The value of twins in dental research

The document attached has been archived with permission from the Australian Dental Association. An external link to the publisher’s copy is included.Due to recent advances in molecular genetics, studies of twins will continue to provide important insights into how genetic and environmental factors contribute to variation in human physical and behavioural traits and disorders. This review emphasizes that biometrical genetic studies of twins are particularly valuable in complementing and directing molecular approaches to facilitate the detection of quantitative trait loci. It also describes several other research models involving twins, apart from the traditional comparison of similarities in monozygotic (identical) and dizygotic (nonidentical) pairs, that have the potential to provide new information in the future. Current knowledge about the genetic bases of common dental problems is summarized and future directions in dental research involving twins are outlined.GC Townsend, L Richards, T Hughes, S Pinkerton, W Schwerd

Case studies to enhance graduate employability: Indigenous employment and supports

This is one in a series of case studies to enhance graduate employability. This case study on the context of Indigenous employment and supports is based on interviews and focus groups with 14 people across the stakeholder groups of employed graduates, students in internships, educators,career development professionals and employers from six different universities

Mygalomorphae spider community of the Natural Reserve Sierra del Tigre, Tandilia, Buenos Aires, Argentina

We documented the specific richness, abundance and activity of the Mygalomorph spider community in the Natural Reserve Sierra del Tigre, Buenos Aires, Argentina. This hilly ecosystem is home for many endemic species and rich in native fauna and flora. Spider abundance was sampled monthly from May 2011 - May 2012 by hand capture and pitfall traps. The species recorded in the study area were Actinopus sp. 1 (Actinopodidae), Grammostola vachoni Schiapelli y Gerschman, 1960; Plesiopelma longisternale Schiapelli y Gerschman, 1942 and Catumiri argentinense (Mello-Leitão, 1941) (Theraphosidae). Grammostola vachoni was the dominant species in hand capture and Actinopus sp. 1 in pitfall traps. The seasonal variation, diversity, and abundance of the mygalomorph community are analyzed and discussed here. The Mygalomorphae of the Tandilia system comprises an important group of sedentary and cryptozoic spiders that seem to be dependent on habitat type and environmental factors.En el presente trabajo se analizó la riqueza específica, abundancia y actividad de la comunidad de arañas Mygalomorphae en la Reserva Natural Sierra del Tigre, Buenos Aires, Argentina. Este ecosistema serrano alberga numerosas especies endémicas y es rico en fauna y flora nativa. La abundancia de arañas se muestreó mensualmente desde mayo de 2011 a mayo de 2012, mediante recolección manual y trampas de caída. Las especies registradas en el área de estudio fueron Actinopus sp. 1 (Actinopodidae), Grammostola vachoni Schiapelli y Gerschman, 1960; Plesiopelma longisternale Schiapelli y Gerschman, 1942 y Catumiri argentinense (Mello-Leitão, 1941) (Theraphosidae). Grammostola vachoni resultó la especie dominante en la recolección manual y Actinopus sp. 1 en trampas de caída. Se discuten y analizan la variación estacional, diversidad y abundancia de la comunidad de migalomorfas. Las Mygalomorphae del sistema de Tandilia comprenden un grupo importante de arañas sedentarias y criptozoicas que parecen depender del tipo de ambiente y factores ambientales.Centro de Estudios Parasitológicos y de Vectore

Mygalomorphae spider community of the Natural Reserve Sierra del Tigre, Tandilia, Buenos Aires, Argentina

