Ethics for civil indoor drones: a qualitative analysis

[EN] Drones face two main concerns: safety and security/privacy. Whilst safety has been broadly studied by literature, less research has been carried out into security/privacy. Moreover, current European regulations on drone flights apply to outdoor drones but not always to their indoor counterparts. However, several industrial sectors have started to use drones for indoor tasks such as surveillance, architecture, emergencies, and communication media. A qualitative study has been conducted in order to explore the concerns expressed by civil drone operators over the measures that manufacturers include in their products and information packages. Codes of conduct could also help these parties when there is no legal regulation that can be applied. We used content analysis as the method of analysis for three different sources: secondary data from a literature review and from public European documents, and primary data from focus groups. Results show that safety and security/privacy by design are seen as the best ethical measures, whilst codes of conduct could be used as complimentary information for professional users.The author(s) disclosed receipt of the following financial support for the research, authorship and/or publication of this article: The project leading to this application has received funding from the European Union's Horizon 2020 research and innovation programme under grant agreement No. 732433. Project: AiRT, Technology transfer of RPAs for the creative industry, H2020-ICT-2016-2017.De-Miguel-Molina, M.; Santamarina-Campos, V.; Carabal-Montagud, M.; De-Miguel-Molina, B. (2018). Ethics for civil indoor drones: a qualitative analysis. International Journal of Micro Air Vehicles. 10(4):340-351. https://doi.org/10.1177/1756829318794004S34035110

Metabolic engineering to simultaneously activate anthocyanin and proanthocyanidin biosynthetic pathways in Nicotiana spp

[EN] Proanthocyanidins (PAs), or condensed tannins, are powerful antioxidants that remove harmful free oxygen radicals from cells. To engineer the anthocyanin and proanthocyanidin biosynthetic pathways to de novo produce PAs in two Nicotiana species, we incorporated four transgenes to the plant chassis. We opted to perform a simultaneous transformation of the genes linked in a multigenic construct rather than classical breeding or retransformation approaches. We generated a GoldenBraid 2.0 multigenic construct containing two Antirrhinum majus transcription factors (AmRosea1 and AmDelila) to upregulate the anthocyanin pathway in combination with two Medicago truncatula genes (MtLAR and MtANR) to produce the enzymes that will derivate the biosynthetic pathway to PAs production. Transient and stable transformation of Nicotiana benthamiana and Nicotiana tabacum with the multigenic construct were respectively performed. Transient expression experiments in N. benthamiana showed the activation of the anthocyanin pathway producing a purple color in the agroinfiltrated leaves and also the effective production of 208.5 nmol (-) catechin/g FW and 228.5 nmol (-) epicatechin/g FW measured by the p-dimethylaminocinnamaldehyde (DMACA) method. The integration capacity of the four transgenes, their respective expression levels and their heritability in the second generation were analyzed in stably transformed N. tabacum plants. DMACA and phoroglucinolysis/HPLC-MS analyses corroborated the activation of both pathways and the effective production of PAs in T0 and T1 transgenic tobacco plants up to a maximum of 3.48 mg/g DW. The possible biotechnological applications of the GB2.0 multigenic approach in forage legumes to produce "bloatsafe" plants and to improve the efficiency of conversion of plant protein into animal protein (ruminal protein bypass) are discussed.This work was supported by grants BIO2012-39849-C02-01 and BIO2016-75485-R from the Spanish Ministry of Economy and Competitiveness (MINECO) (http://www.idi.mineco.gob.es/portal/site/MICINN) to LAC and a fellowship of the JAE-CSIC program to SF. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Fresquet-Corrales, S.; Roque Mesa, EM.; Sarrión-Perdigones, A.; Rochina, M.; López-Gresa, MP.; Díaz-Mula, HM.; Belles Albert, JM.... (2017). Metabolic engineering to simultaneously activate anthocyanin and proanthocyanidin biosynthetic pathways in Nicotiana spp. PLoS ONE. 12(9). https://doi.org/10.1371/journal.pone.0184839Se018483912