62 research outputs found

    Replik zur Rezension von Hans-Ulrich Musolff zum Band Rüdiger Schnell (Hrsg.): Zivilsationsprozesse. Zu Erziehungsschriften in der Vormoderne. Köln, Weimar, Wien: Böhlau 2005 (347 S.) [Rezension]

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    Replik zur Rezension von Hans-Ulrich Musolff zum Band Rüdiger Schnell (Hrsg.): Zivilsationsprozesse. Zu Erziehungsschriften in der Vormoderne. Köln, Weimar, Wien: Böhlau 2005 (347 S.; ISBN 3-412-13904-1; 34,90 EUR)

    Exploring Chromophore-Binding Pocket: High-Resolution Solid-State 1H–13C Interfacial Correlation NMR Spectra with Windowed PMLG Scheme

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    High-resolution two-dimensional (2D) 1H–13C heteronuclear correlation spectra are recorded for selective observation of interfacial 3–5.5 Å contacts of the uniformly 13C-labeled phycocyanobilin (PCB) chromophore with its unlabeled binding pocket. The experiment is based on a medium- and long-distance heteronuclear correlation (MELODI–HETCOR) method. For improving 1H spectral resolution, a windowed phase-modulated Lee–Goldburg (wPMLG) decoupling scheme is applied during the t1 evolution period. Our approach allows for identification of chromophore–protein interactions, in particular for elucidation of the hydrogen-bonding networks and charge distributions within the chromophore-binding pocket. The resulting pulse sequence is tested on the cyanobacterial (Cph1) phytochrome sensory module (residues 1–514, Cph1Δ2) containing uniformly 13C- and 15N-labeled PCB chromophore (u-[13C,15N]-PCB-Cph1Δ2) at 17.6 T

    Role of Myosin Va in the Plasticity of the Vertebrate Neuromuscular Junction In Vivo

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    Background: Myosin Va is a motor protein involved in vesicular transport and its absence leads to movement disorders in humans (Griscelli and Elejalde syndromes) and rodents (e.g. dilute lethal phenotype in mice). We examined the role of myosin Va in the postsynaptic plasticity of the vertebrate neuromuscular junction (NMJ). Methodology/Principal Findings: Dilute lethal mice showed a good correlation between the propensity for seizures, and fragmentation and size reduction of NMJs. In an aneural C2C12 myoblast cell culture, expression of a dominant-negative fragment of myosin Va led to the accumulation of punctate structures containing the NMJ marker protein, rapsyn-GFP, in perinuclear clusters. In mouse hindlimb muscle, endogenous myosin Va co-precipitated with surface-exposed or internalised acetylcholine receptors and was markedly enriched in close proximity to the NMJ upon immunofluorescence. In vivo microscopy of exogenous full length myosin Va as well as a cargo-binding fragment of myosin Va showed localisation to the NMJ in wildtype mouse muscles. Furthermore, local interference with myosin Va function in live wildtype mouse muscles led to fragmentation and size reduction of NMJs, exclusion of rapsyn-GFP from NMJs, reduced persistence of acetylcholine receptors in NMJs and an increased amount of punctate structures bearing internalised NMJ proteins. Conclusions/Significance: In summary, our data show a crucial role of myosin Va for the plasticity of live vertebrate neuromuscular junctions and suggest its involvement in the recycling of internalised acetylcholine receptors back to th

    L'amour courtois en tant que discours courtois sur l'amour (I)

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    Schnell Rüdiger. L'amour courtois en tant que discours courtois sur l'amour (I). In: Romania, tome 110 n°437-438, 1989. pp. 72-126

    L'amour courtois en tant que discours courtois sur l'amour (II)

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    Schnell Rüdiger. L'amour courtois en tant que discours courtois sur l'amour (II). In: Romania, tome 110 n°439-440, 1989. pp. 331-363
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