We documented the specific richness, abundance and activity of the Mygalomorph spider community in the Natural Reserve Sierra del Tigre, Buenos Aires, Argentina. This hilly ecosystem is home for many endemic species and rich in native fauna and flora. Spider abundance was sampled monthly from May 2011 - May 2012 by hand capture and pitfall traps. The species recorded in the study area were Actinopus sp. 1 (Actinopodidae), Grammostola vachoni Schiapelli y Gerschman, 1960; Plesiopelma longisternale Schiapelli y Gerschman, 1942 and Catumiri argentinense (Mello-Leitão, 1941) (Theraphosidae). Grammostola vachoni was the dominant species in hand capture and Actinopus sp. 1 in pitfall traps. The seasonal variation, diversity, and abundance of the mygalomorph community are analyzed and discussed here. The Mygalomorphae of the Tandilia system comprises an important group of sedentary and cryptozoic spiders that seem to be dependent on habitat type and environmental factors.En el presente trabajo se analizó la riqueza específica, abundancia y actividad de la comunidad de arañas Mygalomorphae en la Reserva Natural Sierra del Tigre, Buenos Aires, Argentina. Este ecosistema serrano alberga numerosas especies endémicas y es rico en fauna y flora nativa. La abundancia de arañas se muestreó mensualmente desde mayo de 2011 a mayo de 2012, mediante recolección manual y trampas de caída. Las especies registradas en el área de estudio fueron Actinopus sp. 1 (Actinopodidae), Grammostola vachoni Schiapelli y Gerschman, 1960; Plesiopelma longisternale Schiapelli y Gerschman, 1942 y Catumiri argentinense (Mello-Leitão, 1941) (Theraphosidae). Grammostola vachoni resultó la especie dominante en la recolección manual y Actinopus sp. 1 en trampas de caída. Se discuten y analizan la variación estacional, diversidad y abundancia de la comunidad de migalomorfas. Las Mygalomorphae del sistema de Tandilia comprenden un grupo importante de arañas sedentarias y criptozoicas que parecen depender del tipo de ambiente y factores ambientales.Centro de Estudios Parasitológicos y de Vectore

Genetics and physiology of cell wall polysaccharides in the model C(4) grass, Setaria viridis spp

Published: 2 October 2015BACKGROUND: Setaria viridis has emerged as a model species for the larger C4 grasses. Here the cellulose synthase (CesA) superfamily has been defined, with an emphasis on the amounts and distribution of (1,3;1,4)-β-glucan, a cell wall polysaccharide that is characteristic of the grasses and is of considerable value for human health. METHODS: Orthologous relationship of the CesA and Poales-specific cellulose synthase-like (Csl) genes among Setaria italica (Si), Sorghum bicolor (Sb), Oryza sativa (Os), Brachypodium distachyon (Bradi) and Hordeum vulgare (Hv) were compared using bioinformatics analysis. Transcription profiling of Csl gene families, which are involved in (1,3;1,4)-β-glucan synthesis, was performed using real-time quantitative PCR (Q-PCR). The amount of (1,3;1,4)-β-glucan was measured using a modified Megazyme assay. The fine structures of the (1,3;1,4)-β-glucan, as denoted by the ratio of cellotriosyl to cellotetraosyl residues (DP3:DP4 ratio) was assessed by chromatography (HPLC and HPAEC-PAD). The distribution and deposition of the MLG was examined using the specific antibody BG-1 and captured using fluorescence and transmission electron microscopy (TEM). RESULTS: The cellulose synthase gene superfamily contains 13 CesA and 35 Csl genes in Setaria. Transcript profiling of CslF, CslH and CslJ gene families across a vegetative tissue series indicated that SvCslF6 transcripts were the most abundant relative to all other Csl transcripts. The amounts of (1,3;1,4)-β-glucan in Setaria vegetative tissues ranged from 0.2% to 2.9% w/w with much smaller amounts in developing grain (0.003% to 0.013% w/w). In general, the amount of (1,3;1,4)-β-glucan was greater in younger than in older tissues. The DP3:DP4 ratios varied between tissue types and across developmental stages, and ranged from 2.4 to 3.0:1. The DP3:DP4 ratios in developing grain ranged from 2.5 to 2.8:1. Micrographs revealing the distribution of (1,3;1,4)-β-glucan in walls of different cell types and the data were consistent with the quantitative (1,3;1,4)-β-glucan assays. CONCLUSION: The characteristics of the cellulose synthase gene superfamily and the accumulation and distribution of (1,3;1,4)-β-glucans in Setaria are similar to those in other C4 grasses, including sorghum. This suggests that Setaria is a suitable model plant for cell wall polysaccharide biology in C4 grasses.Riksfardini A. Ermawar, Helen M. Collins, Caitlin S. Byrt, Marilyn Henderson, Lisa A. O'Donovan, Neil J. Shirley, Julian G. Schwerdt, Jelle Lahnstein, Geoffrey B. Fincher and Rachel A. Burto

