1,194 research outputs found
Diffusion and bulk flow in phloem loading - a theoretical analysis of the polymer trap mechanism in plants
Plants create sugar in the mesophyll cells of their leaves by photosynthesis.
This sugar, mostly sucrose, has to be loaded via the bundle sheath into the
phloem vascular system (the sieve elements), where it is distributed to growing
parts of the plant. We analyze the feasibility of a particular loading
mechanism, active symplasmic loading, also called the polymer trap mechanism,
where sucrose is transformed into heavier sugars, such as raffinose and
stachyose, in the intermediary-type companion cells bordering the sieve
elements in the minor veins of the phloem. Keeping the heavier sugars from
diffusing back requires that the plasmodesmata connecting the bundle sheath
with the intermediary cell act as extremely precise filters, which are able to
distinguish between molecules that differ by less than 20% in size. In our
modeling, we take into account the coupled water and sugar movement across the
relevant interfaces, without explicitly considering the chemical reactions
transforming the sucrose into the heavier sugars. Based on the available data
for plasmodesmata geometry, sugar concentrations and flux rates, we conclude
that this mechanism can in principle function. We find that the water flow
through the plasmodesmata, which has not been quantified before, contributes
only 10-20% to the sucrose flux into the intermediary cells, while the main
part is transported by diffusion. On the other hand, the subsequent sugar
translocation into the sieve elements would very likely be carried
predominantly by bulk water flow through the plasmodesmata. Thus, in contrast
to apoplasmic loaders, all the necessary water for phloem translocation would
be supplied in this way with no need for additional water uptake across the
plasma membranes of the phloem.Comment: 29 pages with 5 figure
Moving Bedforms Control CO2 Production and Distribution in Sandy River Sediments
Streams and rivers play an important role in the global carbon cycle. The origins of CO2 in streams are often poorly constrained or neglected, which is especially true for CO2 originating from heterotrophic metabolism in streambeds. We hypothesized that sediment movement will have a direct effect on stream metabolism, and thus, the aim of this study was to quantify the effect of moving bedforms on the production of CO2 in sandy streambeds. We conducted flume experiments where we used planar optodes to measure the distributions of O2 and CO2 under various streambed celerities. We combined these measurements with an assessment of bed morphodynamics and modeling to calculate O2 consumption and CO2 production rates. Our results indicate that sediment transport can strongly influence streambed metabolism and CO2 production. We found that bedform celerity controls the shape of the hyporheic zone and exchange flux, and is directly linked to the spatial and temporal distributions of O2 and CO2. It was also found that the most pronounced change in CO2 production occurred when the bed changed from stationary conditions to a slowly moving bed. A more gradual increase in O2 consumption and CO2 production rates was observed with further increase in celerity. Our study also points out that bedform movement causes hydraulic isolation between the moving and the nonâmoving fraction of the streambed that can lead to a transient storage of CO2 in deeper sediments, which may be released in bursts during bed scour.European UnionBMBFDFGIsrael Science Foundation
http://dx.doi.org/10.13039/501100003977Peer Reviewe
Evidence for temporal and spatial variations in groundwater contaminant plume discharge to a stream
Simultaneous attenuation of trace organics and change in organic matter composition in the hyporheic zone of urban streams
Trace organic compounds (TrOCs) enter rivers with discharge of treated wastewater. These effluents can contain high loads of dissolved organic matter (DOM). In a 48Â h field study, we investigated changes in molecular composition of seven DOM compound classes (FTICR-MS) and attenuation of 17 polar TrOCs in a small urban stream receiving treated wastewater. Correlations between TrOCs and DOM were used to identify simultaneous changes in surface water and the hyporheic zone. Changes in TrOC concentrations in surface water ranged between a decrease of 29.2% for methylbenzotriazole and an increase of 152.2% for the transformation product gabapentin-lactam. In the hyporheic zone, only decreasing TrOC concentrations were observed, ranging from 4.9% for primidone to 93.8% for venlafaxine . TrOC attenuation coincided with a decline of molecular diversity of easily biodegradable DOM compound classes while molecular diversity of poorly biodegradable DOM compound classes increased. This concurrence indicates similar or linked attenuation pathways for biodegradable DOM and TrOCs. Strong correlations between TrOCs and DOM compound classes as well as high attenuation of TrOCs primarily occurred in the hyporheic zone. This suggests high potential for DOM turnover and TrOC mitigation in rivers if hyporheic exchange is sufficient.German Research Foundation (DFG)European Union's Horizon 2020 research and innovation programme under Marie Sklodowska-Curie grant agreementBattelle Memorial Institute for the U.S. Department of EnergyPeer Reviewe
