

Copyright: 2014 Crowther et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.The human gastrointestinal tract harbours a complex microbial community which exist in planktonic and sessile form. The degree to which composition and function of faecal and mucosal microbiota differ remains unclear. We describe the development and characterisation of an in vitro human gut model, which can be used to facilitate the formation and longitudinal analysis of mature mixed species biofilms. This enables the investigation of the role of biofilms in Clostridium difficile infection (CDI). A well established and validated human gut model of simulated CDI was adapted to incorporate glass rods that create a solid-gaseous-liquid interface for biofilm formation. The continuous chemostat model was inoculated with a pooled human faecal emulsion and controlled to mimic colonic conditions in vivo. Planktonic and sessile bacterial populations were enumerated for up to 46 days. Biofilm consistently formed macroscopic structures on all glass rods over extended periods of time, providing a framework to sample and analyse biofilm structures independently. Whilst variation in biofilm biomass is evident between rods, populations of sessile bacterial groups (log10 cfu/g of biofilm) remain relatively consistent between rods at each sampling point. All bacterial groups enumerated within the planktonic communities were also present within biofilm structures. The planktonic mode of growth of C. difficile and gut microbiota closely reflected observations within the original gut model. However, distinct differences were observed in the behaviour of sessile and planktonic C. difficile populations, with C. difficile spores preferentially persisting within biofilm structures. The redesigned biofilm chemostat model has been validated for reproducible and consistent formation of mixed species intestinal biofilms. This model can be utilised for the analysis of sessile mixed species communities longitudinally, potentially providing information of the role of biofilms in CDI.Peer reviewe

Suppression of electron spin decoherence of the diamond NV center by a transverse magnetic field

We demonstrate that the spin decoherence of nitrogen vacancy (NV) centers in diamond can be suppressed by a transverse magnetic field if the electron spin bath is the primary decoherence source. The NV spin coherence, created in "a decoherence-free subspace" is protected by the transverse component of the zero-field splitting, increasing the spin-coherence time about twofold. The decoherence due to the electron spin bath is also suppressed at magnetic fields stronger than ~25 gauss when applied parallel to the NV symmetry axis. Our method can be used to extend the spin-coherence time of similar spin systems for applications in quantum computing, field sensing, and other metrologies.Comment: 20 pages, 4 figure