Effect of mutation on seed coat colour in groundnut (Arachis hypogaea L.)

The seed material of groundnut (Arachis hypogaea L.) variety namely TAG-24, is used in the present study. The mutagenized population showed significantly higher variability in the M2 and M3 generation. Mutation affecting seed coat colour were detected in M3 generation. Highest mutation frequency was induced by15 kR of gamma rays. The seed coat colour mutant showed diverse shades of colour

A new method of measuring shoulder hand behind back movement: Reliability, values in symptomatic and asymptomatic people, effect of hand dominance, and side-to-side variability

Shoulder hand behind back (HBB) range of motion (ROM) is a useful measure of impairment and treatment outcome. The purpose of this repeated measures study was to identify inter- and intra-rater reliability, of a new simplified method of measuring HBB ROM. Two experienced raters measured HBB ROM with a bubble inclinometer on 25 people (aged 42–75 years, 14 female) with unilateral shoulder dysfunction and 25 age- and gender-matched asymptomatic subjects on two different occasions. Statistical analysis included calculation of intra-class correlation coefficients (ICCs), minimal detectable change (MDC), standard error of measurement (SEM), Pearson correlation coefficient (r), coefficient of determination (R2), and the lower bound score. Mean HBB ROM was 108.6° (SD = 16.3) and 23.9° (SD = 10.5) on the pain-free and symptomatic side, respectively. Both intra-rater and inter-rater reliability were high (ICC > 0.80). For asymptomatic people the SEM was at most 3° and MDC was 8° with a strong correlation between the dominant and nondominant sides (r > 0.72). The mean absolute values and lower bound scores were at most 10.2° and 26.0°, respectively. These results indicate that this new and novel method of measuring HBB ROM is accurate, has good inter- and intra-rater reliability, and provides normal values for between-limb ROM variability

Cloud Based Security

As we all know that internet is progressing in the terms of social networking website and the site which highly interact with the user also the use spent more time on this kind of website. Also user share their personal information, some important data, photos among various people that may belong their group or community so the security for all this things is mandatory but most of the time the site doesnt give proper attention toward this security requirement by which the user information may leak by unauthorized person. so in this paper we are trying to secure the informa tion of users using different compression and encryption algorithm and the address of information from users. This data is stored at various places on internet which is scattered on worldwide . So when any authorized person is trying to access his data that person only get all the information about his data but still he will never know the location of that data. The system we developed in thi s paper allows the user to upload all his data in any format including the security feature like compression and encry ption. This all uploaded data and information can be access from anywhere in the world. So finally we can say that we developed the very secure system to store the important info rmation on website in a very effective & secured manner

Studies on Characteristic of Extracellular L–Glutaminase and Identification of L-Glutaminase Producing Bacterial Strain from Cattle Feeding Farm of Chhatrapati Sambhajinagar, Maharashtra State, India

L-glutaminase is gaining marked importance due to its potential clinical applications. A variety of microorganisms, including bacteria, yeast, and lamentous fungi, from soil habitat have been reported to produce L-glutaminase. The present investigation was carried out to isolate and screening of L-glutamiase producing bacteria from soil samples of cattle feeding sites. Among twenty three soil samples of cattle feeding farms around Aurangabad, nineteen bacteria’s were isolated. From that one isolate (AGT-19) that showed significant L-glutaminase activity. Morphological characteristics and 16S rRNA sequencing were used for phylogenetic analysis to identified strain AGT-19 as Kurthia gibsonii. Moreover, Kurthia gibsonii was grown in a medium containing 2.5% succinate and 2.5% L-glutamine showed significant activity of L-glutaminase (0.079 U/mg). The optimum conditions for L-glutaminase production were temperature was 39°C and pH was 7.0. The present isolation of K. gibsonii from cattle feeding site indicated that this bacterium is suitably adapted to the environment of excretion and to point of forming a microbiota in the fecal product. It revealed that soil of cattle feeding farm has diverse bacterial strains and its habitat nature allowed the bacteria expressed the protease activity

Inhibition of pathogenic bacterial biofilms on PDMS based implants by L. acidophilus derived biosurfactant

Abstract Background Lactobacillus spp. predominantly shows its presence as a normal mucosal flora of the mouth and intestine. Therefore, the objective of our research is to investigate the in-vitro conditions for the prospective of medically valuable biosurfactants (BSs) derived from Lactobacillus spp. Biosurfactant (BS) obtained from Lactobacillus spp. exhibit antibiofilm and antiadhesive activity against broad range of microbes. In the present study we investigated the production, purification and properties of key components of the cell-associated-biosurfactant (CABS) from Lactobacillus acidophilus NCIM 2903. Results Extracted, purified, freeze-dried CABS shows reduction in surface tension (SFT) of phosphate buffer saline (PBS @pH 7.0) from 71 to 26 mN/m and had a critical micelle concentration (CMC) of 23.6 mg/mL. The CABS showed reduction in interfacial tension (IFT) against various hydrocarbons and had effective spreading capability as reflected through the decrease in contact angle (CA) on different surfaces (polydimethylsiloxane - PDMS, Teflon tape, glass surface, polystyrene film and OHP sheet). The anionic nature of CABS displayed stability at different pH and temperatures and formed stable emulsions. Thin layer chromatography (TLC) and Fourier transform infrared spectroscopy (FTIR) revealed CABS as glycolipoprotein type. The Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE) showed presence of multiple bands in a molecular range of 14.4 to 60 kDa, with prominent bands of 45 kDa. The CABS has significant antiadhesion and antibiofilm activity against tested bacterial strains. Conclusion The current challenging situation is to develop methods or search for the molecules that will prevent the formations of biofilm on medical bioimplants of PDMS based materials. These findings are supportive for the use of Lactobacilli derived BS as potential antiadhesive agent on various surfaces of biomedical devices