Seasonal Differences in the Attenuation of Polar Trace Organics in the Hyporheic Zone of an Urban Stream
Attenuation of trace organic compounds (TrOCs) in a river occurs to a large extent in its hyporheic zone. A major part of the attenuation of polar TrOCs is of microbial origin. As microbial activity depends on temperature and redox conditions, seasonal differences in TrOC attenuation are likely. We investigated TrOC attenuation at a river influenced by treated wastewater during two sampling campaigns, one in summer and one in winter. In addition to redox conditions and temperature, we also determined residence times of porewater in sediment using three methods: (a) nonâparametric deconvolution of electrical conductivity time series, (b) the model VFLUX 2.0 based on temperature time series (only summer), and (c) applying Darcy's law to differences in hydraulic heads (only summer). Contrary to our expectations, we found higher attenuation for 12 out of 18 TrOCs in winter, while three TrOCs were better attenuated in summer. Sediment conditions varied between seasons as more of the top sandy layer with a higher hydraulic permeability accumulated on the river bed in summer. As a result, residence times in the sediment were shorter in summer. In winter, longer residence times, lower temperatures, and a steeper oxygen gradient in sediment coincided with higher TrOC attenuation. Further research is needed to understand our unexpected findings and underlying mechanisms.Key Points
The attenuation of 12 out of 18 trace organic compounds (TrOCs) in the hyporheic zone was higher in winter while three TrOCs were attenuated better in summer
Residence times in sediment were longer and more diverse in winter
The extent of the oxic sediment was similar between seasons but the gradient from the oxic to anoxic zone was steeper in winterDeutsche Forschungsgemeinschaft (DFG)
http://dx.doi.org/10.13039/501100001659EC | H2020 | H2020 Priority Excellent Science | H2020 Marie SkĆodowskaâCurie Actions (MSCA)
http://dx.doi.org/10.13039/100010665University of Western Australia â University Postgraduate AwardAustralian Government Research Training Program ScholarshipBundesministerium fĂŒr Bildung und Forschung (BMBF)
http://dx.doi.org/10.13039/501100002347Peer Reviewe
Preconditions for Coping with Time and Performance Pressure in Different Ways
Time and performance pressure (TPP) is one of the most prevalent job demands in todaysâ working world. TPP often elicits coping behaviours of work extension (e.g., working overtime) and intensification (e.g., skipping breaks) that can be detrimental to employee health and well-being, at least in the long run. Therefore, a central research question is which preconditions employees need to cope differently, in a more problem-focused and healthy way with TPP. We addressed this question with research case studies in four different companies, integrating multiple stakeholders and using a multimethods approach. The primary method within the case studies were qualitative interviews with employees performing service or knowledge work. We could identify structural as well as climatic and personal factors. Factors lie on four different levels (organization, task, team, and individual) as important preconditons for alternative coping behaviours. Results of a two-step cross-case analysis show that preconditions on the task level, such as control at work, seem to be very central. Preconditions on the different levels are closely intertwined and some themes can be found across levels, such as âcontrol at and over workâ or the theme of âcareâ. The identified preconditions are important starting points for interventions to foster alternative coping with TPP
Nutritive und antinutritive Inhaltsstoffe in Körnerleguminosen: Einfluss von Jahr und Standort auf den Futterwert
Die meisten Körnerleguminosen enthalten antinutritive Substanzen, abhĂ€ngig von den Sorten und den Wachstumsbedingungen. Ziel der Studie war es, verschiedene Körnerleguminosenarten zu vergleichen, um ihren Futterwert unter BerĂŒcksichtigung ihrer nutritiven und antinutritiven Inhaltsstoffe zu ermitteln. Die Feldversuche wurden 2012 und 2013 in Karelshaff, Colmar-Berg, in Luxemburg und 2014 und 2015 an der Hessischen StaatsdomĂ€ne Frankenhausen in Grebenstein durchgefĂŒhrt. Erste Ergebnisse fĂŒr antinutritive Inhaltsstoffe zeigen, dass die Standorte und die jĂ€hrlichen Schwankungen einen Einfluss auf den Gehalt haben und stark von den in der Literatur angegebenen Werten abweichen können
Seasonal Differences in the Attenuation of Polar Trace Organics in the Hyporheic Zone of an Urban Stream
