Magnetic Response Versus Lift Height of Thin Ferromagnetic Films

The interaction between a magnetic force microscope (MFM) tip and ferromagnetic films of Ni, Co90Fe10 and Py with in-plane magnetization has been investigated. The measured interaction, due to the magnetizing of the films by the MFM tip field, was determined by the phase shift of the cantilever response. The tip-film separation or lift height dependent phase shift was found to be independent of the saturation magnetization of the ferromagnetic film. The result is identical for all three films and micromagnetic simulations give similar results. The reason is at a given tip-sample separation the tip induced magnetization of the film creates a demagnetization field which is equal in magnitude to the tip field at that separation

The usage of mesenchymal stem cells in the treatment of type 1 diabetes mellitus

Type 1 diabetes mellitus is a metabolic disorder characterized by an autoimmune attack against the insulin producing Beta-cells of the pancreas. Also known as insulin-dependent diabetes, patients must receive exogenous injections of insulin in order to maintain glycemic homeostasis. The necessity of monitoring one’s own blood glucose levels and self-administering insulin is a tedious routine for type 1 diabetics, and this standard treatment option fails to treat any of the underlying causes of the disease. According to van Belle et al, the prevalence of diabetes is rising worldwide amongst all age-groups, from 2.8% in 2000 to an estimated 4.4% by 2030, thus the need to find a more curative treatment approach is eminent. In the emerging field of regenerative medicine, mesenchymal stem cells have been identified as a possible therapeutic tool to replace damaged parenchymal tissue. Along with their ability to modulate the local microenvironment, the introduction of properly differentiated mesenchymal stem cells into patients with Type 1 diabetes may provide a treatment option that helps supplement the lost islet cells without provoking an immune response. Preliminary clinical trials have shown that stem cell therapy decreases the amount of exogenous insulin required daily, decreases fasting glucose levels, decreases amount of glycated hemoglobin and increases C-peptide levels. These four indicators of diabetic control suggest that mesenchymal stem cells are an effective means of helping manage Type 1 diabetes. Still, much research needs to be done to fully understand the biomechanics behind the cells’ actions in order to expand human clinical trials. Although complete insulin independence is rarely achieved in patients receiving mesenchymal stem cell treatment, the promising results shown so far suggest more studies be undertaken in hopes of finding a corrective approach to treat Type 1 diabetes

You Think You Do, but You Don’t: An Investigation of Fandom and Nostalgic Media Through an Analysis of World of Warcraft Classic

The purpose of this study was to analyze the fan culture of the video game, World of Warcraft, to analyze the motivations, reactions, and reasons of play, to examine World of Warcraft: Classic as a form of nostalgic media, and to study its fandom through an ethnographic lens. This study utilized a fusion of the following methodologies: cyberethnography, ethnography, and visual ethnography. The author recorded gameplay footage of World of Warcraft, and World of Warcraft Classic. After this, an ethnography was conducted at the 2019 Blizzcon convention, in which observation shots and interviews took place. This footage was edited together to form a visual ethnography medium, in which the data and findings are presented. The results are that the popularity around World of Warcraft Classic is not because of the game itself, but rather the idea that it was a step in the right direction to giving the fans a sense of agency and say in the game’s future development

Probing Wolf-Rayet Winds: Chandra/HETG X-Ray Spectra of WR 6

With a deep Chandra/HETGS exposure of WR 6, we have resolved emission lines whose profiles show that the X-rays originate from a uniformly expanding spherical wind of high X-ray-continuum optical depth. The presence of strong helium-like forbidden lines places the source of X-ray emission at tens to hundreds of stellar radii from the photosphere. Variability was present in X-rays and simultaneous optical photometry, but neither were correlated with the known period of the system or with each other. An enhanced abundance of sodium revealed nuclear processed material, a quantity related to the evolutionary state of the star. The characterization of the extent and nature of the hot plasma in WR 6 will help to pave the way to a more fundamental theoretical understanding of the winds and evolution of massive stars.Comment: Accepted by the Astrophysical Journa

