Extraction, Partial Characterization And Nutritional Aspects Of Proteins From Carioca 80 Bean (phaseolus Vulgaris L.) [extração, Caracterização Parcial E Aspectos Nutricionais Das Proteínas Do Feijão Carioca 80 (phaseolus Vulgaris L.).]

Extraction, fractionation and characterization by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis of proteins from Carioca 80 beans (Phaseolus vulgaris) were performed at three pH values (2.5, 8.0 and 9.0). Extraction at pH 7.0 proved to be more efficient and, after dialysis, produced a better separation of the albumin and globulin fractions. Relative mobility of the main protein in the globulin fractions occurred between 0.30 and 0.40, and dissociation was observed when the pH was increased. The two most representative bands gave molecular weights of 35,400 and 76,900, while in regard to the trypsin inhibitor, three bands gave 28,800, 22,500 and 18,300. Pepsin and pancreatin in vitro digestibility rendered values of 33.43% and 62.63% for whole flour and for protein precipitated at pH 4.5, respectively. The content of available methionine found, of 1.36 g/16 g N, appears to be high in relation to that of other bean varieties.3718810

Variabilidade isoenzimática em oito raças de milho Isoenzyme variation among eight races of maize

Com o objetivo de avaliar a variabilidade genética e as relações de afinidade entre dezenove populações de oito raças de milho (Zea mays L.) - as comerciais antigas Cateto Sulino, Cateto Sulino Grosso, Cateto Nortista e Canario de Ocho, e as raças indígenas Moroti, Lenha, Entrelaçado e Caingang - analisaram-se os seguintes sistemas enzimáticos: glutamato oxalacetato transaminase (GOT), esterase (EST) e malato desidrogenase (MDH). Observou-se maior semelhança entre as raças pertencentes a um mesmo grupo, mas as populações analisadas não se agruparam de acordo com as raças, classificadas anteriormente segundo caracteres morfológicos. Os sistemas enzimáticos utilizados não permitiram a caracterização individual de cada uma das raças analisadas. As indígenas apresentaram maior variabilidade do que as comerciais antigas quanto ao número de alelos por loco e à porcentagem de locos polimórficos.<br>The objective of this study was to evaluate the genetic variability and affinity relationships among eight races of maize (Zea mays L.): four ancient varieties (Cateto Sulino, Cateto Sulino Grosso, Cateto Nortista and Canario de Ocho) and four indigenous (Moroti, Lenha, Entrelaçado and Caingang), through isoenzymatic polymorphisms. The following isoenzymatic systems were evaluated: Glutamate Oxaloacetate Transaminase (GOT), Esterase (EST) and Malate Dehydrogenase (MDH). It was observed a higher identity among races belonging to the same racial group; however, populations within each race were not grouped according, to previous morphological classification. Indigenous races showed higher average number of alleles per loci and percentage of polymorphic loci than the ancient varieties. This fact might be due to the selection the ancient varieties had been gone through