Relation of endocrine gland weight to body weight in growing and mature female dairy goats

Digitized 2007 AES.Includes bibliographical references (page 22)

The Use of Cafeteria Trials for the Selection of \u3ci\u3eDesmodium ovalifolium\u3c/i\u3e Genotypes

For the selection of tropical legumes which contain anti-nutritive components such as tannins, relative acceptability of genotypes to ruminants is of particular importance, since these plant components may influence selective grazing behaviour and subsequent animal productivity. Plant-animal interactions are not predictable from laboratory analyses. Involving grazing animals through the conduction of relative-acceptability (=cafeteria) trials at early stages of the germplasm selection process might therefore provide a convenient tool to adjust and confirm genotype selection based on laboratory quality analyses data. As part of a multilocational germplasm evaluation project, cafeteria-experiments were conducted at two contrasting environments in Colombia with a core collection of Desmodium ovalifolium, a tropical legume species containing tannins. The objective of these experiments was to assess the usefulness of such acceptability trials in the selection of D. ovalifolium genotypes. Relative acceptability indices for the 18 accessions confirm genotype selection based on a series of laboratory quality analyses during earlier stages of the project and indicate pronounced genotype-environment interactions. Moreover, animal activity profiles confirm the influence of plant-environment-animal interactions and thus the usefulness of cafeteriatrials for germplasm selection projects

Fetal calf serum heat inactivation and lipopolysaccharide contamination influence the human T lymphoblast proteome and phosphoproteome

<p>Abstract</p> <p>Background</p> <p>The effects of fetal calf serum (FCS) heat inactivation and bacterial lipopolysaccharide (LPS) contamination on cell physiology have been studied, but their effect on the proteome of cultured cells has yet to be described. This study was undertaken to investigate the effects of heat inactivation of FCS and LPS contamination on the human T lymphoblast proteome. Human T lymphoblastic leukaemia (CCRF-CEM) cells were grown in FCS, either non-heated, or heat inactivated, having low (< 1 EU/mL) or regular (< 30 EU/mL) LPS concentrations. Protein lysates were resolved by 2-DE followed by phospho-specific and silver nitrate staining. Differentially regulated spots were identified by nano LC ESI Q-TOF MS/MS analysis.</p> <p>Results</p> <p>A total of four proteins (EIF3M, PRS7, PSB4, and SNAPA) were up-regulated when CCRF-CEM cells were grown in media supplemented with heat inactivated FCS (HE) as compared to cells grown in media with non-heated FCS (NHE). Six proteins (TCPD, ACTA, NACA, TCTP, ACTB, and ICLN) displayed a differential phosphorylation pattern between the NHE and HE groups. Compared to the low concentration LPS group, regular levels of LPS resulted in the up-regulation of three proteins (SYBF, QCR1, and SUCB1).</p> <p>Conclusion</p> <p>The present study provides new information regarding the effect of FCS heat inactivation and change in FCS-LPS concentration on cellular protein expression, and post-translational modification in human T lymphoblasts. Both heat inactivation and LPS contamination of FCS were shown to modulate the expression and phosphorylation of proteins involved in basic cellular functions, such as protein synthesis, cytoskeleton stability, oxidative stress regulation and apoptosis. Hence, the study emphasizes the need to consider both heat inactivation and LPS contamination of FCS as factors that can influence the T lymphoblast proteome.</p

The Influence of Length of Storage of Bovine Semen on Conception Rate under Field Conditions

It is the purpose of this study to determine whether under conditions of routine handling in artificial breeding associations there is a decline in semen fertility from day to day and also to determine the magnitude of this decline. Tabulation of such data will not only be of value in determining the effect of frequent semen collection on the over-all artificial breeding results but will also serve as a basis of comparison in investigational work directed toward increasing the efficiency of artificial breeding

Influence of mental health literacy on help-seeking behaviour for mental health problems in the Swiss young adult community: a cohort and longitudinal case–control study

Poor knowledge about mental health disorders and their treatment likely contributes to the large treatment gap reported for mental health problems. Therefore, we studied the association between mental health literacy (MHL) and active help-seeking in a community sample. Participants were recruited from an add-on questionnaire study to the 'Bern Epidemiological At-Risk' (BEAR) study on 16-40-year-old community subjects of the Swiss canton Bern. At baseline, data of N = 1504, and at 3-year follow-up, data of N = 535 were available. Based on an unlabelled case vignette (on depression or schizophrenia), MHL was assessed by the questionnaire of Angermeyer and colleagues. Cross-sectional and longitudinal baseline predictors of help-seeking were analysed using path analyses. Additionally, sensitivity analyses of the prospective model were computed for sex, vignette, and baseline mental health problems/disorders. Cross-sectionally, help-seeking was associated with non-endorsement of biogenetic causal explanations, presence of mental health problems/disorders, help-seeking before baseline, poorer functioning, and lower health satisfaction. The prospective model was similar; yet, help-seeking at follow-up was associated with endorsements of the causal explanation 'biogenetics' and, additionally, 'childhood trauma' but not the presence of baseline mental health problems/disorders. Sensitivity analyses revealed a significant impact on sex, vignette, and mental health problems/disorders. For example, actual functional problems were predictive in males, while health satisfaction was predictive in females. Our findings indicate that future studies on drivers of help-seeking should assess very large community samples with case vignettes on different mental disorders to examine appropriate subgroups and their likely interaction to address group-specific factors in awareness campaigns