Optical vortex coronagraphs on ground-based telescopes

The optical vortex coronagraph is potentially a remarkably effective device, at least for an ideal unobstructed telescope. Most ground-based telescopes however suffer from central obscuration and also have to operate through the aberrations of the turbulent atmosphere. This note analyzes the performance of the optical vortex in these circumstances and compares to some other designs, showing that it performs similarly in this situation. There is a large class of coronagraphs of this general type, and choosing between them in particular applications depends on details of performance at small off-axis distances and uniformity of response in the focal plane. Issues of manufacturability to the necessary tolerances are also likely to be important.Comment: 32 pages, 13 figure

Thermodynamic metrics and optimal paths

A fundamental problem in modern thermodynamics is how a molecular-scale machine performs useful work, while operating away from thermal equilibrium without excessive dissipation. To this end, we derive a friction tensor that induces a Riemannian manifold on the space of thermodynamic states. Within the linear-response regime, this metric structure controls the dissipation of finite-time transformations, and bestows optimal protocols with many useful properties. We discuss the connection to the existing thermodynamic length formalism, and demonstrate the utility of this metric by solving for optimal control parameter protocols in a simple nonequilibrium model.Comment: 5 page

Biology and Biological Control of Exotic True Thistles

“Thistle” is an old English name for a large variety of weedy, prickly plants that grow throughout the world. The most notable characteristics of thistles are the prickly stems and leaves and the bracts around the flower head. While many different plants have “thistle” in their common name, only certain plant species fit the taxonomic requirements of being considered “true thistles.” True thistle species fall within the family Asteraceae, the tribe Cardueae, and the subtribe Carduinae. Examples of plants that are not true thistles include yellow starthistle (subtribe Centaureinae), sow thistle (subtribe Sonchinae), and Russian thistle (family Chenopodiaceae). Only true thistles in the subtribe Carduinae are discussed in this manual

Biology and Biological Control of Exotic True Thistles

“Thistle” is an old English name for a large variety of weedy, prickly plants that grow throughout the world. The most notable characteristics of thistles are the prickly stems and leaves and the bracts around the flower head. While many different plants have “thistle” in their common name, only certain plant species fit the taxonomic requirements of being considered “true thistles.” True thistle species fall within the family Asteraceae, the tribe Cardueae, and the subtribe Carduinae. Examples of plants that are not true thistles include yellow starthistle (subtribe Centaureinae), sow thistle (subtribe Sonchinae), and Russian thistle (family Chenopodiaceae). Only true thistles in the subtribe Carduinae are discussed in this manual

Advanced Communication Theory Techniques TECHNICAL DOCUMENTARY REPORT NO. ASD-TDR-63-186

Under this contract a number of topics have been studied and analyzed in detail in order to bring together and somewhat extend the concepts of communication theory as they apply to some current problems in digital communication systems. Radio wave channels are characterized by a model\u27 which accounts for both multiplicative and additive disturbances, A large amount of experimental data pertaining to radio disturbances is evaluated and correlated. She. importance of the Rayleigh fading channel is emphasized and previous work is extended to determine the capacity and efficiency of the Rayleigh, channel. Detection theory concepts have been extended to treat the problem of signal detection in the presence of statistically unknown additive disturbances. Several detectors based on non-parametric statistical techniques are treated in detail. Obese detectors are compared to the conventional likelihood detectors. Design procedures are formulated. Signal design techniques are used to optimize transmitted wave- forms and the improvement in system performance is determined. The criterion used in this\u27 analysis is the minimization of intersymbol influence and the minimization of transmitter power for a fixed probability of received, errors . The tradeoffs available between transmitter power and coding complexity are thoroughly investigated for the binary symmetric channel. Results are obtained for both Hamming and Bose-Chandhuri codes. Recommendations for further work in promising areas are made, the need to supplement theoretical work with experimental work is pointed ou

Chloroplasts lacking class I glutaredoxins are functional but show a delayed recovery of protein cysteinyl redox state after oxidative challenge

