Ultrasonic inspection of flooded mineshafts for stability monitoring

This is the author accepted manuscript.The final version is available from Maney via the DOI in this record.Inspecting abandoned mine shafts is critical in ensuring their safety through early identification of signs of deterioration. Since the common inspection methods of CCTV and LiDAR are not very effective underwater, two modules have been designed for inspecting the linings of flooded, abandoned mine shafts. Using sonar technology, they allow the early stages of degradation to the lining to be detected which – since this could be indicative of imminent collapse – provides protection against the consequential risk to property and human life. Detailed measurements of several shafts’ cross-sections have been recorded using profiling and imaging sonar technology. Although imaging sonar provides very different results in the confined and reverberant environment of a mine shaft, compared to its more common environment of a seabed, it was shown that when combined with the profiling sonar, it allows shafts to be surveyed in a shorter period of time and improves the reliability of the profiling function.European Commissio

Integration of microRNA signatures of distinct mammary epithelial cell types with their gene expression and epigenetic portraits

Introduction: MicroRNAs (miRNAs) have been implicated in governing lineage specification and differentiation in multiple organs; however, little is known about their specific roles in mammopoiesis. We have determined the global miRNA expression profiles of functionally distinct epithelial subpopulations in mouse and human mammary tissue, and compared these to their cognate transcriptomes and epigenomes. Finally, the human miRNA signatures were used to interrogate the different subtypes of breast cancer, with a view to determining miRNA networks deregulated during oncogenesis. Methods: RNA from sorted mouse and human mammary cell subpopulations was subjected to miRNA expression analysis using the TaqMan MicroRNA Array. Differentially expressed (DE) miRNAs were correlated with gene expression and histone methylation profiles. Analysis of miRNA signatures of the intrinsic subtypes of breast cancer in The Cancer Genome Atlas (TCGA) database versus those of normal human epithelial subpopulations was performed. Results: Unique miRNA signatures characterized each subset (mammary stem cell (MaSC)/basal, luminal progenitor, mature luminal, stromal), with a high degree of conservation across species. Comparison of miRNA and transcriptome profiles for the epithelial subtypes revealed an inverse relationship and pinpointed key developmental genes. Interestingly, expression of the primate-specific miRNA cluster (19q13.4) was found to be restricted to the MaSC/basal subset. Comparative analysis of miRNA signatures with H3 lysine modification maps of the different epithelial subsets revealed a tight correlation between active or repressive marks for the top DE miRNAs, including derepression of miRNAs in Ezh2-deficient cellular subsets. Interrogation of TCGA-identified miRNA profiles with the miRNA signatures of different human subsets revealed specific relationships. Conclusions: The derivation of global miRNA expression profiles for the different mammary subpopulations provides a comprehensive resource for understanding the interplay between miRNA networks and target gene expression. These data have highlighted lineage-specific miRNAs and potential miRNA-mRNA networks, some of which are disrupted in neoplasia. Furthermore, our findings suggest that key developmental miRNAs are regulated by global changes in histone modification, thus linking the mammary epigenome with genome-wide changes in the expression of genes and miRNAs. Comparative miRNA signature analyses between normal breast epithelial cells and breast tumors confirmed an important linkage between luminal progenitor cells and basal-like tumors.Bhupinder Pal, Yunshun Chen, Andrew Bert, Yifang Hu, Julie M. Sheridan, Tamara Beck, Wei Shi, Keith Satterley, Paul Jamieson, Gregory J. Goodall, Geoffrey J. Lindeman, Gordon K. Smyth, and Jane E. Visvade

A Transcription and Translation of Sloane MS. 2131, Robert Ashley’s (1561-1641) Vita: with Additional Biographical Details

British Library Sloane MS. 2131, Vita, is an autobiography written in Latin by Robert Ashley (1565-1641), bibliophile, lawyer, and translator. Ashley bequeathed his collection of approximately 5000 books to establish a library at Middle Temple, one of the four Inns of Court. This is the first full transcription and translation of Ashley’s manuscript to be published. Ashley’s Vita has been cited in a variety of publications, ranging from studies on dreams and children’s literature, to his role as a political agent in the late sixteenth century, but there has been no systematic examination of his autobiography in the context of early modern English biographical studies

A Transcription and Translation of Sloane MS. 2131, Robert Ashley’s (1561-1641) Vita: with Additional Biographical Details

British Library Sloane MS. 2131, Vita, is an autobiography written in Latin by Robert Ashley (1565-1641), bibliophile, lawyer, and translator. Ashley bequeathed his collection of approximately 5000 books to establish a library at Middle Temple, one of the four Inns of Court. This is the first full transcription and translation of Ashley’s manuscript to be published. Ashley’s Vita has been cited in a variety of publications, ranging from studies on dreams and children’s literature, to his role as a political agent in the late sixteenth century, but there has been no systematic examination of his autobiography in the context of early modern English biographical studies

Vol. 22 no. 7 2006, pages 897–899 BIOINFORMATICS APPLICATIONS NOTE doi:10.1093/bioinformatics/btl025

single channel microarray dat