Longing : Fox-Trot Song

https://digitalcommons.library.umaine.edu/mmb-vp/2023/thumbnail.jp

Dreamy Carolina Moon

Illustration of houses with field and large moon in backgroundhttps://scholarsjunction.msstate.edu/cht-sheet-music/12463/thumbnail.jp

Mother\u27s Love

Illustration of woman looking at moon by pondhttps://scholarsjunction.msstate.edu/cht-sheet-music/6278/thumbnail.jp

The Serine/threonine kinase Stk33 exhibits autophosphorylation and phosphorylates the intermediate filament protein Vimentin

<p>Abstract</p> <p>Background</p> <p>Colocalization of Stk33 with vimentin by double immunofluorescence in certain cells indicated that vimentin might be a target for phosphorylation by the novel kinase Stk33. We therefore tested <it>in vitro </it>the ability of Stk33 to phosphorylate recombinant full length vimentin and amino-terminal truncated versions thereof. In order to prove that Stk33 and vimentin are also <it>in vivo </it>associated proteins co-immunoprecipitation experiments were carried out. For testing the enzymatic activity of immunoprecipitated Stk33 we incubated precipitated Stk33 with recombinant vimentin proteins. To investigate whether Stk33 binds directly to vimentin, an <it>in vitro </it>co-sedimentation assay was performed.</p> <p>Results</p> <p>The results of the kinase assays demonstrate that Stk33 is able to specifically phosphorylate the non-α-helical amino-terminal domain of vimentin <it>in vitro</it>. Furthermore, co-immunoprecipitation experiments employing cultured cell extracts indicate that Stk33 and vimentin are associated <it>in vivo</it>. Immunoprecipitated Stk33 has enzymatic activity as shown by successful phosphorylation of recombinant vimentin proteins. The results of the co-sedimentation assay suggest that vimentin binds directly to Stk33 and that no additional protein mediates the association.</p> <p>Conclusion</p> <p>We hypothesize that Stk33 is involved in the <it>in vivo </it>dynamics of the intermediate filament cytoskeleton by phosphorylating vimentin.</p

Carolina Sunshine

[Verse 1]Down in Carolina where the sunbeams play Down where all the world seems bright Pickaninies romping all the day In the cotton fields of white How my heart is yearning to be there once more Just to hear the darkies song Carolina sunshine calls me back to the place where I belong [Chorus] Carolina Sun I’m lonesome Carolina Sun I’m blue When the day is done I’m pining For a sight of you Makes no diff’rence where I wander Any place I chance to roam When the golden sun sinks in the West Then I think of home [Chorus

The Meaning of Your A-B-C

Illustration of children looking to cross the street. Illustration of a man holding a child with people looking on.https://scholarsjunction.msstate.edu/cht-sheet-music/10470/thumbnail.jp

Dreamy Paradise

Illustration of man playing ukulele for woman in grass dresshttps://scholarsjunction.msstate.edu/cht-sheet-music/12453/thumbnail.jp

Mississippi Mammy

Illustration of woman sitting at cabin with flowers found cabinhttps://scholarsjunction.msstate.edu/cht-sheet-music/9259/thumbnail.jp

Drifting and Dreaming

Illustration of man standing in water with palm trees and moon in backgroundhttps://scholarsjunction.msstate.edu/cht-sheet-music/12466/thumbnail.jp