EVOLUTION OF NANOTECH ASSISTED PCR DIAGNOSIS OF MYCOBACTERIUM TUBERCULOSIS AND ITS ASSESSMENT WITH CONVENTIONAL METHODS

Objective: This study was focused on assessment and performance of conventional and nanotech assisted methods for detection of Mycobacterium tuberculosis. Tuberculosis (TB) still leftovers one of the top ten causes of death worldwide, thus this study has undertaken the use of MNP for early detection of TB.Methods: A cross-sectional studies were conducted on clinical and radiological suspected TB patients in the department of microbiology at D. Y. Patil hospital Kolhapur. All samples received in the month of August 2016 to January 2017. Total One hundred twenty-sputum samples were processed for diagnosis of TB by ZN stain, culture on L. J. Medium and real-timePCR tests.Results: In our present study, 60.83% (73) patient male and 39.17 % (47) were female patient, showing a borderline male prevalence found in our study. Eighteen percent patients were found to be in the age group (21-30 y) are mostly affected for pulmonary tuberculosis. A significant difference was seen in the percentage of differentDNA extraction methods, the fig. being conventional chloroform-phenol 66.66%, the commercial kit 80%, magnetic bead 86.66% and MNPs method 99.66% found to be significant (P<0.0001**).Conclusion: In the present study the MNP-DNA extraction techniques with NALC followed by IS6110 target amplification were found superior for diagnosis of TB. The MNP assisted extraction method showed better results in terms of quantification and sensitivity of TB PCR diagnosis, evolving nanotech assisted innovative method

Electron acceleration in a post-flare decimetric continuum source

Aims: To calculate the power budget for electron acceleration and the efficiency of the plasma emission mechanism in a post-flare decimetric continuum source. Methods: We have imaged a high brightness temperature ($\sim 10^{9}$K) post-flare source at 1060 MHz with the Giant Metrewave Radio Telescope (GMRT). We use information from these images and the dynamic spectrum from the Hiraiso spectrograph together with the theoretical method described in Subramanian & Becker (2006) to calculate the power input to the electron acceleration process. The method assumes that the electrons are accelerated via a second-order Fermi acceleration mechanism. Results: We find that the power input to the nonthermal electrons is in the range $3\times 10^{25}$--$10^{26}$ erg/s. The efficiency of the overall plasma emission process starting from electron acceleration and culminating in the observed emission could range from $2.87\times 10^{-9}$ to $2.38 \times 10^{-8}$.Comment: Accepted for publication, Astronomy and Astrophysic

VIBRATIONAL ANALYSIS OF QUARTER CAR VEHICLE DYNAMIC SYSTEM SUBJECTED TO HARMONIC EXCITATION BY ROAD SURFACE

A front suspension of Hyundai Elantra 1992 model is assigned as quarter car model and is considered for the performance study. Modeling the dynamic performance of an automobile car system represents a complex task and forms an important step in its design procedure. In this paper the stationary response of quarter car vehicle model moving with a constant velocity over a rough road is considered for the performance study. For this a simplified model and experimental set up is developed. The deterministic impulses due to road profile are given by an eccentric cam which gives input motion to front suspension acting as a follower of the cam. The displacements obtained by FFT analyzer at upper mount of shock absorber were compared with the analytical and MATLAB results

Complementary bursts, coronal inhomogeneities and new microscopic spectral features of solar bursts in type IV bursts

Complementary bursts (C.B's) have been observed in the decametric range during noise storms and/or type IV activity. These bursts essentially consist of two components, each component having a duration ~ 1 second. The first component shows weak emission or emission gap over a certain frequency range. The second component is observed after a certain delay. If the bursts are assumed to be generated at the fundamental, and if the radiation corresponding to the gap propagates through an electron density irregularity located close to the source along the line of sight, whose cross-section is less than the linear extent of the source, then almost all properties of the C.B.'s can be explained. High sensitivity and high frequency and time resolution spectra of type IV bursts at 137 MHz revealed new microscopic spectral features displaying "wave- like" and "fork- like" shapes

Serum MicroRNA-21 as a Biomarker for Allergic Inflammatory Disease in Children

MicroRNAs (miRs) have emerged as useful biomarkers for different disease states, including allergic inflammatory diseases such as asthma and eosinophilic esophagitis (EoE). Serum miRs are a possible non-invasive method for diagnosis of such diseases. We focused on microRNA-21 (miR-21) levels in serum, in order to assess the feasibility of using this gene as a non-invasive biomarker for these diseases in the clinic, as well as to better understand the expression pattern of miR-21 in allergic inflammation. We used quantitative PCR (QPCR) to assay miR-21 and other control miRs in esophageal biopsies from EoE patients and serum samples from EoE and asthma patients. Serum levels of miR-21 were significantly elevated in patients with asthma, whereas serum miR-21 levels were not associated with the presence of allergen-specific IgE (i.e. atopy). Esophageal biopsies showed a large elevation of miR-21 in EoE and an increase in miR-21 in EoE serum. Control U6 miR did not vary between asthma and control patients, however EoE serum had significantly decreased U6 microRNA compared to controls. The decreased U6 in EoE sera did not completely account for the relative increase in miR-21 in the sera of EoE patients. We report for the first time that miR-21 is elevated in the sera of both asthma and EoE patients. We find no relation between serum miR-21 levels and atopy. Our results thus suggest miR-21 is a novel biomarker for human allergic inflammatory diseases

