UTILLdb, a Pisum sativum in silico forward and reverse genetics tool

UTILLdb is a database of phenotypic and sequence information on mutant genes from a reference Pisum sativum EMS-mutant population

Translational Genomics in Legumes Allowed Placing In Silico 5460 Unigenes on the Pea Functional Map and Identified Candidate Genes in Pisum sativum L.

To identify genes involved in phenotypic traits, translational genomics from highly characterized model plants to poorly characterized crop plants provides a valuable source of markers to saturate a zone of interest as well as functionally characterized candidate genes. In this paper, an integrated view of the pea genetic map was developed. A series of gene markers were mapped and their best reciprocal homologs were identified on M. truncatula, L. japonicus, soybean, and poplar pseudomolecules. Based on the syntenic relationships uncovered between pea and M. truncatula, 5460 pea Unigenes were tentatively placed on the consensus map. A new bioinformatics tool, http://www.thelegumeportal.net/pea_mtr_translational_toolkit, was developed that allows, for any gene sequence, to search its putative position on the pea consensus map and hence to search for candidate genes among neighboring Unigenes. As an example, a promising candidate gene for the hypernodulation mutation nod3 in pea was proposed based on the map position of the likely homolog of Pub1, a M. truncatula gene involved in nodulation regulation. A broader view of pea genome evolution was obtained by revealing syntenic relationships between pea and sequenced genomes. Blocks of synteny were identified which gave new insights into the evolution of chromosome structure in Papillionoids and Eudicots. The power of the translational genomics approach was underlined

Mieux connaître les processus de prises de décision des praticiens pour adapter les préconisations et développer des outils d'aide à la décision efficaces : le cas de la lutte contre les mauvaises herbes et de l'outil DEC ID'Herb

National audienc

Pea TILLING platform

International audienc

Genomic ressources for pea

International audienc

Exploiting post-genomics resources in model species and genome conservation between model species and legume crops to accelerate seed biology discoveries in crops

International audienc

Examples of mutant phenotypes representing the nine major phenotypic groups

Plant 566: cotyledon color, albino. Plant 939: plantlet architecture, bushy; plant architecture, hyper compact; leaf color, pale green; stem size, extreme dwarf. Plant 54: plant architecture, determinate growth. Plant 1,236: plant architecture, basal branching; leaf color, pale green, yellow; leaf size, medium; stem size, dwarf. Plant 903: leaf, cone shaped at leaf base; flowers, sterile flowers. Plant 1,567: leaf, distorted; stipule, silver-argentous. Plant 630: flowers, cauliflower type inflorescence; flowers, abnormal all; stem, dwarf; leaf, upcurling.<p><b>Copyright information:</b></p><p>Taken from "UTILLdb, a forward and reverse genetics tool"</p><p>http://genomebiology.com/2008/9/2/R43</p><p>Genome Biology 2008;9(2):R43-R43.</p><p>Published online 26 Feb 2008</p><p>PMCID:PMC2374714.</p><p></p