Befragung der Patienten zur Qualität der Krankenhausverpflegung und zu ihrer Einstellung zu Biokost sowie der Kliniken zum Einsatz von Biokost in ihrer Speiseversorgung

Um die Meinung von Krankenhauspatienten zur Qualität ihrer Verpflegung und zu ihrer Einstellung zu Bio-Lebensmitteln zu ermitteln, wurde vom Freiburger Institut für Umweltmedizin und Krankenhausyhgiene eine Umfrage durchgeführt (gefördert durch das Bundesprogramm Ökologischer Landbau). An der Umfrage beteiligten sich 1.500 Patientinnen und Patienten von 25 Akut- und Rehakliniken aus der Umgebung des Universitätsklinikums Freiburg. Auch die Verwaltungen konnten Ihre Meinung äußern. Im Ergebnis sind die befragten Patienten mit ihrer Essensqualität zufrieden, sie würden aber biologisch erzeugte Lebensmittel durchaus begrüßen, insbesondere da sie diese für gesünder halten als konventionell erzeugte Ware. Auch bezüglich Geschmack, Qualität und Umweltfreundlichkeit wurde die Biokost ausgesprochen positiv bewertet. Von Patienten bevorzugte Bio-Komponenten sind Gemüse, Obst und Salat. Generell wird auf eine gute Krankenverpflegung von Seiten der Patienten ein sehr hoher Wert gelegt. Weitgehende Zustimmung fand auch die Aussage, dass die Ernährungsqualität mit Einfluss auf den Behandlungserfolg hat. Allerdings stehen die Klinikverwaltungen finanziell sehr unter Druck. Der durchschnittliche Lebensmitteleinsatz für eine komplette Patienten-Tagesverpflegung lag bei 4,90 Euro und für Mehrausgaben wird von Seiten der Küchen kaum Spielraum gesehen. Die Umfrage zeigt aber, dass Kliniken, die in ihrer Küche trotzdem eine konsequente Strategie für eine sehr gute Verpflegungsqualität, einschließlich der Verwendung von Lebensmitteln in Bioqualität, verfolgen, von ihren Patienten mit einer hohen Zufriedenheit messbar honoriert werden

Software-in-the-Loop Simulation of a Planetary Rover

The development of autonomous navigation algorithms for planetary rovers often hinges on access to rover hardware. Yet this access is usually very limited. In order to facilitate the continued development of these algorithms even when the hardware is temporarily unavailable, simulations are used. To minimize any additional work, these simulations must tightly integrate with the rover’s software infrastructure. They are then called Software-in-the-Loop simulators. In preparation for the 2015 DLR SpaceBot Camp, a simulation of the DLR LRU rover became necessary to ensure a timely progress of the navigation algorithms development. This paper presents the Software-in-the-loop simulator of the LRU, including details on the implementation and application

Signatures of electronic polarons in La1−x_{1-x}Sr1+x_{1+x}MnO4_4 observed by electron energy-loss spectroscopy

The dielectric properties of La$_{1-x}$Sr$_{1+x}$MnO$_4$ single crystals with x = 0, 0.125, 0.25, and 0.5 were studied by means of electron energy-loss spectroscopy as a function of temperature and momentum transfer. A clear signature of the doped holes is observed around 1.65 eV energy loss, where spectral weight emerges with increasing x. For all $x \neq 0$, this doping-induced excitation can propagate within the ab-plane, as revealed by a clear upward dispersion of the corresponding loss peak with increasing momentum transfer. The hole-induced excitation also shifts to higher energies with the onset of magnetic correlations for x = 0.5, implying a strong coupling of charge and spin dynamics. We conclude that (i) the loss feature at 1.65 eV is a signature of electronic polarons, which are created around doped holes, and that (ii) this low-energy excitation involves the charge transfer between manganese and oxygen. The finite dispersion of these excitations further indicates significant polaron-polaron interactions.Comment: 7 pages, 4 figure

