Air pollution impacts on forests in a changing climate

Growing awareness of air pollution effects on forests has, from the early 1980s on, led to intensive forest damage research and monitoring. This has fostered air pollution control, especially in Europe and North America, and to a smaller extent also in other parts of the world. At several forest sites in these regions, there are first indications of a recovery of forest soil and tree conditions that may be attributed to improved air quality. This caused a decrease in the attention paid by politicians and the public to air pollution effects on forests. But air pollution continues to affect the structure and functioning of forest ecosystems not only in Europe and North America but even more so in parts of Russia, Asia, Latin America, and Africa. At the political level, however, attention to climate change is focussed on questions of CO2 emission and carbon sequestration. But ecological interactions between air pollution including CO2 and O3 concentrations, extreme temperatures, drought, fire, insects, pathogens, and fire, as well as the impact of ecosystem management practices, are still poorly understood. Future research should focus on the interacting impacts on forest trees and ecosystems. The integrative effects of air pollution and climatic change, in particular elevated O3, altered nutrient, temperature, water availability, and elevated CO2, will be key issues for impact research. An important improvement in our understanding might be obtained by the combination of long-term multidisciplinary experiments with ecosystem-level monitoring, and the integration of the results with ecosystem modelling within a multiple-constraint framework

Epicuticular wax concentration on Syzygium myrtifolium leaves

The pollution issues are a pervasive and increasing threat to human and forest ecosystem. Plants have been recognised as a method to reduce pollution. However, leaf is the most sensitive organ to pollution where changes in leaf morphological, physiological, anatomical and biochemical can occur. This study was conducted to determine the quantity of epicuticular wax on Syzygium myrtifolium leaves in urban, suburban and industrial areas. Three trees were selected in each of the study sites and new leaves on the branch was marked. 5 g of samples were collected for analysis. Leaf surface particles were extracted by using 20 ml of chloroform in conical flask and was left overnight for hardening where weight were recorded. Data analysis showed that the mean epicuticular wax ranged from 267.2-680.7 µg/m2 with a mean increment of 309.6-950.7 µg/m2 /yr. The epicuticular wax concentration on S. myrtifolium leaves was significantly higher in industrial areas compared to those in the urban and suburban areas. S. myrtifolium at industrial areas are in the open and subjected to direct sunlight and also responding to stress caused by the air pollutions. The low concentration of epicuticular wax was recorded on S. myrtifolium leaves in urban area. These trees are subjected to high concentration of pollutants especially from exhaust emissions and possible is due to the aerosol deposition which have been carried by the wind. This initial study reveal that the level of physiological stress on the trees in industrial, suburban and urban areas are at a different scale where the levels of air pollution is different. Understanding the tree traits are important to capitalize their role as bio filters. This study provide the baseline data for future study where the relationship between the particulate matters deposited on the tree canopy and the tree trait could be explained

Strategic roadmap to assess forest vulnerability under air pollution and climate change

Although it is an integral part of global change, most of the research addressing the effects of climate change on forests have overlooked the role of environmental pollution. Similarly, most studies investigating the effects of air pollutants on forests have generally neglected the impacts of climate change. We review the current knowledge on combined air pollution and climate change effects on global forest ecosystems and identify several key research priorities as a roadmap for the future. Specifically, we recommend (1) the establishment of much denser array of monitoring sites, particularly in the South Hemisphere; (2) further integration of ground and satellite monitoring; (3) generation of flux-based standards and critical levels taking into account the sensitivity of dominant forest tree species; (4) long-term monitoring of N, S, P cycles and base cations deposition together at global scale; (5) intensification of experimental studies, addressing the combined effects of different abiotic factors on forests by assuring a better representation of taxonomic and functional diversity across the similar to 73,000 tree species on Earth; (6) more experimental focus on phenomics and genomics; (7) improved knowledge on key processes regulating the dynamics of radionuclides in forest systems; and (8) development of models integrating air pollution and climate change data from long-term monitoring programs.</p

Biocompatible fluorescent silicon nanocrystals for single-molecule tracking and fluorescence imaging.

Fluorescence microscopy is used extensively in cell-biological and biomedical research, but it is often plagued by three major problems with the presently available fluorescent probes: photobleaching, blinking, and large size. We have addressed these problems, with special attention to single-molecule imaging, by developing biocompatible, red-emitting silicon nanocrystals (SiNCs) with a 4.1-nm hydrodynamic diameter. Methods for producing SiNCs by simple chemical etching, for hydrophilically coating them, and for conjugating them to biomolecules precisely at a 1:1 ratio have been developed. Single SiNCs neither blinked nor photobleached during a 300-min overall period observed at video rate. Single receptor molecules in the plasma membrane of living cells (using transferrin receptor) were imaged for ≥10 times longer than with other probes, making it possible for the first time to observe the internalization process of receptor molecules at the single-molecule level. Spatial variations of molecular diffusivity in the scale of 1-2 µm, i.e., a higher level of domain mosaicism in the plasma membrane, were revealed

Effectiveness of Bystander-Initiated Cardiac-Only Resuscitation for Patients With Out-of-Hospital Cardiac Arrest

[Background] Previous animal and clinical studies suggest that bystander-initiated cardiac-only resuscitation may be superior to conventional cardiopulmonary resuscitation (CPR) for out-of-hospital cardiac arrests. Our hypothesis was that both cardiac-only bystander resuscitation and conventional bystander CPR would improve outcomes from out-of-hospital cardiac arrests of ≤15 minutes' duration, whereas the addition of rescue breathing would improve outcomes for cardiac arrests lasting >15 minutes. [Methods and Results] We carried out a prospective, population-based, observational study involving consecutive patients with emergency responder resuscitation attempts from May 1, 1998, through April 30, 2003. The primary outcome measure was 1-year survival with favorable neurological outcome. Multivariable logistic regression analysis was performed to evaluate the relationship between type of CPR and outcomes. Among the 4902 witnessed cardiac arrests, 783 received conventional CPR, and 544 received cardiac-only resuscitation. Excluding very-long-duration cardiac arrests (>15 minutes), the cardiac-only resuscitation yielded a higher rate of 1-year survival with favorable neurological outcome than no bystander CPR (4.3% versus 2.5%; odds ratio, 1.72; 95% CI, 1.01 to 2.95), and conventional CPR showed similar effectiveness (4.1%; odds ratio, 1.57; 95% CI, 0.95 to 2.60). For the very-long-duration arrests, neurologically favorable 1-year survival was greater in the conventional CPR group, but there were few survivors regardless of the type of bystander CPR (0.3% [2 of 624], 0% [0 of 92], and 2.2% [3 of 139] in the no bystander CPR, cardiac-only CPR, and conventional CPR groups, respectively; P<0.05). [Conclusions] Bystander-initiated cardiac-only resuscitation and conventional CPR are similarly effective for most adult out-of-hospital cardiac arrests. For very prolonged cardiac arrests, the addition of rescue breathing may be of some help