26 research outputs found

    The Sabbath : in the law, in the prophets, and in Mark

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    Different versions of the Sabbath law (Exodus 20:8-11; 23:12; 31:12-17; 34:21; 35:1-3; Leviticus 23:3; Deuteronomy 5:12-15) and several prophetic texts (Amos 6:1-7 - the name "Sabbath" is text-critically reconstructed in verse 3 with the help of the Septuagint); Amos 8:4-7; Ezekiel 20; Jeremiah 17:19-27; Isaiah 56:1-8) reflect a vivid struggle over the centuries as to how the Sabbath should be understood and practised. Jesus' position towards the Sabbath (Mark 2:23-28) pursues the prophetic intention: the goal is decisive. The last part states that Sabbath and Sunday contribute to a sustainable development, because they counterbalance greed and short-sightedness by self constrain and openness to God's time rhythm.http://explore.up.ac.za/record=b152516

    Triple Orthogonal Labeling of Glycans by Applying Photoclick Chemistry

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    Bioorthogonal labeling of multiple biomolecules is of current interest in chemical biology. Metabolic glycoengineering (MGE) has been shown to be an appropriate approach to visualizing carbohydrates. Here, we report that the nitrile imine–alkene cycloaddition (photoclick reaction) is a suitable ligation reaction in MGE. Using a mannosamine derivative with an acrylamide reporter group that is efficiently metabolized by cells and that quickly reacts in the photoclick reaction, we labeled sialic acids on the surface of living cells. Screening of several alkenes showed that a previously reported carbamate‐linked methylcyclopropene reporter that is well suited for the inverse‐electron‐demand Diels–Alder (DAinv) reaction has a surprisingly low reactivity in the photoclick reaction. Thus, for the first time, we were able to triply label glycans by a combination of DAinv, photoclick, and copper‐free click chemistry.publishe
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