Novel method for high throughput DNA methylation marker evaluation using PNA-probe library hybridization and MALDI-TOF detection

The methylation of CpG dinucleotides has become a topic of great interest in cancer research, and the methylation of promoter regions of several tumor suppressor genes has been identified as a marker of tumorigenesis. Evaluation of DNA methylation markers in tumor tissue requires hundreds of samples, which must be analyzed quantitatively due to the heterogeneous composition of biological material. Therefore novel, fast and inexpensive methods for high throughput analysis are needed. Here we introduce a new assay based on peptide nucleic acid (PNA)-library hybridization and subsequent MALDI-TOF analysis. This method is multiplexable, allows the use of standard 384 well automated pipetting, and is more specific and flexible than established methods, such as microarrays and MS-SNuPE. The approach was used to evaluate three candidate colon cancer methylation markers previously identified in a microarray study. The methylation of the genes Ade-nomatous polyposis coli (APC), glycogen synthase kinase-β-3 (GSK3β) and eyes absent 4 (EYA4) was analyzed in 12 colon cancer and 12 normal tissues. APC and EYA4 were confirmed as being differentially methylated in colon cancer patients whereas GSK3β did not show differential methylation

The Quaternary of the southwest German Alpine Foreland (Bodensee-Oberschwaben, Baden-Württemberg, Southwest Germany)

Das Quartär der Bodensee-Region besteht aus Schottern frühpleistozäner alpiner Flusssysteme (Deckenschotter) sowie aus glazialen und Schmelzwasser-Ablagerungen der mittel- und spätpleistozänen Eiszeiten. Sie belegen den landschaftlichen Wandel von einer Art Rampe aus Vorbergen hin zur heutigen Topographie mit ineinander greifenden, übertieften Becken, sodass sich eine Art Amphitheater ergibt. Die Deckenschotter als älteste Ablagerungen dokumentieren einerseits die Eintiefung der alpinen Flüsse in diversen Terrassenstufen im Sedimentationsgebiet, andererseits durch deutliche Unterschiede im Geröllspektrum die Vergrößerung des Liefergebiets des sich entwickelnden alpinen Rheins. Der älteste Till kommt vor in Kontakt mit Mindel-Deckenschottern, es gibt jedoch keine Hinweise auf eine glaziale Übertiefung in dieser Zeit. Die meisten glazialen und Schmelzwasser-Ablagerungen werden drei großen Vergletscherungen des Rheingletschers zugeordnet. Diese Vorlandvergletscherungen sind mit drei Generationen glazialer Becken verknüpft. Die ältesten Becken sind zur Donau orientiert, die aus der letzten Vereisung entwässern zum Rhein. Diese Reorientierung bewirkte die hervorragende räumliche Auflösung der Sedimente und Formen. Traditionell wurden die Sedimente in einem chronostratigraphischen System aus glazialen und interglazialen Stufen beschrieben. Unsere Ziele in dieser Arbeit sind, eine Aktualisierung des chronostratigraphischen Systems vorzustellen, das neue, beim geologischen Dienst von Baden-Württemberg angewandte, lithostratigraphische Schema zu erklären und die wichtigsten neuen Einheiten kurz zu beschreiben.researc