In Vitro Cytotoxicity Efficiency of Extracellular L-Glutaminase Produce by Mesophilic Bacteria, Kurthia Gibsonii on Lncap, MDA-MB 231 and Hepg-2 Cell Lines

Mesophilic bacteria from soil habitat have been reported to produce extracellular L-glutaminase. The present study was carried out to anticancer screening of L-glutamiase producing bacteria (Kurthia gibsonii) from soil sample of cattle feeding site from Satara parisar, Chhatrapati Sambhajinagar. Results showed, among three soil samples of cattle feeding farms, Kurthia gibsonii was isolated. From that exhibited the highest L-glutaminase activity. Moreover, the in vitro cytotoxic activity of L-glutaminase against the (Lymph Node Carcinoma of the Prostate) LNCaP, (an epithelial, human breast cancer cell line) MDA-MB 231 and hepatocellular (HepG-2) carcinoma cell lines at different concentration (0.47, 0.94, 1.88, 3.75, 7.50, 15.00, 30.00 and 60.00 μg/ml) by the MTT assay and compared with the standard Doxrubcin. The antitumor effect against human liver carcinoma cell line revealed that L-glutaminase produced by Kurthia gibsonii showed potent cytotoxic activity of tested cell line in a dose-dependent manner with an LC50 value of 4.1 μg/ml

Lactobacillus acidophilus Derived Biosurfactant as a Biofilm Inhibitor: A Promising Investigation Using Microfluidic Approach

Background: Biomedical devices and implants are adversely affected by biofilm-associated infections that pose serious public health issues. Biosurfactants (BSs) can combat pathogenic biofilms through their antimicrobial, antibiofilm and antiadhesive capabilities. The objective of our research was to produce biosurfactant (BS) from Lactobacillus acidophilus NCIM 2903 and investigate its antibiofilm, antiadhesive potential using microfluidics strategies by mimicking the micro-environment of biofilm. Methods: Antibiofilm and antiadhesive potential was effectively evaluated using different methods like microfluidics assay, catheter assay, polydimethlysiloxane (PDMS) disc assay. Along with this chemical and physical characteristics of BS were also evaluated. Results: Cell free biosurfactant (CFBS) obtained was found to be effective against biofilm which was validated through the microfluidic (MF) or Lab on Chip (LOC) approach. The potency of CFBS was also evaluated on catheter tubing and PDMS surfaces (representative bioimplants). The efficacy of CFBS was also demonstrated through the reduction in surface tension, interfacial tension, contact angle and low critical micelle concentration. Conclusion: CFBS was found to be a potent antimicrobial and antibiofilm agent. We believe that perhaps this is the first report on demonstrating the inhibiting effect of Lactobacillus spp. derived CFBS against selected bacteria via LOC approach. These findings can be explored to design various BSs based formulations exhibiting antimicrobial, antibiofilm and antiadhesive potential for biomedical applications

The many hats of transmembrane emp24 domain protein TMED9 in secretory pathway homeostasis

The secretory pathway is an intracellular highway for the vesicular transport of newly synthesized proteins that spans the endoplasmic reticulum (ER), Golgi, lysosomes and the cell surface. A variety of cargo receptors, chaperones, and quality control proteins maintain the smooth flow of cargo along this route. Among these is vesicular transport protein TMED9, which belongs to the p24/transmembrane emp24 domain (TMED) family of proteins, and is expressed across vertebrate species. The TMED family is comprised of structurally-related type I transmembrane proteins with a luminal N-terminal Golgi-dynamics domain, a luminal coiled-coil domain, a transmembrane domain and a short cytosolic C-terminal tail that binds COPI and COPII coat proteins. TMED9, like other members of the TMED family, was first identified as an abundant constituent of the COPI and COPII coated vesicles that mediate traffic between the ER and the Golgi. TMED9 is typically purified in hetero-oligomers together with TMED family members, suggesting that it may function as part of a complex. Recently, TMED family members have been discovered to play various roles in secretory pathway homeostasis including secreted protein processing, quality control and degradation of misfolded proteins, and post-Golgi trafficking. In particular, TMED9 has been implicated in autophagy, lysosomal sorting, viral replication and cancer, which we will discuss in this Mini-Review

Synthesis and Characterisation of Bis-azido Methyl Oxetane and its Polymer and Copolymer with Tetrahydrofuran

Bis-azido methyl oxetane (BAMO) was synthesised from pentaerythritol in two steps. Pentaerythritol was chlorinated to yield a mixture of mono, di, tri and tetra chloro compounds. The trichloro compound on ring closure gives bis-chloro methyl oxetane (BCMO). It was reacted with sodium azide in aqueous medium to obtain BAMO. The latter was polymerised using BF3 etherate catalyst and 1,4-butanediol initiator. Similarly, the BAMO- THF copolymer was also synthesised. All the monomers and polymers were characterised by IR, 1H-NMR, 13C-NMR, and refractive index. The polymers were also characterised for molecular weight, hydroxyl value, etc. Thermal analysis showed that both polymers degrade exothermically with T max of 237 °C for poly BAMO and 241°C for BAMO- THF copolymer with activation energy of 39 kcal/mol and 40 kcal/mol, respectively. Explosive properties like impact and friction sensitivity of BAMO and the other polymers were also determined