Close Pairs as Proxies for Galaxy Cluster Mergers

Galaxy cluster merger statistics are an important component in understanding the formation of large-scale structure. Unfortunately, it is difficult to study merger properties and evolution directly because the identification of cluster mergers in observations is problematic. We use large N-body simulations to study the statistical properties of massive halo mergers, specifically investigating the utility of close halo pairs as proxies for mergers. We examine the relationship between pairs and mergers for a wide range of merger timescales, halo masses, and redshifts (0<z<1). We also quantify the utility of pairs in measuring merger bias. While pairs at very small separations will reliably merge, these constitute a small fraction of the total merger population. Thus, pairs do not provide a reliable direct proxy to the total merger population. We do find an intriguing universality in the relation between close pairs and mergers, which in principle could allow for an estimate of the statistical merger rate from the pair fraction within a scaled separation, but including the effects of redshift space distortions strongly degrades this relation. We find similar behavior for galaxy-mass halos, making our results applicable to field galaxy mergers at high redshift. We investigate how the halo merger rate can be statistically described by the halo mass function via the merger kernel (coagulation), finding an interesting environmental dependence of merging: halos within the mass resolution of our simulations merge less efficiently in overdense environments. Specifically, halo pairs with separations less than a few Mpc/h are more likely to merge in underdense environments; at larger separations, pairs are more likely to merge in overdense environments.Comment: 12 pages, 9 figures; Accepted for publication in ApJ. Significant additions to text and two figures changed. Added new findings on the universality of pair mergers and added analysis of the effect of FoF linking length on halo merger

Size and Structure of the Narrow-Line Region of Quasars

We have observed the narrow-line regions (NLRs) of the seven brightest radio-quiet PG (or BQS) quasars (z < 0.5) with the Wide Field and Planetary Camera 2 on board the Hubble Space Telescope (HST). Linear-ramp filters were used to image the [OIII] lambda 5007 line emission with 0.0455-0.1 arcsec pixel resolution. We find that the NLRs are very compact with typical extents of 2-4 arcsec. Two quasars show compact filamentary structures similar to Seyfert NLRs. They may be related to radio outflows. Most interestingly, when including a sample of Seyfert galaxies observed with HST, we tentatively find that the size of the NLR is proportional to the square root of the [OIII] luminosity. This is comparable to the scaling found for the size of the broad-line region with continuum luminosity, which has been interpreted in terms of a constant photoionization parameter. The relation determined here connects the NLR of radio-quiet quasars and Seyferts over three orders of magnitude in [OIII] luminosity.Comment: 6 pages, 4 figures, accepted for publication in ApJ Letter

Src, PKCα, and PKCδ are required for αvβ3 integrin-mediated metastatic melanoma invasion

<p>Abstract</p> <p>Background</p> <p>Integrins, cell-surface receptors that mediate adhesive interactions between cells and the extracellular matrix (ECM), play an important role in cancer progression. Expression of the vitronectin receptor αvβ3 integrin correlates with increased invasive and metastatic capacity of malignant melanomas, yet it remains unclear how expression of this integrin triggers melanoma invasion and metastasis.</p> <p>Results</p> <p>Two melanoma cell lines C8161.9 and M14 both express high levels of αvβ3 integrin and adhere to vitronectin. However, only the highly metastatic C8161.9 cells are capable of invading vitronectin-enriched Matrigel in an αvβ3-depenent manner. Elevated levels of PKCα and PKCδ, and activated Src were detected specifically in the highly metastatic melanoma cells, but not in the low metastatic M14 cells. Inhibition of Src or PKC activity suppressed αvβ3-dependent invasion. Furthermore, over expression of Src or PKCα and PKCδ was sufficient to confer αvβ3-dependent invasiveness to M14 cells. Stress fiber formation and focal adhesion formation were almost completely absent in C8161.9 cells compared to M14 cells. Inhibition of Src signaling was sufficient to restore normal actin architecture, and resulted in decreased p190RhoGAP phosphorylation and enhanced RhoA activity. Src had no effect on Rac activity. Loss of PKCα expression, but not PKCδ, by siRNA inhibited Rac and PAK activity as well as invasiveness. Loss of PKCα restored focal adhesion formation and partially restored stress fiber formation, while loss of PKCδ primarily restored stress fibers.</p> <p>Conclusion</p> <p>The misregulated expression of PKCα and PKCδ and elevated Src activity in metastatic melanoma cells is required for efficient αvβ3-mediated invasion. PKCα and Src enhance αvβ3-mediated invasion in part by increasing the GTPase activity of Rac relative to RhoA. PKCα influences focal adhesion formation, while PKCδ controls stress fibers.</p

