Hypoosmolarity influences the activity of transcription factor NF-κB in rat H4IIE hepatoma cells

AbstractThe influence of anisoosmolarity on NF-κB binding activity was studied in H4IIE rat hepatoma cells. Hypoosmolarity induced a sustained NF-κB binding activity whereas the hyperosmotic NF-κB response was only minor. Hypoosmotic NF-κB activation was accompanied by degradation of the inhibitory IκB-α. Protein kinase C, PI3-kinase, reactive oxygen intermediates and the proteasome apparently participate in mediating the hypoosmotic effect on NF-κB. Hypoosmolarity plus PMA induced, amplified and prolonged IκB-α degradation and NF-κB binding activity. Transforming growth factor β-induced apoptosis was diminished by hypoosmolarity. However, this anti-apoptotic effect was probably not related to NF-κB activation

Artificial pancreas systems for people with type 2 diabetes: Conception and design of the european CLOSE project

In the last 10 years tremendous progress has been made in the development of artificial pancreas (AP) systems for people with type 1 diabetes (T1D). The pan-European consortium CLOSE (Automated Glucose Control at Home for People with Chronic Disease) is aiming to develop integrated AP solutions (APplus) tailored to the needs of people with type 2 diabetes (T2D). APplus comprises a product and service package complementing the AP system by obligatory training as well as home visits and telemedical consultations on demand. Outcome predictors and performance indicators shall help to identify people who could benefit most from AP usage and facilitate the measurement of AP impact in diabetes care. In a first step CLOSE will establish a scalable APplus model case working at the interface between patients, homecare service providers, and payers in France. CLOSE will then scale up APplus by pursuing geographic distribution, targeting additional audiences, and enhancing AP functionalities and interconnectedness. By being part of the European Institute of Innovation and Technology (EIT) Health public-private partnership, CLOSE is committed to the EIT “knowledge triangle” pursuing the integrated advancement of technology, education, and business creation. Putting stakeholders, education, and impact into the center of APplus advancement is considered key for achieving wide AP use in T2D care