Early molecular insights into thanatin analogues binding to A. baumannii LptA

The cationic antimicrobial ß-hairpin, thanatin, was recently developed into drug-like analogues active against carbapenem-resistant Enterobacteriaceae (CRE). The analogues represent new antibiotics with a novel mode of action targeting LptA in the periplasm and disrupting LPS transport. The compounds lose antimicrobial efficacy when the sequence identity to E. coli LptA falls below 70%. We wanted to test the thanatin analogues against LptA of a phylogenetic distant organism and investigate the molecular determinants of inactivity. Acinetobacter baumannii (A. baumannii) is a critical Gram-negative pathogen that has gained increasing attention for its multi-drug resistance and hospital burden. A. baumannii LptA shares 28% sequence identity with E. coli LptA and displays an intrinsic resistance to thanatin and thanatin analogues (MIC values > 32 μg/mL) through a mechanism not yet described. We investigated the inactivity further and discovered that these CRE- optimized derivatives can bind to LptA of A. baumannii in vitro, despite the high MIC values. Herein, we present a high-resolution structure of A. baumannii LptAm in complex with a thanatin derivative 7 and binding affinities of selected thanatin derivatives. Together, these data offer structural insights into why thanatin derivatives are inactive against A. baumannii LptA, despite binding events in vitro

MALDI mass spectrometry imaging workflow for the aquatic model organisms Danio rerio and Daphnia magna

Lipids play various essential roles in the physiology of animals. They are also highly dependent on cellular metabolism or status. It is therefore crucial to understand to which extent animals can stabilize their lipid composition in the presence of external stressors, such as chemicals that are released into the environment. We developed a MALDI MS imaging workflow for two important aquatic model organisms, the zebrafish (Danio rerio) and water flea (Daphnia magna). Owing to the heterogeneous structure of these organisms, developing a suitable sample preparation workflow is a highly non-trivial but crucial part of this work and needs to be established first. Relevant parameters and practical considerations in order to preserve tissue structure and composition in tissue sections are discussed for each application. All measurements were based on high mass accuracy enabling reliable identification of imaged compounds. In zebrafish we demonstrate that a detailed mapping between histology and simultaneously determined lipid composition is possible at various scales, from extended structures such as the brain or gills down to subcellular structures such as a single axon in the central nervous system. For D. magna we present for the first time a MALDI MSI workflow, that demonstrably maintains tissue integrity during cryosectioning of non-preserved samples, and allows the mapping of lipids in the entire body and the brood chamber inside the carapace. In conclusion, the lipid signatures that we were able to detect with our method provide an ideal basis to analyze changes caused by pollutants in two key aquatic model organisms

Optimization approach for the combined planning and control of an agile assembly system for electric vehicles

For some years now, the automotive industry has been challenged by growing market dynamics, shorter product lifecycles and customers' increasing demands for individualization. In order to cope with this development, the automotive assembly needs to adapt quickly to changing demands with a low level of investment in the future. Under the current circumstances, the traditional line assembly for high volume production is reaching its limits in terms of adaptability and scalability. A promising solution to address the current challenges is the concept of the agile assembly. The concept of agile assembly breaks up the rigid linkage of assembly stations and, thus, enables full flexibility in the sequence of assembly operations only limited by the precedence graph. Therefore, the routing of electric vehicles in the agile assembly is based on the availability of resources such as assembly stations and automated guided vehicles that handle the material supply. Further, by transferring the transport function to the vehicle itself, investments for convey or systems are eliminated. This research work presents an optimization approach for the machine scheduling and transportation planning, which derives instructions for electric vehicles, assembly stations as well as automated guided vehicles. For each electric vehicle, an optimized route is calculated, taking into account product-specific precedence graphs and minimizing the overall makespan. In addition, the machine scheduling and transportation planning is integrated into a combined planning and control concept which covers the allocation of resources and the assignment of capabilities of the entire assembly system. The approach is implemented and applied to a practical case of a compact electric vehicle. Thus, the work contributes to the evaluation of agile assembly systems in automotive production

Peptides in BioNMR Research

Heteronuclear NMR in combination with isotope labelling is used to study folding of polypeptides induced by metals in the case of metallothioneins, binding of the peptidic allosteric modulator ?-TIA to the human G-protein coupled ?1b adrenergic receptor, the development of therapeutic drugs that interfere with the biosynthesis of the outer membrane of Gram-negative bacteria, and a system in which protein assembly is induced upon peptide addition. NMR in these cases is used to derive precise structural data and to study the dynamics