108 research outputs found
Complete genome sequence and metabolic potential of the quinaldine-degrading bacterium Arthrobacter sp. Rue61a
Background: Bacteria of the genus Arthrobacter are ubiquitous in soil environments and can be considered as true survivalists. Arthrobacter sp. strain Rue61a is an isolate from sewage sludge able to utilize quinaldine (2- methylquinoline) as sole carbon and energy source. The genome provides insight into the molecular basis of the versatility and robustness of this environmental Arthrobacter strain.
Results: The genome of Arthrobacter sp. Rue61a consists of a single circular chromosome of 4,736,495 bp with an average G + C content of 62.32%, the circular 231,551-bp plasmid pARUE232, and the linear 112,992-bp plasmid pARUE113 that was already published. Plasmid pARUE232 is proposed to contribute to the resistance of Arthrobacter sp. Rue61a to arsenate and Pb2+, whereas the linear plasmid confers the ability to convert quinaldine to anthranilate. Remarkably, degradation of anthranilate exclusively proceeds via a CoA-thioester pathway. Apart from quinaldine utilization, strain Rue61a has a limited set of aromatic degradation pathways, enabling the utilization of 4-hydroxy-substituted aromatic carboxylic acids, which are characteristic products of lignin depolymerization, via ortho cleavage of protocatechuate. However, 4-hydroxyphenylacetate degradation likely proceeds via meta cleavage of homoprotocatechuate. The genome of strain Rue61a contains numerous genes associated with osmoprotection, and a high number of genes coding for transporters. It encodes a broad spectrum of enzymes for the uptake and utilization of various sugars and organic nitrogen compounds. A. aurescens TC-1 is the closest sequenced relative of strain Rue61a. Conclusions: The genome of Arthrobacter sp. Rue61a reflects the saprophytic lifestyle and nutritional versatility of the organism and a strong adaptive potential to environmental stress. The circular plasmid pARUE232 and the linear plasmid pARUE113 contribute to heavy metal resistance and to the ability to degrade quinaldine, respectively
An upper limit on hypertriton production in collisions of Ar(1.76 AGeV)+KCl
A high-statistic data sample of Ar(1.76 AGeV)+KCl events recorded with HADES
is used to search for a hypertriton signal. An upper production limit per
centrality-triggered event of x on the level is
derived. Comparing this value with the number of successfully reconstructed
hyperons allows to determine an upper limit on the ratio
, which is confronted with statistical and
coalescence-type model calculations
Searching a Dark Photon with HADES
We present a search for the e+e- decay of a hypothetical dark photon, also
names U vector boson, in inclusive dielectron spectra measured by HADES in the
p (3.5 GeV) + p, Nb reactions, as well as the Ar (1.756 GeV/u) + KCl reaction.
An upper limit on the kinetic mixing parameter squared epsilon^{2} at 90% CL
has been obtained for the mass range M(U) = 0.02 - 0.55 GeV/c2 and is compared
with the present world data set. For masses 0.03 - 0.1 GeV/c^2, the limit has
been lowered with respect to previous results, allowing now to exclude a large
part of the parameter region favoured by the muon g-2 anomaly. Furthermore, an
improved upper limit on the branching ratio of 2.3 * 10^{-6} has been set on
the helicity-suppressed direct decay of the eta meson, eta-> e+e-, at 90% CL
The interaction studied via femtoscopy in p + Nb reactions at
We report on the first measurement of and correlations via
the femtoscopy method in p+Nb reactions at , studied with the High Acceptance Di-Electron Spectrometer
(HADES). By comparing the experimental correlation function to model
calculations, a source size for pairs of and a slightly
smaller value for of is extracted.
Using the geometrical extent of the particle emitting region, determined
experimentally with correlations as reference together with a source
function from a transport model, it is possible to study different sets of
scattering parameters. The correlation is proven sensitive to
predicted scattering length values from chiral effective field theory. We
demonstrate that the femtoscopy technique can be used as valid alternative to
the analysis of scattering data to study the hyperon-nucleon interaction.Comment: 12 pages, 11 figure
Genome sequence analyses of two isolates from the recent Escherichia coli outbreak in Germany reveal the emergence of a new pathotype: Entero-Aggregative-Haemorrhagic Escherichia coli (EAHEC)
The genome sequences of two Escherichia coli O104:H4 strains derived from two different patients of the 2011 German E. coli outbreak were determined. The two analyzed strains were designated E. coli GOS1 and GOS2 (German outbreak strain). Both isolates comprise one chromosome of approximately 5.31 Mbp and two putative plasmids. Comparisons of the 5,217 (GOS1) and 5,224 (GOS2) predicted protein-encoding genes with various E. coli strains, and a multilocus sequence typing analysis revealed that the isolates were most similar to the entero-aggregative E. coli (EAEC) strain 55989. In addition, one of the putative plasmids of the outbreak strain is similar to pAA-type plasmids of EAEC strains, which contain aggregative adhesion fimbrial operons. The second putative plasmid harbors genes for extended-spectrum β-lactamases. This type of plasmid is widely distributed in pathogenic E. coli strains. A significant difference of the E. coli GOS1 and GOS2 genomes to those of EAEC strains is the presence of a prophage encoding the Shiga toxin, which is characteristic for enterohemorrhagic E. coli (EHEC) strains. The unique combination of genomic features of the German outbreak strain, containing characteristics from pathotypes EAEC and EHEC, suggested that it represents a new pathotype Entero-Aggregative-Haemorrhagic Escherichiacoli (EAHEC)
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