Solar Images Processing in Parallel Environment

This paper presents the PVA-BDA project (Processing, Visualization and Analysis in ParallelEnvironment of the BDA Data) that has been developed for processing of solar images that will be captured bythe BDA (Brazilian Decimetric Array), a radio telescope under development at the National Institute for SpaceResearch (INPE). In a joint effort between the Department of Computer Science at Federal University of SãoCarlos (DC/UFSCar), the Astrophysics Division (DAS) and Associated Laboratory for Computing and AppliedMathematics (LAC) at INPE, a high performance parallel system is being developed with capacity to supportrealistic applications, involving a reasonable amount of parallel processing, in order to carry out the processing,visualization and analysis of solar images captured by BDA, in real time. The aim is to create the conditions forstarting a study of the solar weather forecast. The forecast of solar explosions are important as they may causeserious perturbations in terrestrial communication systems. An application for 3D reconstruction of X-raytomographic images of the solar atmosphere was developed at DC/UFSCar. Due to the need for the 3Dreconstruction of solar magnetic structures, in real-time, this application was implemented to execute in a parallelmachine using DSPs

Sinteza i biološko vrednovanje novih derivata 2-fenil-benzimidazol-1-acetamida kao potencijalnih anthelmintika

The present study describes synthesis of a series of 2-phenyl benzimidazole-1-acetamide derivatives and their evaluation for anthelmintic activity using Indian adult earthworms, Pheretima posthuma. The structure of the title compounds was elucidated by elemental analysis and spectral data. The compounds 4-({[2-(4-nitrophenyl)-1H-benzimidazol-1-yl]acetyl}amino) benzoic acid (3a), N-ethyl-2-[2-(4-nitrophenyl)-1H-benzimidazol-1-yl] acetamide (3c), N-benzyl-2-[2-(4-nitrophenyl)-1H-benzimidazol-1-yl] acetamide (3d), N-(4-hydroxyphenyl)-2-[2-(4-nitrophenyl)-1H-benzimidazol-1-yl] acetamide (3f), 2-[2-(4-nitrophenyl)-1H-benzimidazol-1-yl]-N-phenylacetamide (3h), 2-[2-(4-chlorophenyl)-1H-benzimidazol-1-yl]-N\u27-phenylacetohydrazide (3k), 2-[2-(4-chlorophenyl)-1H-benzimidazol-1-yl]-N-(4-nitrophenyl) acetamide (3n) and 2-[2-(4-chlorophenyl)-1H-benzimidazol-1-yl]-N-phenylacetamide (3q) were better to paralyze worms whereas N-ethyl-2-[2-(4-nitrophenyl)-1H-benzimidazol-1-yl] acetamide (3c), N-(4-nitrophenyl)-2-[2-(4-nitrophenyl)-1H-benzimidazol-1yl] acetamide (3e), 4-({[2-(4-chlorophenyl)-1H-benzimidazol-1-yl] acetyl} amino) benzoic acid (3j), 2-[2-(4-chlorophenyl)-1H-benzimidazol-1-yl]-N-ethylacetamide (3l) and 2-[2-(4-chlorophenyl)-1H-benzimidazol-1-yl]-N-phenylacetamide (3q) were better to cause death of worms compared to the anthelmintic drug albendazole.U radu je opisana sinteza derivata 2-fenil-benzimidazol-1-acetamida i ispitivanje njihovog anthelmintičkog djelovanja na odrasle indijske gliste, Pheretima posthuma. Struktura sintetiziranih spojeva određena je elementarnom analizom i spektroskopskim metodama. Spojevi 4-({[2-(4-nitrofenil)-1H-benzimidazol-1-il]acetil}amino) benzojeva kiselina (3a), N-etil-2-[2-(4-nitrofenil)-1H-benzimidazol-1-il] acetamid (3c), N-benzil-2-[2-(4-nitrofenil)-1H-benzimidazol-1-il] acetamid (3d), N-(4-hidroksifenil)-2-[2-(4-nitrofenil)-1H-benzimidazol-1-il] acetamid (3f), 2-[2-(4-nitrofenil)-1H-benzimidazol-1-il]-N-fenilacetamid (3h), 2-[2-(4-klorfenil)-1H-benzimidazol-1-il]-N\u27-fenilacetohidrazid (3k), 2-[2-(4-klorfenil)-1H-benzimidazol-1-il]-N-(4-nitrofenil) acetamid (3n) i 2-[2-(4-klorfenil)-1H-benzimidazol-1-il]-N-fenilacetamid (3q) jače paraliziraju gliste, a N-etil-2-[2-(4-nitrofenil)-1H-benzimidazol-1-il] acetamid (3c), 2-[2-(4-nitrofenil)-1H-benzimidazol-1-il]-N-fenilacetamid (3h), 4-({[2-(4-klorfenil)-1H-benzimidazol-1-il]acetil}amino) benzojeva kiselina (3j), 2-[2-(4-klorfenil)-1H-benzimidazol-1-il]-N-etilacetamid (3l) i 2-[2-(4-klorfenil)-1H-benzimidazol-1-il]-N-fenilacetamid (3q) učinkovitije usmrćuju gliste nego anthelmintik albendazol