230 research outputs found
Correlation plenoptic imaging
Plenoptic imaging is a promising optical modality that simultaneously
captures the location and the propagation direction of light in order to enable
three-dimensional imaging in a single shot. However, in classical imaging
systems, the maximum spatial and angular resolutions are fundamentally linked;
thereby, the maximum achievable depth of field is inversely proportional to the
spatial resolution. We propose to take advantage of the second-order
correlation properties of light to overcome this fundamental limitation. In
this paper, we demonstrate that the momentum/position correlation of chaotic
light leads to the enhanced refocusing power of correlation plenoptic imaging
with respect to standard plenoptic imaging.Comment: 6 pages, 3 figure
Exploring plenoptic properties of correlation imaging with chaotic light
In a setup illuminated by chaotic light, we consider different schemes that
enable to perform imaging by measuring second-order intensity correlations. The
most relevant feature of the proposed protocols is the ability to perform
plenoptic imaging, namely to reconstruct the geometrical path of light
propagating in the system, by imaging both the object and the focusing element.
This property allows to encode, in a single data acquisition, both
multi-perspective images of the scene and light distribution in different
planes between the scene and the focusing element. We unveil the plenoptic
property of three different setups, explore their refocusing potentialities and
discuss their practical applications.Comment: 9 pages, 4 figure
Correlation Plenoptic Imaging With Entangled Photons
Plenoptic imaging is a novel optical technique for three-dimensional imaging
in a single shot. It is enabled by the simultaneous measurement of both the
location and the propagation direction of light in a given scene. In the
standard approach, the maximum spatial and angular resolutions are inversely
proportional, and so are the resolution and the maximum achievable depth of
focus of the 3D image. We have recently proposed a method to overcome such
fundamental limits by combining plenoptic imaging with an intriguing
correlation remote-imaging technique: ghost imaging. Here, we theoretically
demonstrate that correlation plenoptic imaging can be effectively achieved by
exploiting the position-momentum entanglement characterizing spontaneous
parametric down-conversion (SPDC) photon pairs. As a proof-of-principle
demonstration, we shall show that correlation plenoptic imaging with entangled
photons may enable the refocusing of an out-of-focus image at the same depth of
focus of a standard plenoptic device, but without sacrificing
diffraction-limited image resolution.Comment: 12 pages, 5 figure
Diffraction-limited plenoptic imaging with correlated light
Traditional optical imaging faces an unavoidable trade-off between resolution
and depth of field (DOF). To increase resolution, high numerical apertures (NA)
are needed, but the associated large angular uncertainty results in a limited
range of depths that can be put in sharp focus. Plenoptic imaging was
introduced a few years ago to remedy this trade off. To this aim, plenoptic
imaging reconstructs the path of light rays from the lens to the sensor.
However, the improvement offered by standard plenoptic imaging is practical and
not fundamental: the increased DOF leads to a proportional reduction of the
resolution well above the diffraction limit imposed by the lens NA. In this
paper, we demonstrate that correlation measurements enable pushing plenoptic
imaging to its fundamental limits of both resolution and DOF. Namely, we
demonstrate to maintain the imaging resolution at the diffraction limit while
increasing the depth of field by a factor of 7. Our results represent the
theoretical and experimental basis for the effective development of the
promising applications of plenoptic imaging.Comment: 10 pages, 10 figure
Biomechanical properties of murine embryos using optical coherence tomography and brilloiun microscopy
A combination of optical coherence tomograph
Direct 3D imaging through spatial coherence of light
Wide-field imaging is widely adopted due to its fast acquisition,
cost-effectiveness and ease of use. Its extension to direct volumetric
applications, however, is burdened by the trade-off between resolution and
depth of field (DOF), dictated by the numerical aperture of the system. We
demonstrate that such trade-off is not intrinsic to wide-field imaging, but
stems from the spatial incoherence of light: images obtained through spatially
coherent illumination are shown to have resolution and DOF independent of the
numerical aperture. This fundamental discovery enabled us to demonstrate an
optimal combination of coherent resolution-DOF enhancement and incoherent
tomographic sectioning for scanning-free, wide-field 3D microscopy on a
multicolor histological section.Comment: 17 pages, 6 figures. Supplemental document available upon request to
the authors. Submitted to Lasers and Photonics Review
An RbAp48-like gene regulates adult stem cells in planarians.
