Evaluation of Impact from Ripple and Transient Phenomena in the LHC Dipole Strings

Based on measurements of the A.C. electrical characteristics of prototype magnets, synthesized computer models of the principal, individual, superconducting magnetic elements of the Large Hadron Collider have been elaborated. The models constitute the basic elements for the determination of the transfer functions of the magnet chains. This tool provides the possibility to evaluate the resonance spectrum of the chains and to determine the needs for additional damping. Results of the analysis of transmission line effects in the LHC main dipole chains are presented. In particular, data from ripple calculations and ramping studies are discussed. Computer simulations of the quench protection circuit provide information about the transient phenomena occurring in the dipole chains during normal operation of the fast de-excitation switches and under abnormal conditions

How Sensitive are Di-Leptons from Rho Mesons to the High Baryon Density Region?

We show that the measurement of di-leptons might provide only a restricted view into the most dense stages of heavy ion reactions. Thus, possible studies of meson and baryon properties at high baryon densities, as e.g. done at GSI-HADES and envisioned for FAIR-CBM, might observe weaker effects than currently expected in certain approaches. We argue that the strong absorption of resonances in the high baryon density region of the heavy ion collision masks information from the early hot and dense phase due to a strong increase of the total decay width because of collisional broadening. To obtain additional information, we also compare the currently used approaches to extract di-leptons from transport simulations - i.e. shining, only vector mesons from final baryon resonance decays and instant emission of di-leptons and find a strong sensitivity on the method employed in particular at FAIR and SPS energies. It is shown explicitly that a restriction to rho meson (and therefore di-lepton) production only in final state baryon resonance decays provide a strong bias towards rather low baryon densities. The results presented are obtained from UrQMD v2.3 calculations using the standard set-up.Comment: 8 pages, 6 figures, expanded versio

Energy Extraction for the LHC Superconducting Circuits

The superconducting magnets of the LHC will be powered in about 1700 electrical circuits. The energy stored in circuits, up to 1.3 GJ, can potentially cause severe damage of magnets, bus bars and current leads. In order to protect the superconducting elements after a resistive transition, the energy is dissipated into a dump resistor installed in series with the magnet chain that is switched into the circuit by opening current breakers. Experiments and simulation studies have been performed to identify the LHC circuits that need energy extraction. The required values of the extraction resistors have been computed. The outcome of the experimental results and the simulation studies are presented and the design of the different energy extraction systems that operate at 600 A and at 13 kA is described

Deficits in growth, muscle mass, and body composition following placental insufficiency-induced intrauterine growth restriction persisted in lambs at 60 d of age but were improved by daily clenbuterol supplementation

Low birthweight in livestock results from stress-induced intrauterine growth restriction (IUGR; Yates et al., 2018). IUGR fetuses exhibit diminished muscle growth that persists in the neonatal stage, leading to asymmetric body composition and decreased weight gain (Cadaret et al., 2019). Ultimately, low birthweight diminishes yield and carcass merit at harvest (Greenwood et al., 2000), making effective postnatal treatment strategies to improve IUGR growth outcomes necessary. In this study, we examined the benefits of injecting the β2 agonist clenbuterol daily to target adrenergic adaptations that we previously observed in IUGR muscle (Posont et al., 2018; Yates et al., 2018). We hypothesized that IUGRinduced growth deficits would persist at the juvenile stage, manifesting in inferior body composition and carcass traits. We also postulated that clenbuterol would at least partially recover growth and body symmetry. Our objective was to test this hypothesis by assessing growth metrics and body composition in IUGR-born lambs hand-reared to 60 d of age and supplemented daily with injectable clenbuterol

The Protection System for the Superconducting Elements of the Large Hadron Collider at CERN

The protection system for the superconducting elements of the Large Hadron Collider (LHC) [1] at the European Laboratory for Particle Physics (CERN), and its associated equipment are presented: quench detectors, cold diodes, quench heaters and related power supplies, extraction resistors and associated current breakers. Features such as radiation resistance, redundancy and required reliability are discussed

Beta-adrenergic agonists increase maximal output of oxidative phosphorylation in bovine satellite cells