A chromosome-level genome assembly of Plantago ovata

Plantago ovata is cultivated for production of its seed husk (psyllium). When wet, the husk transforms into a mucilage with properties suitable for pharmaceutical industries, utilised in supplements for controlling blood cholesterol levels, and food industries for making gluten-free products. There has been limited success in improving husk quantity and quality through breeding approaches, partly due to the lack of a reference genome. Here we constructed the frst chromosome-scale reference assembly of P. ovata using a combination of 5.98 million PacBio and 636.5 million Hi-C reads. We also used corrected PacBio reads to estimate genome size and transcripts to generate gene models. The fnal assembly covers ~ 500 Mb with 99.3% gene set completeness. A total of 97% of the sequences are anchored to four chromosomes with an N50 of~ 128.87 Mb. The P. ovata genome contains 61.90% repeats, where 40.04% are long terminal repeats. We identifed 41,820 protein-coding genes, 411 non-coding RNAs, 108 ribosomal RNAs, and 1295 transfer RNAs. This genome will provide a resource for plant breeding programs to, for example, reduce agronomic constraints such as seed shattering, increase psyllium yield and quality, and overcome crop disease susceptibility.Lina Herliana, Julian G. Schwerdt, Tycho R. Neumann, Anita Severn-Ellis, Jana L. Phan, James M. Cowley, Neil J. Shirley, Matthew R.Tucker, Tina Bianco, Miotto, Jacqueline Batley, Nathan S. Watson, Haigh, Rachel A. Burto

A Rapid and Simple Procedure for the Establishment of Human Normal and Cancer Renal Primary Cell Cultures from Surgical Specimens

The kidney is a target organ for the toxicity of several xenobiotics and is also highly susceptible to the development of malignant tumors. In both cases, in vitro studies provide insight to cellular damage, and represent adequate models to study either the mechanisms underlying the toxic effects of several nephrotoxicants or therapeutic approaches in renal cancer. The development of efficient methods for the establishment of human normal and tumor renal cell models is hence crucial. In this study, a technically simple and rapid protocol for the isolation and culture of human proximal tubular epithelial cells and human renal tumor cells from surgical specimens is presented. Tumor and normal tissues were processed by using the same methodology, based on mechanical disaggregation of tissue followed by enzymatic digestion and cell purification by sequential sieving. The overall procedure takes roughly one hour. The resulting cell preparations have excellent viabilities and yield. Establishment of primary cultures from all specimens was achieved successfully. The origin of primary cultured cells was established through morphological evaluation. Normal cells purity was confirmed by immunofluorescent staining and reverse transcription-polymerase chain reaction analysis for expression of specific markers

Plant-made polio type 3 stabilized VLPs—a candidate synthetic polio vaccine

Poliovirus (PV) is the causative agent of poliomyelitis, a crippling human disease known since antiquity. PV occurs in two distinct antigenic forms, D and C, of which only the D form elicits a robust neutralizing response. Developing a synthetically produced stabilized viruslike particle (sVLP)-based vaccine with D antigenicity, without the drawbacks of current vaccines, will be a major step towards the final eradication of poliovirus. Such a sVLP would retain the native antigenic conformation and the repetitive structure of the original virus particle, but lack infectious genomic material. In this study, we report the production of synthetically stabilized PV VLPs in plants. Mice carrying the gene for the human PV receptor are protected from wild-type PV when immunized with the plant-made PV sVLPs. Structural analysis of the stabilized mutant at 3.6 Å resolution by cryo-electron microscopy and single particle reconstruction reveals a structure almost indistinguishable from wild-type PV3