Attenuation of trace organic compounds (TrOCs) in a river occurs to a large extent in its hyporheic zone. A major part of the attenuation of polar TrOCs is of microbial origin. As microbial activity depends on temperature and redox conditions, seasonal differences in TrOC attenuation are likely. We investigated TrOC attenuation at a river influenced by treated wastewater during two sampling campaigns, one in summer and one in winter. In addition to redox conditions and temperature, we also determined residence times of porewater in sediment using three methods: (a) nonâparametric deconvolution of electrical conductivity time series, (b) the model VFLUX 2.0 based on temperature time series (only summer), and (c) applying Darcy's law to differences in hydraulic heads (only summer). Contrary to our expectations, we found higher attenuation for 12 out of 18 TrOCs in winter, while three TrOCs were better attenuated in summer. Sediment conditions varied between seasons as more of the top sandy layer with a higher hydraulic permeability accumulated on the river bed in summer. As a result, residence times in the sediment were shorter in summer. In winter, longer residence times, lower temperatures, and a steeper oxygen gradient in sediment coincided with higher TrOC attenuation. Further research is needed to understand our unexpected findings and underlying mechanisms.DFG, 248198858, GRK 2032: Grenzzonen in urbanen WassersystemenEC/H2020/765553/EU/A EUROpean training and research network for environmental FLOW management in river basins/EUROFLO
Review on Chamber-Specific Differences in Right and Left Heart Reactive Oxygen Species Handling
Reactive oxygen species (ROS) exert signaling character (redox signaling), or damaging character (oxidative stress) on cardiac tissue depending on their concentration and/or reactivity. The steady state of ROS concentration is determined by the interplay between its production (mitochondrial, cytosolic, and sarcolemmal enzymes) and ROS defense enzymes (mitochondria, cytosol). Recent studies suggest that ROS regulation is different in the left and right ventricle of the heart, specifically by a different activity of superoxide dismutase (SOD). Mitochondrial ROS defense seems to be lower in right ventricular tissue compared to left ventricular tissue. In this review we summarize the current evidence for heart chamber specific differences in ROS regulation that may play a major role in an observed inability of the right ventricle to compensate for cardiac stress such as pulmonary hypertension. Based on the current knowledge regimes to increase ROS defense in right ventricular tissue should be in the focus for the development of future therapies concerning right heart failure
Task sharing in an interprofessional medication management program â a survey of general practitioners and community pharmacists
Background
Pharmacist-led medication review and medication management programs (MMP) are well-known strategies to improve medication safety and effectiveness. If performed interprofessionally, outcomes might even improve. However, little is known about task sharing in interprofessional MMP, in which general practitioners (GPs) and community pharmacists (CPs) collaboratively perform medication reviews and continuously follow-up on patients with designated medical and pharmaceutical tasks, respectively. In 2016, ARMIN (Arzneimittelinitiative Sachsen-ThĂŒringen) an interprofessional MMP was launched in two German federal states, Saxony and Thuringia. The aim of this study was to understand how GPs and CPs share tasks in MMP when reviewing the patientsâ medication.
Methods
This was a cross-sectional postal survey among GPs and CPs who participated in the MMP. Participants were asked who completed which MMP tasks, e.g., checking drug-drug interactions, dosing, and side effects. In total, 15 MMP tasks were surveyed using a 5-point Likert scale ranging from âI complete this task aloneâ to âGP/CP completes this task aloneâ. The study was conducted between 11/2020 and 04/2021. Data was analyzed using descriptive statistics.
Results
In total, 114/165 (69.1%) GPs and 166/243 (68.3%) CPs returned a questionnaire. The majority of GPs and CPs reported (i) checking clinical parameters and medication overuse and underuse to be completed by GPs, (ii) checking storage conditions of drugs and initial compilation of the patientâs medication including brown bag review being mostly performed by CPs, and (iii) checking side-effects, non-adherence, and continuous updating of the medication list were carried out jointly. The responses differed most for problems with self-medication and adding and removing over-the-counter medicines from the medication list. In addition, the responses revealed that some MMP tasks were not sufficiently performed by either GPs or CPs.
Conclusions
Both GPsâ and CPsâ expertise are needed to perform MMP as comprehensively as possible. Future studies should explore how GPs and CPs can complement each other in MMP most efficiently
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