Beta-adrenergic agonists (βAA), Ractopamine HCl (RH) and Zilpaterol HCl (ZH), are FDAapproved supplements utilized in pigs and cattle to improve growth performance, carcass weight, and longissimus muscle area (Arp et al., 2014; Lean et al., 2014). Previous studies within our group have focused on understanding molecular changes in skeletal muscle due to βAA supplementation. This work has shown that βAA supplementation increases glucose oxidation in muscle from thermoneutral and heat-stressed lambs (Barnes et al., 2019) and in rat skeletal muscle stimulated with ZH (Cadaret et al., 2017). Skeletal muscle transcriptomics of lambs supplemented ZH revealed upregulation of genes associated with the callipyge phenotype of sheep (Yu et al., 2018) as well as the upregulation of mitochondrial solute carrier SLC25A25 (Kubik et al., 2018). SLC25A25 is a Ca2+ sensitive ATP-Mg2+/Pi inner mitochondrial membrane solute transporter. Due to the role of the mitochondria in metabolism and the results of prior transcriptomics studies, the objective of this study was to understand how βAA affect mitochondrial function of bovine skeletal muscle stem (i.e., satellite) cells. We hypothesized that βAA would improve efficiency and ATP production capacity of muscle stem cells by modifying mitochondrial function

Beta-adrenergic agonists alter oxidative phosphorylation in primary myoblasts (Short Communication)

Beta-adrenergic agonists (β-AAs) are widely used supplements in beef and pork production to improve feed efficiency and increase lean muscle mass, yet little is known about the molecular mechanism by which β-AAs achieve this outcome. Our objective was to identify the influence of ractopamine HCl and zilpaterol HCl on mitochondrial respiratory activity in muscle satellite cells isolated from crossbred beef steers (N = 5), crossbred barrows (N = 2), Yorkshire-cross gilts (N = 3), and commercial weather lambs (N = 5). Real-time measurements of oxygen con­sumption rates (OCRs) were recorded using extracellular flux analyses with a Seahorse XFe24 analyzer. After basal OCR measurements were recorded, zilpaterol HCl, ractopamine HCl, or no β-AA was injected into the assay plate in three technical replicates for each cell isolate. Then, oligomycin, carbonyl cyanide-p-trifluoromethoxyphenylhydrazone, and rotenone were injected into the assay plate sequentially, each inducing a different cellular state. This allowed for the measurement of OCR at these states and for the calculation of the following measures of mitochon­drial function: basal respiration, non-mitochondrial respiration, maximal respiration, proton leak, adenosine triphosphate (ATP)-linked respiration, and spare respiratory capacity. Incubation of bovine cells with either zilpaterol HCl or ractopamine HCl increased maximal respiration (P = 0.046) and spare respiratory capacity (P = 0.035) compared with non-supplemented counterparts. No difference (P \u3e 0.05) was observed between zilpaterol HCl and ractopamine HCl for maximal respiration and spare respiratory capacity in bovine cell isolates. No measures of mitochondrial function (basal respiration, non-mitochondrial respiration, maximal respiration, proton leak, ATP-linked respiration, and spare respiratory capacity) were altered by β-AA treatment in ovine or porcine cells. These findings indicate that β-AAs in cattle may improve the efficiency of oxidative metabolism in muscle satellite cells by modifying mitochondrial respiratory activity. The lack of response by ovine and porcine cells to β-AA incubation also demonstrates differing physiological responses to β-AA across species, which helps to explain the variation in its effectiveness as a growth supplement. Lay Summary — Beta-adrenergic agonists (β-AAs) are supplemented to pigs and cattle to improve growth performance, carcass weight, and loin muscle area. Little is known about the mechanism taking place within individual cells by which β-AAs achieve this outcome. Previous work reported that β-AA supplementation improves the efficiency in which cells use glucose as an energy source and alters the expression of genes related to mitochondrial function, a key component of cellular energy production. To further our understanding of the impact of β-AA supplementation on these cellular functions, our objective was to identify the influence of two β-AAs used in livestock production, ractopamine HCl and zilpaterol HCl, on the mitochondrial respiratory activity of cells collected from the loin muscle and grown in culture. We isolated cells from cattle, pig, and sheep muscle and measured the oxygen consumption of the cells after treatment with ractopamine HCl, zilpaterol HCl, or with no supplement. We found that both ractopamine HCl and zilpaterol HCl enhance the efficiency of cellular energy production during a state of cellular stress in bovine muscle cells. There was no appreciable effect of the supplement on the energy production of pig or sheep cells. These data indicate that β-AA supplementation in cattle may increase the muscle cell energy production capacity compared with non-supplemented cells. This study also demonstrates that the efficiency of cell energy production is one plausible mechanism underlying species differences in the response to β-AA supplementation