On the representation of cells in bone marrow pathology by a scalar field: propagation through serial sections, co-localization and spatial interaction analysis

Background: Immunohistochemical analysis of cellular interactions in the bone marrow in situ is demanding, due to its heterogeneous cellular composition, the poor delineation and overlap of functional compartments and highly complex immunophenotypes of several cell populations (e.g. regulatory T-cells) that require immunohistochemical marker sets for unambiguous characterization. To overcome these difficulties, we herein present an approach to describe objects (e.g. cells, bone trabeculae) by a scalar field that can be propagated through registered images of serial histological sections. Methods: The transformation of objects within images (e.g. cells) to a scalar field was performed by convolution of the object’s centroids with differently formed radial basis function (e.g. for direct or indirect spatial interaction). On the basis of such a scalar field, a summation field described distributed objects within an image. Results: After image registration i) colocalization analysis could be performed on basis scalar field, which is propagated through registered images, and - due to the shape of the field – were barely prone to matching errors and morphological changes by different cutting levels; ii) furthermore, depending on the field shape the colocalization measurements could also quantify spatial interaction (e.g. direct or paracrine cellular contact); ii) the field-overlap, which represents the spatial distance, of different objects (e.g. two cells) could be calculated by the histogram intersection. Conclusions: The description of objects (e.g. cells, cell clusters, bone trabeculae etc.) as a field offers several possibilities: First, co-localization of different markers (e.g. by immunohistochemical staining) in serial sections can be performed in an automatic, objective and quantifiable way. In contrast to multicolour staining (e.g. 10-colour immunofluorescence) the financial and technical requirements are fairly minor. Second, the approach allows searching for different types of spatial interactions (e.g. direct and indirect cellular interaction) between objects by taking field shape into account (e.g. thin vs. broad). Third, by describing spatially distributed groups of objects as summation field, it gives cluster definition that relies rather on the bare object distance than on the modelled spatial cellular interaction

On the representation of cells in bone marrow pathology by a scalar field: propagation through serial sections, co-localization and spatial interaction analysis

Background Immunohistochemical analysis of cellular interactions in the bone marrow in situ is demanding, due to its heterogeneous cellular composition, the poor delineation and overlap of functional compartments and highly complex immunophenotypes of several cell populations (e.g. regulatory T-cells) that require immunohistochemical marker sets for unambiguous characterization. To overcome these difficulties, we herein present an approach to describe objects (e.g. cells, bone trabeculae) by a scalar field that can be propagated through registered images of serial histological sections. Methods The transformation of objects within images (e.g. cells) to a scalar field was performed by convolution of the object’s centroids with differently formed radial basis function (e.g. for direct or indirect spatial interaction). On the basis of such a scalar field, a summation field described distributed objects within an image. Results After image registration i) colocalization analysis could be performed on basis scalar field, which is propagated through registered images, and - due to the shape of the field – were barely prone to matching errors and morphological changes by different cutting levels; ii) furthermore, depending on the field shape the colocalization measurements could also quantify spatial interaction (e.g. direct or paracrine cellular contact); ii) the field-overlap, which represents the spatial distance, of different objects (e.g. two cells) could be calculated by the histogram intersection. Conclusions The description of objects (e.g. cells, cell clusters, bone trabeculae etc.) as a field offers several possibilities: First, co-localization of different markers (e.g. by immunohistochemical staining) in serial sections can be performed in an automatic, objective and quantifiable way. In contrast to multicolour staining (e.g. 10-colour immunofluorescence) the financial and technical requirements are fairly minor. Second, the approach allows searching for different types of spatial interactions (e.g. direct and indirect cellular interaction) between objects by taking field shape into account (e.g. thin vs. broad). Third, by describing spatially distributed groups of objects as summation field, it gives cluster definition that relies rather on the bare object distance than on the modelled spatial cellular interaction

Die glückseligste Veränderung Wolte bey dem Grabe der weiland Hoch-Edlen, Hoch-Ehr und Tugendbelobten Frauen, Frauen Annen Margarethen Zinckin geb. Bittstedtin, des ... Herrn Johann Adam Zincken, Treu-verdienten Pfarrers und Adjuncti der Christlichen Gemeinde in Frauenbreitungen ... nachgelassenen Frau Wittwen, als seiner ... Frau Schwieger-Mutter, als solche ... den 7ten Augusti in Römhild durch einen seligen Tod von Jhm getrennet ward mitleidigst erwegen ... Johann Christoph Scharff, Superint. Römhild ...

Autopsie nach Exemplar der ULB Sachsen-AnhaltVorlageform des Erscheinungsvermerks: Römhild, druckts Johann Georg Brückner. - Erscheinungsjahr nach Todes jahr der Anna Margarethe Zinck bestimm

Die Ethik politischer Kommunikation im franko-burgundischen Spätmittelalter

Schulte P. Die Ethik politischer Kommunikation im franko-burgundischen Spätmittelalter. In: Dartmann C, Scharff T, Weber CF, eds. Zwischen Pragmatik und Performanz: Dimensionen mittelalterlicher Schriftkultur. Utrecht studies in medieval literacy. Vol 18. Turnhout: Brepols; 2011: 461-489

Petrus Abaelardus als Kronzeuge der "Individualität" im 12. Jahrhundert? Einige Fragen

Arlinghaus F-J. Petrus Abaelardus als Kronzeuge der "Individualität" im 12. Jahrhundert? Einige Fragen. In: Dartmann C, Scharff T, Weber CF, eds. Zwischen Pragmatik und Performanz: Dimensionen mittelalterlicher Schriftkultur. Utrecht Studies in Medieval Literacy. Vol 18. Turnhout: Brepols; 2011: 165-197

Betrübte Worte bey Des weyland Hoch-Ehrwürdigen, in Gott Andächtigen und Hochgelahrten Herrn Joh. Adam Zinckens, Best-meritirt gewesenen treuen Pastoris und Adivncti ... in Frauenbreitungen, Seelig erfolgten Ableben den 8ten Martii 1729. .... Jn ihrem grösten Kummer geführet von Johann Christoph Scharffen, Past. zu Steinbach, Ernst David Nattermann, Past. zu Ober-Ella

Gedächtnisgedicht auf Johann Adam Zinck, Pastor, +8. März 1729Autopsie nach Exemplar der ULB Sachsen-AnhaltVorlageform des Erscheinungsvermerks: Meiningen, druckts Jonas Christoph Hassert, Fürstl. Sächß. Hof-Buchdr

Aprotinin Prevents the Development of the Trauma-Induced Multiple Organ Failure in a Chronic Sheep Model

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