Flooding Induced Seismicity in the Ruhr Area – a geomechanics numerical modelling approach

The Ruhr region is characterized by centuries of coal mining at depths reaching more than 1000 meters. After the closure of the last mines, their controlled flooding started. The Floodrisk project investigates ground uplift, stress changes due to pore pressure changes and the reactivation potential of faults to explain induced seismicity. We focused on monitoring the eastern Ruhr area and are investigating in detail the relationship between mine water rise, tectonic stress and induced seismicity in the Haus Aden drainage area. In the region of the former "Bergwerk Ost", which had the highest seismicity in the Ruhr area during active mining, the RUB has installed a network of up to 30 short-period seismic stations. Continuous monitoring of seismicity and mine water levels is available for this region from the active mining phase through the post-mining phase to flooding. The temporal evolution of the mine water level after the pumps were shut down in mid-2019 shows a strong correlation with the temporal evolution of the observed microseismicity. Over 2200 induced events have been located since the beginning of flooding, showing spatial clustering. A comparison of the mine galleries, which today serve as the main underground waterways, with the localizations of the events shows that most of the events occur about 300 m below the main pillars located between the longwall panels. This study provides a compilation of the regional stress state in the eastern Ruhr area based on the mine measurements, which were re-evaluated to derive the regional stress component and compared with stress orientations from independent sources (information on stresses in deep boreholes and earthquake focal mechanisms). The spatial distribution of stress orientations in the Ruhr region shows a rather homogeneous stress pattern with only very few locations where stress orientations differ significantly from the average. Based on the geometry of the pillars, shafts and longwall panels, a generic numerical FE-model was developed using the compiled stress data for model calibration. The results indicate increased vertical stresses within and below the pillars as a result of stress arching. The horizontal stress changes are minor, thus differential stress increases in the vicinity of the event localization

Depletion-induced seismicity in NW-Germany: lessons from comprehensive investigations

Evaluating various investigations for north-German gas fields, we discuss past and actual evolutions of the rock fabric in the light of dilatant driven and spontaneous contractant critical phenomena. Features of the latter were discovered by multi-stage triaxial tests with water-saturated sandstone samples and were similarly observed around the gas fields. A Mohr–Coulomb condition with quasi-local stress components (${\hat{\sigma }}\u27_1$ and ${\hat{\sigma }}\u27_3$), and variable parameters ϕ’ and ${{\hat{c}}}\u27$, can capture successive critical states of the solid fabric. The implied driven dilatation up to a collapse with contraction is captured by a stress-dilatancy relation. Fractal patterns of shear bands (faults) dominate if the smallest principal stress ${\hat{\sigma }}\u27_3$ exceeds ${{\hat{c}}}\u27$, otherwise cracks dominate and can lead to a rockburst. Triaxial tests with X-ray attenuation, seismometry including the splitting of shear waves and/or neutron beam diffraction contribute to clarification and validation. Seismic early warning and calculation models for various geotechnical operations with dominating faults can thus be improved, but the task is more difficult for rockbursts

A proteomic atlas of senescence-associated secretomes for aging biomarker development.

The senescence-associated secretory phenotype (SASP) has recently emerged as a driver of and promising therapeutic target for multiple age-related conditions, ranging from neurodegeneration to cancer. The complexity of the SASP, typically assessed by a few dozen secreted proteins, has been greatly underestimated, and a small set of factors cannot explain the diverse phenotypes it produces in vivo. Here, we present the "SASP Atlas," a comprehensive proteomic database of soluble proteins and exosomal cargo SASP factors originating from multiple senescence inducers and cell types. Each profile consists of hundreds of largely distinct proteins but also includes a subset of proteins elevated in all SASPs. Our analyses identify several candidate biomarkers of cellular senescence that overlap with aging markers in human plasma, including Growth/differentiation factor 15 (GDF15), stanniocalcin 1 (STC1), and serine protease inhibitors (SERPINs), which significantly correlated with age in plasma from a human cohort, the Baltimore Longitudinal Study of Aging (BLSA). Our findings will facilitate the identification of proteins characteristic of senescence-associated phenotypes and catalog potential senescence biomarkers to assess the burden, originating stimulus, and tissue of origin of senescent cells in vivo

The analysis of slip tendency of major tectonic faults in Germany

Seismic hazard during subsurface operations is often related to the reactivation of pre-existing tectonic faults. The analysis of the slip tendency, i.e., the ratio of shear to normal stress acting on the fault plane, allows an assessment of the reactivation potential of faults. We use the total stresses that result from a large-scale 3D geomechanical–numerical model of Germany and adjacent areas to calculate the slip tendency for three 3D fault geometry sets with increasing complexity. This allows us to draw general conclusions about the influence of the fault geometry on the reactivation potential. In general, the fault reactivation potential is higher in Germany for faults that strike NW–SE and NNE–SSW. Due to the prevailing normal stress regime in the geomechanical–numerical model results, faults dipping at an angle of about 60∘ generally show higher slip tendencies in comparison to steeper or shallower dipping faults. Faults implemented with a straight geometry show higher slip tendencies than those represented with a more complex, uneven geometry. Pore pressure has been assumed to be hydrostatic and has been shown to have a major influence on the calculated slip tendencies. Compared to slip tendency values calculated without pore pressure, the consideration of pore pressure leads to an increase in slip tendency of up to 50 %. The qualitative comparison of the slip tendency with the occurrence of seismic events with moment magnitudes M$_w$>3.5 shows areas with an overall good spatial correlation between elevated slip tendencies and seismic activity but also highlights areas where more detailed and diverse fault sets would be beneficial

Glutaminyl cyclase contributes to the formation of focal and diffuse pyroglutamate (pGlu)-Aβ deposits in hippocampus via distinct cellular mechanisms

In the hippocampal formation of Alzheimer’s disease (AD) patients, both focal and diffuse deposits of Aβ peptides appear in a subregion- and layer-specific manner. Recently, pyroglutamate (pGlu or pE)-modified Aβ peptides were identified as a highly pathogenic and seeding Aβ peptide species. Since the pE modification is catalyzed by glutaminyl cyclase (QC) this enzyme emerged as a novel pharmacological target for AD therapy. Here, we reveal the role of QC in the formation of different types of hippocampal pE-Aβ aggregates. First, we demonstrate that both, focal and diffuse pE-Aβ deposits are present in defined layers of the AD hippocampus. While the focal type of pE-Aβ aggregates was found to be associated with the somata of QC-expressing interneurons, the diffuse type was not. To address this discrepancy, the hippocampus of amyloid precursor protein transgenic mice was analysed. Similar to observations made in AD, focal (i.e. core-containing) pE-Aβ deposits originating from QC-positive neurons and diffuse pE-Aβ deposits not associated with QC were detected in Tg2576 mouse hippocampus. The hippocampal layers harbouring diffuse pE-Aβ deposits receive multiple afferents from QC-rich neuronal populations of the entorhinal cortex and locus coeruleus. This might point towards a mechanism in which pE-Aβ and/or QC are being released from projection neurons at hippocampal synapses. Indeed, there are a number of reports demonstrating the reduction of diffuse, but not of focal, Aβ deposits in hippocampus after deafferentation experiments. Moreover, we demonstrate in neurons by live cell imaging and by enzymatic activity assays that QC is secreted in a constitutive and regulated manner. Thus, it is concluded that hippocampal pE-Aβ plaques may develop through at least two different mechanisms: intracellularly at sites of somatic QC activity as well as extracellularly through seeding at terminal fields of QC expressing projection neurons