Redox status of protein cysteinyl residues is mediated via glutathione (GSH)/glutaredoxin (GRX) and thioredoxin (TRX)-dependent redox cascades. An oxidative challenge can induce post-translational protein modifications on thiols, such as protein S-glutathionylation. Class I GRX are small thiol-disulfide oxidoreductases that reversibly catalyse S-glutathionylation and protein disulfide formation. TRX and GSH/GRX redox systems can provide partial backup for each other in several subcellular compartments, but not in the plastid stroma where TRX/light-dependent redox regulation of primary metabolism takes place. While the stromal TRX system has been studied at detail, the role of class I GRX on plastid redox processes is still unknown. We generate knockout lines of GRXC5 as the only chloroplast class I GRX of the moss Physcomitrium patens. While we find that PpGRXC5 has high activities in GSH-dependent oxidoreductase assays using hydroxyethyl disulfide or redox-sensitive GFP2 as substrates in vitro, Δgrxc5 plants show no detectable growth defect or stress sensitivity, in contrast to mutants with a less negative stromal EGSH (Δgr1). Using stroma-targeted roGFP2, we show increased protein Cys steady state oxidation and decreased reduction rates after oxidative challenge in Δgrxc5 plants in vivo, indicating kinetic uncoupling of the protein Cys redox state from EGSH. Compared to wildtype, protein Cys disulfide formation rates and S-glutathionylation levels after H2O2 treatment remained unchanged. Lack of class I GRX function in the stroma did not result in impaired carbon fixation. Our observations suggest specific roles for GRXC5 in the efficient transfer of electrons from GSH to target protein Cys as well as negligible cross-talk with metabolic regulation via the TRX system. We propose a model for stromal class I GRX function in efficient catalysis of protein dithiol/disulfide equilibria upon redox steady state alterations affecting stromal EGSH and highlight the importance of identifying in vivo target proteins of GRXC5

Selective Ion Changes during Spontaneous Mitochondrial Transients in Intact Astrocytes

The bioenergetic status of cells is tightly regulated by the activity of cytosolic enzymes and mitochondrial ATP production. To adapt their metabolism to cellular energy needs, mitochondria have been shown to exhibit changes in their ionic composition as the result of changes in cytosolic ion concentrations. Individual mitochondria also exhibit spontaneous changes in their electrical potential without altering those of neighboring mitochondria. We recently reported that individual mitochondria of intact astrocytes exhibit spontaneous transient increases in their Na+ concentration. Here, we investigated whether the concentration of other ionic species were involved during mitochondrial transients. By combining fluorescence imaging methods, we performed a multiparameter study of spontaneous mitochondrial transients in intact resting astrocytes. We show that mitochondria exhibit coincident changes in their Na+ concentration, electrical potential, matrix pH and mitochondrial reactive oxygen species production during a mitochondrial transient without involving detectable changes in their Ca2+ concentration. Using widefield and total internal reflection fluorescence imaging, we found evidence for localized transient decreases in the free Mg2+ concentration accompanying mitochondrial Na+ spikes that could indicate an associated local and transient enrichment in the ATP concentration. Therefore, we propose a sequential model for mitochondrial transients involving a localized ATP microdomain that triggers a Na+-mediated mitochondrial depolarization, transiently enhancing the activity of the mitochondrial respiratory chain. Our work provides a model describing ionic changes that could support a bidirectional cytosol-to-mitochondria ionic communication

Sodium signaling and astrocyte energy metabolism.

The Na(+) gradient across the plasma membrane is constantly exploited by astrocytes as a secondary energy source to regulate the intracellular and extracellular milieu, and discard waste products. One of the most prominent roles of astrocytes in the brain is the Na(+) -dependent clearance of glutamate released by neurons during synaptic transmission. The intracellular Na(+) load collectively generated by these processes converges at the Na,K-ATPase pump, responsible for Na(+) extrusion from the cell, which is achieved at the expense of cellular ATP. These processes represent pivotal mechanisms enabling astrocytes to increase the local availability of metabolic substrates in response to neuronal activity. This review presents basic principles linking the intracellular handling of Na(+) following activity-related transmembrane fluxes in astrocytes and the energy metabolic pathways involved. We propose a role of Na(+) as an energy currency and as a mediator of metabolic signals in the context of neuron-glia interactions. We further discuss the possible impact of the astrocytic syncytium for the distribution and coordination of the metabolic response, and the compartmentation of these processes in cellular microdomains and subcellular organelles. Finally, we illustrate future avenues of investigation into signaling mechanisms aimed at bridging the gap between Na(+) and the metabolic machinery. GLIA 2016;64:1667-1676