its goals, rationale, data infrastructure, and current developments

Background With multifaceted imaging capabilities, cardiovascular magnetic resonance (CMR) is playing a progressively increasing role in the management of various cardiac conditions. A global registry that harmonizes data from international centers, with participation policies that aim to be open and inclusive of all CMR programs, can support future evidence-based growth in CMR. Methods The Global CMR Registry (GCMR) was established in 2013 under the auspices of the Society for Cardiovascular Magnetic Resonance (SCMR). The GCMR team has developed a web-based data infrastructure, data use policy and participation agreement, data-harmonizing methods, and site-training tools based on results from an international survey of CMR programs. Results At present, 17 CMR programs have established a legal agreement to participate in GCMR, amongst them 10 have contributed CMR data, totaling 62,456 studies. There is currently a predominance of CMR centers with more than 10 years of experience (65%), and the majority are located in the United States (63%). The most common clinical indications for CMR have included assessment of cardiomyopathy (21%), myocardial viability (16%), stress CMR perfusion for chest pain syndromes (16%), and evaluation of etiology of arrhythmias or planning of electrophysiological studies (15%) with assessment of cardiomyopathy representing the most rapidly growing indication in the past decade. Most CMR studies involved the use of gadolinium-based contrast media (95%). Conclusions We present the goals, mission and vision, infrastructure, preliminary results, and challenges of the GCMR. Trial registration Identification number on ClinicalTrials.gov: NCT02806193. Registered 17 June 2016

Chromosome-wide identification of novel imprinted genes using microarrays and uniparental disomies

Genomic imprinting refers to a specialized form of epigenetic gene regulation whereby the expression of a given allele is dictated by parental origin. Defining the extent and distribution of imprinting across genomes will be crucial for understanding the roles played by imprinting in normal mammalian growth and development. Using mice carrying uniparental disomies or duplications, microarray screening and stringent bioinformatics, we have developed the first large-scale tissue-specific screen for imprinted gene detection. We quantify the stringency of our methodology and relate it to previous non-tissue-specific large-scale studies. We report the identification in mouse of four brain-specific novel paternally expressed transcripts and an additional three genes that show maternal expression in the placenta. The regions of conserved linkage in the human genome are associated with the Prader–Willi Syndrome (PWS) and Beckwith–Wiedemann Syndrome (BWS) where imprinting is known to be a contributing factor. We conclude that large-scale systematic analyses of this genre are necessary for the full impact of genomic imprinting on mammalian gene expression and phenotype to be elucidated

High resolution Chandra HETG and RXTE observations of GRS 1915+105 : A hot disk atmosphere & cold gas enriched in Iron and Silicon

The time-averaged 30 ks Chandra HETGS observation of the micro-quasar GRS 1915+105 in the low hard state reveals for the first time in this source neutral K absorption edges from Fe, Si, Mg, & S. Ionized resonance absorption from H-, and He-like Fe (XXV, XXVI), Ca XX and possibly emission from neutral Fe Kalpha and ionized Fe XXV (forbidden, or the resonance emission component of a P-Cygni profile) are also seen. We report the tentative detection of the first astrophysical signature of XAFS in the photoelectric edge of Si (and possibly Fe and Mg), attributed to material in grains. The large column densities measured from the neutral edges reveal anomalous Si and Fe abundances. Scenarios for which the anomalous abundances can be attributed to surrounding cold material associated with GRS 1915+105 and/or that the enrichment may signify either a highly unusual supernova/hypernova, or external supernova activity local to the binary are discussed. We attribute the ionized features to a hot disk, disk-wind, or corona environment. These features allow for constraints on the ionization parameter (log xi > 4.15), temperature (T > 2.4 x 10^6 K), and hydrogen equivalent number density (n > 10^{12} cm^{-3}) for this region. Variability studies with simultaneous RXTE data show that the light curve count rate tracks changes in the disk blackbody and the power-law flux. Spectral changes in the Chandra data also track the behavior of the light curve, and may point to changes in both the ionizing flux and density of the absorber. A 3.69 Hz QPO and weak first harmonic is seen in the RXTE data.Comment: Accepted for publication in ApJ., 9 pages, 5 figure