Historische Nachricht von dem am 16. u[nd] 17. Jul[i] 1724 zu Thorn in Preussen passirten Tumult des gemeinen Volcks, wider das Jesuiter-Collegium, Und der hierauff am 7. Dec[ember] Erfolgten scharffen Execution einiger zum Todt verurtheilten Personen, aus sichern geschriebenen und gedruckten Nachrichten, [...] Cz. 1

[1] k., 694 s.

Comparative Lipid Profiling of the Cnidarian <em>Aiptasia pallida</em> and Its Dinoflagellate Symbiont

<div><p>Corals and other cnidarians house photosynthetic dinoflagellate symbionts within membrane-bound compartments inside gastrodermal cells. Nutritional interchanges between the partners produce carbohydrates and lipids for metabolism, growth, energy stores, and cellular structures. Although lipids play a central role in the both the energetics and the structural/morphological features of the symbiosis, previous research has primarily focused on the fatty acid and neutral lipid composition of the host and symbiont. In this study we conducted a mass spectrometry-based survey of the lipidomic changes associated with symbiosis in the sea anemone <i>Aiptasia pallida</i>, an important model system for coral symbiosis. Lipid extracts from <i>A. pallida</i> in and out of symbiosis with its symbiont <i>Symbiodinium</i> were prepared and analyzed using negative-ion electrospray ionization quadrupole time-of-flight mass spectrometry. Through this analysis we have identified, by exact mass and collision-induced dissociation mass spectrometry (MS/MS), several classes of glycerophospholipids in <i>A. pallida</i>. Several molecular species of di-acyl phosphatidylinositol and phosphatidylserine as well as 1-alkyl, 2-acyl phosphatidylethanolamine (PE) and phosphatidycholine were identified. The 1-alkyl, 2-acyl PEs are acid sensitive suggestive that they are plasmalogen PEs possessing a double bond at the 1-position of the alkyl linked chain. In addition, we identified several molecular species of phosphonosphingolipids called ceramide aminoethylphosphonates in anemone lipid extracts by the release of a characteristic negative product ion at <i>m/z</i> 124.014 during MS/MS analysis. Sulfoquinovosyldiacylglycerol (SQDG), an anionic lipid often found in photosynthetic organisms, was identified as a prominent component of <i>Symbiodinium</i> lipid extracts. A comparison of anemone lipid profiles revealed a subset of lipids that show dramatic differences in abundance when anemones are in the symbiotic state as compared to the non-symbiotic state. The data generated in this analysis will serve as a resource to further investigate the role of lipids in symbiosis between <i>Symbiodinium</i> and <i>A. pallida</i>.</p> </div

Alkyl-phosphatidylcholines identified in <i>A. pallida</i>.

<p>Panel A shows the EIC of the [M+H]⁺ ion at <i>m/z</i> 770.6. Panel B shows the positive-ion mass spectrum from <i>m/z</i> 700 to 860 for the lipids eluting between 28.9 and 30.1 Minutes. Panel C shows the positive-ion MS/MS spectrum. The aPC predicted from this spectrum and the exact mass is shown with selected product ions indicated. Panel D shows the negative-ion MS/MS spectrum with the major product ions are indicated on the aPC structure shown below.</p

Phosphonosphingolipid species identified in <i>A. pallida</i> lipid extracts.

*<p>In the ceramide portion.</p