Retinoblastoma-associated proteins 46 and 48 (RbAp46 and RbAp48) are factors that are components of different chromatin-modelling complexes, such as polycomb repressive complex 2, the activity of which is related to epigenetic gene regulation in stem cells. To date, no direct findings are available on the in vivo role of RbAp48 in stem-cell biology. We recently identified DjRbAp48 — a planarian ( Dugesia japonica ) homologue of human RBAP48 — expression of which is restricted to the neoblasts, the adult stem cells of planarians. In vivo silencing of DjRbAp48 induces lethality and inability to regenerate, even though neoblasts proliferate and accumulate after wounding. Despite a partial reduction in neoblast number, we were always able to detect a significant number of these cells in DjRbAp48 RNAi animals. Parallel to the decrease in neoblasts, a reduction in the number of differentiated cells and the presence of apoptotic-like neoblasts were detectable in RNAi animals. These findings suggest that DjRbAp48 is not involved in neoblast maintenance, but rather in the regulation of differentiation of stem-cell progeny. We discuss our data, taking into account the possibility that DjRbAp48 might control the expression of genes necessary for cell differentiation by influencing chromatin architecture
Tumor cell nuclei soften during transendothelial migration
During cancer metastasis, tumor cells undergo significant deformation in order to traverse through endothelial cell junctions in the walls of blood vessels. As cells pass through narrow gaps, smaller than the nuclear diameter, the spatial configuration of chromatin must change along with the distribution of nuclear enzymes. Nuclear stiffness is an important determinant of the ability of cells to undergo transendothelial migration, yet no studies have been conducted to assess whether tumor cell cytoskeletal or nuclear stiffness changes during this critical process in order to facilitate passage. To address this question, we employed two non-contact methods, Brillouin confocal microscopy (BCM) and confocal reflectance quantitative phase microscopy (QPM), to track the changes in mechanical properties of live, transmigrating tumor cells in an in vitro collagen gel platform. Using these two imaging modalities to study transmigrating MDA-MB-231, A549, and A375 cells, we found that both the cells and their nuclei soften upon extravasation and that the nuclear membranes remain soft for at least 24 h. These new data suggest that tumor cells adjust their mechanical properties in order to facilitate extravasation
Bisphenol A and Bisphenol S Induce Endocrine and Chromosomal Alterations in Brown Trout
Bisphenol A is a widely used compound found in large amount of consumer products. As concerns have been raised about its toxicological and public health effect, the use of alternatives to bisphenol A are now increasing. Bisphenol S is one of the analogues being used as a replacement for bisphenol A despite the fact that little is known about the effects of bisphenol S on living organisms. In this study, we investigated the potential endocrine and genotoxic effects of bisphenol A and bisphenol S in juvenile brown trout (Salmo trutta). The fish were exposed to the compounds for either 2 weeks or 8 weeks via sustained-release cholesterol implants containing doses of 2 mg/kg fish or 20 mg/kg fish of the substances. The effects on the thyroid hormone levels and the estrogenic disrupting marker vitellogenin were evaluated, along with the genotoxic markers micronucleated cells and erythrocyte nuclear abnormalities. An increase in plasma vitellogenin was observed in fish exposed to the high dose of bisphenol A for 2 weeks. At this experimental time the level of the thyroid hormone triiodothyronine (T3) in plasma was elevated after bisphenol S exposure at the high concentration, and paralleled by an increase of micronucleated cells. Moreover, bisphenol A induced an increase of micronuclei frequency in fish erythrocytes after the exposure at the lowest dose tested. Taken together the results indicate that both bisphenol A and its alternative bisphenol S cause endocrine disrupting and genotoxic effects in brown trout, although suggesting two different mechanisms of damage underlying bisphenol A and bisphenol S activity
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