Patterns of remnant discrete symmetries

We analyze patterns of remnant discrete symmetries that arise from U(1)^N theories by spontaneous breaking. We describe a simple, geometrical way to understand these patterns and provide methods for identifying the discrete symmetries and bringing them to the simplest possible form. Applications in GUT and string model building are briefly discussed.Comment: 14 pages, 2 figures, a related Mathematica package can be downloaded from http://einrichtungen.physik.tu-muenchen.de/T30e/codes/DiscreteBreaking

Heterogeneity in the developmental potential of motor neuron progenitors revealed by clonal analysis of single cells in vitro

<p>Abstract</p> <p>Background</p> <p>The differentiation of neural progenitors into distinct classes within the central nervous system occurs over an extended period during which cells become progressively restricted in their fates. In the developing spinal cord, Sonic Hedgehog (Shh) controls neural fates in a concentration-dependent manner by establishing discrete ventral progenitor domains characterized by specific combinations of transcription factors. It is unclear whether motor neuron progenitors can maintain their identities when expanded <it>in vitro </it>and whether their developmental potentials are restricted when exposed to defined extracellular signals.</p> <p>Results</p> <p>We have generated mice expressing the enhanced green fluorescent protein under the control of the <it>Nkx6.1 </it>promoter, enabling fluorescence-activated cell sorting (FACS), purification and culture of individual spinal progenitors at clonal density, and analysis of their progeny. We demonstrate that cells isolated after progenitor domains are established are heterogeneous with respect to maintaining their identity after <it>in vitro </it>expansion. Most Nkx6.1⁺progenitors lose their ventral identity following several divisions in culture, whereas a small subset is able to maintain its identity. Thus, subtype-restricted progenitors from the Nkx6.1⁺region are present in the ventral spinal cord, although at a lower frequency than expected. Clones that maintain a motor neuron identity assume a transcriptional profile characteristic of thoracic motor neurons, despite some having been isolated from non-thoracic regions initially. Exposure of progenitors to Bone Morphogenetic Protein-4 induces some dorsal cell type characteristics in their progeny, revealing that lineage-restricted progenitor subtypes are not fully committed to their fates.</p> <p>Conclusion</p> <p>These findings support a model whereby continuous Shh signaling is required to maintain the identity of ventral progenitors isolated from the spinal cord, including motor neuron progenitors, after <it>in vitro </it>expansion. They also demonstrate that pre-patterned neural progenitors isolated from the central nervous system can change their regional identity <it>in vitro </it>to acquire a broader developmental potential.</p

Running minimal flavor violation

We consider the flavor structure of the minimal supersymmetric standard model (MSSM) in the framework of `minimal flavor violation' (MFV). We show that, if one imposes the MFV structure at some scale, to a good accuracy the MFV decomposition works at all other scales. That is, quantum effects can be described by running coefficients of the MFV decomposition. We find that the coefficients get driven to non-trivial fixed points.Comment: 13 pages, 12 figure

Cooperative interactions enable singular olfactory receptor expression in mouse olfactory neurons

The monogenic and monoallelic expression of only one out of >1000 mouse olfactory receptor (ORs) genes requires the formation of large heterochromatic chromatin domains that sequester the OR gene clusters. Within these domains, intergenic transcriptional enhancers evade heterochromatic silencing and converge into interchromosomal hubs that assemble over the transcriptionally active OR. The significance of this nuclear organization in OR choice remains elusive. Here, we show that transcription factors Lhx2 and Ebf specify OR enhancers by binding in a functionally cooperative fashion to stereotypically spaced motifs that defy heterochromatin. Specific displacement of Lhx2 and Ebf from OR enhancers resulted in pervasive, long-range, and trans downregulation of OR transcription, whereas pre-assembly of a multi-enhancer hub increased the frequency of OR choice in cis. Our data provide genetic support for the requirement and sufficiency of interchromosomal interactions in singular OR choice and generate general regulatory principles for stochastic, mutually exclusive gene expression programs

Guiding cell adhesion and motility by modulating cross-linking and topographic properties of microgel arrays

Biomaterial-driven modulation of cell adhesion and migration is a challenging aspect of tissue engineering. Here, we investigated the impact of surface-bound microgel arrays with variable geometry and adjustable cross-linking properties on cell adhesion and migration. We show that cell migration is inversely correlated with microgel array spacing, whereas directionality increases as array spacing increases. Focal adhesion dynamics is also modulated by microgel topography resulting in less dynamic focal adhesions on surface-bound microgels. Microgels also modulate the motility and adhesion of Sertoli cells used as a model for cell migration and adhesion. Both focal adhesion dynamics and speed are reduced on microgels. Interestingly, Gas2L1, a component of the cytoskeleton that mediates the interaction between microtubules and microfilaments, is dispensable for the regulation of cell adhesion and migration on microgels. Finally, increasing microgel cross-linking causes a clear reduction of focal adhesion turnover in Sertoli cells. These findings not only show that spacing and rigidity of surface-grafted microgels arrays can be effectively used to modulate cell adhesion and motility of diverse cellular systems, but they also form the basis for future developments in the fields of medicine and tissue engineering