The patatin-like lipase family in Gallus gallus

<p>Abstract</p> <p>Background</p> <p>In oviparous species, genes encoding proteins with functions in lipid remodeling, such as specialized lipases, may have evolved to facilitate the assembly and utilization of yolk lipids by the embryo. The mammalian gene family of patatin-like phospholipases (PNPLAs) has received significant attention, but studies in other vertebrates are lacking; thus, we have begun investigations of PNPLA genes in the chicken (<it>Gallus gallus</it>).</p> <p>Results</p> <p>We scanned the draft chicken genome using human PNPLA sequences, and performed PCR to amplify and sequence orthologous cDNAs. Full-length cDNA sequences of galline <it>PNPLA2/ATGL, PNPLA4, -7, -8, -9</it>, and the activator protein <it>CGI-58</it>, as well as partial cDNA sequences of avian <it>PNPLA1, -3</it>, and <it>-6 </it>were obtained. The high degree of sequence identities (~50 to 80%) between the avian and human orthologs suggests conservation of important enzymatic functions. Quantitation by qPCR of the transcript levels of <it>PNPLA</it>s and <it>CGI-58 </it>in 21 tissues indicates that expression patterns and levels diverge greatly between species. A particularly interesting tissue in which certain PNPLAs may contribute to physiological specialization is the extraembryonic yolk sac.</p> <p>Conclusion</p> <p>Knowledge about the exact <it>in-vivo </it>functions of PNPLAs in any system is still sparse. Thus, studies about the temporal expression patterns and functions of the enzymes identified here, and of other already known extracellular lipases and co-factors, in the yolk sac and embryonic tissues during embryogenesis are called for. Based on the information obtained, further studies are anticipated to provide important insights of the roles of PNPLAs in the yolk sac and embryo development.</p

Evaluation of KMU.DIGITAL Module Consulting (WKÖ) and Module Implementation (AWS). Synthesis Report

Die vorliegende Analyse betrifft die Evaluierung des Förderungsprogramms KMU.DIGITAL mit den beiden Modulen Beratung und Umsetzung seit dem Programm-Relaunch KMU.DIGITAL 2.0 (September 2019 bis Dezember 2022). Das Evaluierungsziel liegt in der Beurteilung des Programms hinsichtlich Wirkung und Reichweite sowie evidenzbasierten Empfehlungen für die Fortführung und künftige Ausgestaltung des Programms. Der Evaluierungszeitraum erstreckte sich von Jänner bis Juli 2023

Evaluierung des Förderungsprogramms KMU.DIGITAL Modul Beratung (WKÖ) und Modul Umsetzung (AWS). Synthesebericht.

Die vorliegende Analyse betrifft die Evaluierung des Förderungsprogramms KMU.DIGITAL mit den beiden Modulen Beratung und Umsetzung seit dem Programm-Relaunch KMU.DIGITAL 2.0 (September 2019 bis Dezember 2022). Das Evaluierungsziel liegt in der Beurteilung des Programms hinsichtlich Wirkung und Reichweite sowie evidenzbasierten Empfehlungen für die Fortführung und künftige Ausgestaltung des Programms. Der Evaluierungszeitraum erstreckte sich von Jänner bis Juli 2023

European Survey on Scholarly Practices and Digital Needs in the Arts and Humanities

This report summarizes the statistical analysis of the findings of a web-based survey conducted by the Digital Methods and Practices Observatory (DiMPO), a working group under VCC2 of the DARIAH research infrastructure (Digital Research Infrastructure for the Arts and Humanities). In order to provide an evidence-based, up-to-date, and meaningful account of the emerging information practices, needs and attitudes of arts and humanities researchers in the evolving European digital scholarly environment, the web survey involved a transnational team of researchers from more than a dozen countries, and addressed digitally-enabled research practices, attitudes and needs in all areas of Europe and across different arts and humanities disciplines and contexts

Madgwas: a Database of Ethiopian Binding Decoration

Abstract and poster of paper 0555 presented at the Digital Humanities Conference 2019 (DH2019), Utrecht , the Netherlands 9-12 July, 2019

Madgwas: a Database of Ethiopian Binding Decoration

Abstract and poster of paper 0555 presented at the Digital Humanities Conference 2019 (DH2019), Utrecht , the Netherlands 9-12 July, 2019

Ontology for Adjective-Adverb Interfaces in Romance

An ontology that represents the shared annotation model developed within the research group on Adjective-Adverb Interfaces in Romance. The annotation model offers a cross‑linguistic categorization based on the forms, functions and meanings of the heterogeneous and multifunctional word‑class adverb, which could also be applied to other languages besides Romance