3,338 research outputs found
Effects of ursodeoxycholic acid on synthesis of cholesterol and bile acids in healthy subjects
Background/Aims: Ursodeoxycholic acid ( UDCA) decreases biliary secretion of cholesterol and is therefore used for the dissolution of cholesterol gallstones. It remains unclear whether these changes in biliary cholesterol excretion are associated with changes in cholesterol synthesis and bile acid synthesis. We therefore studied the activities of rate-limiting enzymes of cholesterol synthesis and bile acid synthesis, 3-hydroxy-3-methyl-glutarylcoenzyme A reductase and cholesterol 7alpha-hydroxylase, respectively, in normal subjects during UDCA feeding. Methods: UDCA was given to 8 healthy volunteers ( 5 men, 3 women; age 24-44 years) in a single dose of 10-15 mg/kg body weight for 40 days. Before and during ( days 3, 5, 10, 20, 30 and 40) UDCA treatment, urinary excretion of mevalonic acid and serum concentrations of 7alpha-hydroxy-4-cholesten-3-one (7alpha-HCO) were determined as markers of cholesterol and bile acid synthesis, respectively. The Wilcoxon signed rank test and Spearman's rank correlation coefficient were used for statistical analysis. Results: Cholesterol synthesis and serum lipid concentrations remained unchanged during UDCA treatment for 40 days. However, synthesis of bile acids increased during long-term treatment with UDCA as reflected by an increase in 7alpha-HCO serum concentrations from 39.7 +/- 21.3 ng/ml (median 32.8 ng/ml) before treatment to 64.0 +/- 30.4 ng/ml (median 77.5 ng/ml) at days 30-40 of UDCA treatment ( p < 0.05). Conclusions: UDCA treatment does not affect cholesterol synthesis in the liver, but does increase bile acid synthesis after prolonged treatment. This may represent a compensatory change following decreased absorption of endogenous bile acids as observed with UDCA therapy
Raman cooling and heating of two trapped Ba+ ions
We study cooling of the collective vibrational motion of two 138Ba+ ions
confined in an electrodynamic trap and irradiated with laser light close to the
resonances S_1/2-P_1/2 (493 nm) and P_1/2-D_3/2 (650 nm). The motional state of
the ions is monitored by a spatially resolving photo multiplier. Depending on
detuning and intensity of the cooling lasers, macroscopically different
motional states corresponding to different ion temperatures are observed. We
also derive the ions' temperature from detailed analytical calculations of
laser cooling taking into account the Zeeman structure of the energy levels
involved. The observed motional states perfectly match the calculated
temperatures. Significant heating is observed in the vicinity of the dark
resonances of the Zeeman-split S_1/2-D_3/2 Raman transitions. Here two-photon
processes dominate the interaction between lasers and ions. Parameter regimes
of laser light are identified that imply most efficient laser cooling.Comment: 8 pages, 5 figure
Quantum jumps in hydrogen-like systems
In this paper it is shown that the Lyman- transition of a single
hydrogen-like system driven by a laser exhibits macroscopic dark periods,
provided there exists an additional constant electric field. We describe the
photon-counting process under the condition that the polarization of the laser
coincides with the direction of the constant electric field. The theoretical
results are given for the example of . We show that the emission
behavior depends sensitively on the Lamb shift (W.E. Lamb, R.C. Retherford,
Phys. Rev. 72, 241 (1947)) between the and energy levels.
A possibly realizable measurement of the mean duration of the dark periods
should give quantitative information about the above energy difference by using
the proposed photon-counting process.Comment: 7 pages RevTeX + 2 figures Phys. Rev A accepte
DNA aberrations in urinary bladder cancer detected by flow cytometry and FISH
Detection of molecular alterations is of potential significance for diagnosis and prognosis in bladder cancer. Fluorescence in situ hybridization (FISH) allows visualization and quantitation of genes and chromosomes on a cell by cell level and can easily be applied to urinary cells. To evaluate the sensitivity of FISH for detection of DNA aberrations in bladder cancer, formalin-fixed tissues of 293 tumors were examined by FISH and flow cytometry (FCM). Centromere probes for the chromosomes X, Y, 1, 7, 9, and 17 were used for FISH analysis. FISH was more sensitive for detection of quantitative DNA aberrations than FCM. An aberration of at least one chromosome was found in 107 of 108 tumors (99%), which were tetraploid, aneuploid, or multiploid, and in 29 of 49 tumors (59%), which were diploid, by FCM. The frequency of FISH aberrations showed greater differences between pTa (47%) and pT1 tumors (85%;P<0.0001) than between stages pT1 and pT2-4 (98%). The marked genetic difference between pTa and pT1 tumors argues against the concept of grouping pTa and pT1 tumors together as "superficial bladder cancer.â The frequency of tumors with chromosomal aberrations detected by FISH increased with the number of chromosomes examined. Aneusomy was seen in 68% of grade 1 tumors examined for â„4 chromosomes, suggesting that the cytological diagnosis of bladder cancer recurrences could be substantially improved by FIS
Transmission resonances and supercritical states in a one dimensional cusp potential
We solve the two-component Dirac equation in the presence of a spatially one
dimensional symmetric cusp potential. We compute the scattering and bound
states solutions and we derive the conditions for transmission resonances as
well as for supercriticality.Comment: 10 pages. Revtex 4. To appear in Phys Rev.
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