Genomic view of selected genes for comparison between manual and automation methods.

<p>CAGE mapped read counts were displayed as linear scale histogram on ACTB (A) and GAPDH (B) genes. The transcription initiation regions are magnified to demonstrate the tag density distribution is consistent between manual and automated libraries. Green and purple indicate plus and minus strands, respectively.</p

Representative scatter plots demonstrating reproducibility between manual and automated workflows and reproducibility within batches and between batches using automation.

<p>A–C: shows scatterplots of TPM normalized gene expression for A, two technical replicates in the same batch; B: between technical replicates from different batches and; C: between manually and automatically prepared technical replicates. Finally D: shows the average correlation coefficient when comparing multiple replicates from automation and manual libraries. Error bars indicate the standard deviation.</p

Evaluation of replicate THP-1 CAGE libraries.

<p>The quality control values for all replicate THP-1 CAGE libraries and sequencing/mapping evaluation are listed. All values are averages with standard deviations. The Ct values of ACTB and 18S rRNA are representatives of non-capped and capped transcripts. The primers were designed at near 5′ end of each transcript. The promoter ratio and rRNA ratio are the rates of reads mapped at 5′ end per total filtered reads. The detail information is shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0030809#pone.0030809.s005" target="_blank">Table S2</a>.</p

Collision detection on transmission lines with optical interferometer

V diplomski nalogi skušamo ugotoviti, v kolikšni meri je možno zaznavati in klasificirati trke na jeklenicah daljnovodov z optičnim interferometrom. Na začetku predstavimo osnovne pojme interferometrije in opišemo uporabljen optični interferometer. V jedru diplomske naloge natančneje opišemo eksperimentalni protokol in obdelavo signalov. Nadaljujemo z implementacijo algoritmov za segmentacijo in klasifikacijo zajetih signalov ter predstavimo dobljene rezultate. Segmentacijo izvedemo v domeni števila prehodov signala skozi ničlo, za klasifikacijo pa uporabimo večplastno nevronsko mrežo z algoritmom vzvratnega učenja. Rezultati študije nakazujejo, da sta implementirani segmentacija in klasifikacija uspešni v 77 % izvedenih trkov različnih predmetov.We analyse feasibility of collision detection on transmission lines with optical interferometer. We first provide a brief introduction into interferometry, along with a description of the optical interferometer used for measurements in this study. Afterwards, we describe the conducted experimental protocol and signal processing methodology. The focus is on implementation of algorithms for signal segmentation and collision classification. We used zero-crossing algorithm to transform signals into segmentation domain. Classification of collisions is done with a multilayer neural network trained by the backpropagation algorithm. The results demonstrate an average success rate of 77% for segmentation and classification of collision with five different objects

Sistematización de la experiencia de un ambiente de aprendizaje enriquecido por TIC durante la práctica clínica en fisioterapia cardiopulmonar en un hospital de nivel II de la ciudad de Cali

Esta investigación se centra en la caracterización de la experiencia de 4 estudiantes de fisioterapia de IX semestre de la Institución Universitaria Escuela Nacional del Deporte (IUEND) durante la implementación de un ambiente de aprendizaje enriquecido con Tecnologías de la Información y la Comunicación (TIC) en la práctica clínico – asistencial en Salud Cardiopulmonar; la cual se fundamenta en el hacer y pone a prueba las bases conceptuales del ciclo de fundamentación; todo esto con el fin de identificar las experiencias significativas que facilitan el aprendizaje y desarrollo de competencias clínicas, además analizar si este tipo de estrategias de enseñanza -aprendizaje permite al estudiante y al docente asesor superar inconvenientes propios de la práctica clínica como: optimizar tiempos de atención a pacientes, estudio independiente y trabajo colaborativo, retomar e integrar gran cantidad de conceptos y procedimientos aprendidos en IV semestre con las nuevas experiencias y la realidad del paciente; y a la vez cumplir con funciones administrativas propios del rol del fisioterapeuta asistencial (estadística, indicadores, desarrollo de guías, etc.) que dificultan el proceso de aprendizaje; concluyendo que los ambientes mediados por TIC pueden lograr superar estas dificultades y favorecer finalmente el aprendizaje significativo (juicio clínico), en el que se fundamenta el ciclo de práctica profesional

assay information of the mouse tissue samples for HeliScopeCAGE

a tab delimited flat file (SDRF file) describing the experimental details for mouse tissue samples for the standard protocol of the HeliScopeCAGE protoco

assay information of the mouse qualitycontrol samples for HeliScopeCAGE

a tab delimited flat file (SDRF file) describing the experimental details for mouse quality control samples for the standard protocol of the HeliScopeCAGE protoco

DRA002216

List of DRA (http://trace.ddbj.nig.ac.jp/dra/index_e.html) accession numbers of the FANTOM5 samples, sequences and genomic coordinations. Files are in tab-delimited format, which includes * Library ID * FFID * BioSamples accession number * DRA experiment accession number * DRA run accession numbers * DRA analysis accession number for genomic coordination (BAM file) * DRA analysis accession number for CTSS (BED file) * Experiment method (CAGE/RNA-Seq/sRNA-Seq

assay information of the unclassified samples for HeliScopeCAGE

a tab delimited flat file (SDRF file) describing the experimental details for unclassified samples for the standard protocol of the HeliScopeCAGE protoco

DRA001028

List of DRA (http://trace.ddbj.nig.ac.jp/dra/index_e.html) accession numbers of the FANTOM5 samples, sequences and genomic coordinations. Files are in tab-delimited format, which includes * Library ID * FFID * BioSamples accession number * DRA experiment accession number * DRA run accession numbers * DRA analysis accession number for genomic coordination (BAM file) * DRA analysis accession number for CTSS (BED file) * Experiment method (CAGE/RNA-Seq/sRNA-Seq