The qualitative method for a humanisation of research

Huge progress in scientific research, added to vast investments, have allowed to develop vaccines against the virus responsible for Sars-Cov-2 in times unimaginable, until recently (1). The use of these vaccines has documented their high efficacy and very low risk of adverse events. Undergoing vaccination campaigns around the world are changing the history of the COVID-19 pandemic. Never before has scientific research been a topic of widespread interest as in this period. [...]

Nephroblastoma with mucus-producing and argentaffin cells in a pig

Nephroblastoma is the most common primary renal neoplasm of swine and man. This report describes a swine nephroblastoma localized at the caudal pole of the left kidney, extending to the renal hilum which, on light microscopy, showed unusual mucus-producing epithelial cells of many tubules. The presence of intracytoplasmic mucin was confirmed by PAS-diastase positivity and alcianophilia both at pH 2.5 and at 1.0. Mucin-producing cells revealed cytokeratin 19 and pankeratin AE1/AE3 positivity, and some of them a chromogranin A immunohistochemical positivity; they were also positive for Grimelius stain. In veterinary medicine, even if the occurrence of mucus-producing cells in nephroblastoma has rarely been observed, this is the first description of a case of swine nephroblastoma with mucus-producing cells positive for neuroendocrine markers, thus a neuroendocrine origin was suspected

COVID-19 related lockdown: a trigger from the pre-melancholic phase to catatonia and depression, a case report of a 59 year-old man

Background: The pre-melancholic model described by Tellenbach may provide a common model for understanding the psychological implications of the lockdown. In this case report, we describe a rare catatonic status as a psychological implication linked to the COVID-19 pandemic, a really unique global situation. Case presentation: B is a 59 year-old man with mute psychiatric anamnesis whose mother suffered from a major depressive disorder. As the lockdown began, he started to develop concerns about his family’s economic condition. According to his wife, he could see no end to the epidemic and no future at all. Moving from this, he started to show a severe and rapidly progressive depression and to develop mood congruent delusions. In addition, he had increasing anhedonia, apathy, starvation and insomnia. This turned in the end into a catatonic-like state, along with a deep desire to die. Admitted to the psychiatry ward in a state of mutism, he was discharged after 15 days with a diagnosis of “Major depressive disorder, single severe episode with no psychotic behavior”. He was treated with Sertraline, Olanzapine and Lorazepam. Conclusions: Our aim is to draw attention to the effect of the lockdown upon a Tellenbach-like personality structure. Identifying this type of pre-morbid personality structure could help clinicians understand and treat some cases of patients with severe major depressive disorders elicited by the COVID-19 pandemic

Penerapan Model Pembelajaran Berbasis Masalah Untuk Meningkatkan Hasil Belajar IPA Siswa Kelas IV C SD Negeri I67 Pekanbaru

The problem this research is the students achievement of social fourth graderes studies still low with an average value of 75,60 and minimum completeness criteria (KKM) social studies is 81. Between students, amounting to 40 people only 12 students who achieve classical KKM with 30%. This research is Classroom Action Research (CAR), which aims to improve the student achievement of science studies class IVc at SDN 167 Pekanbaru with implementation model problem instruction. Formulation of the problem : Is the implementation of problem instruction can improve students achievement of social studies at class IVc SDN 167 Pekanbaru. The research was conducted month April. Subjects were students of SDN 167 Pekanbaru, totaling 40 people who use the data source. The data collection instruments in this thesis is a teacher and students activities sheets and students achievement. This thesis presents the results obtained each day before the action an improve in base score cycle with the average being 75,60. In the first cycle improve an average of 77,50 with increase big as 84,12. Activities of the teacher in the learning process in cycle of 1with increase big as and the second meeting improve to 71,44% and the second meeting improve 82,14%. Cycle II first meeting and the second meeting improve 85,71% to 92,85%. In the third cycle of the first meeting and the second meeting improve 73,07% to 76,92%. Results of data analysis of students activities in the first cycle with the first meeting of an average of 85,76% and a second meeting improve to 90,38% in the class IVc at SDN 167 Pekanbaru that the implementation of problem inkuiri can improve the student achievement of science studiesat fourth graderes SDN 167 Pekanbaru

Airway Remodeling in Feline Lungs

Airway remodeling encompass structural changes that occur as the result of chronic injury and lead to persistently altered airway structure and function. Although this process is known in several human respiratory conditions such as asthma and chronic obstructive pulmonary disease (COPD), airway remodeling is poorly characterized in the feline counterpart. In this study, we describe the spontaneous pulmonary changes in 3 cats paralleling the airway remodeling reported in humans. We observed airway smooth muscle cells (ASMCs) hyperplasia (peribronchial and interstitial), airway subepithelial and interstitial fibrosis, and vascular remodeling by increased number of vessels in the bronchial submucosa. The hyperplastic ASMCs co-expressed α-SMA, vimentin and desmin suggesting that vimentin, which is not normally expressed by ASMCs, may play a role in airway thickening, and remodeling. ASMCs had strong cytoplasmic expression of TGFβ-1, which is known to contribute to tissue remodeling in asthma and in various bronchial and interstitial lung diseases, suggesting its involvement in the pathogenesis of ASMCs hyperplasia. Our findings provide histologic evidence of airway remodeling in cats. Further studies on larger caseloads are needed to support our conclusions on the value of this feline condition as an animal model for nonspecific airway remodeling in humans

The role of new technologies to prevent suicide in adolescence. a systematic review of the literature

Background and objectives: Suicide in adolescents represents a major public health concern. To date, a growing number of suicide preventive strategies based on the use of new technologies are emerging. We aimed to provide an overview of the present literature on the use of new technologies in adolescent suicide prevention. Materials and methods: An electronic search was run using the following keywords: Technology OR Technologies OR APP OR Application OR mobile application) AND (Adolescent OR youth OR puberty) AND (Suicid* OR Self-harm OR self-destruction). Inclusion criteria were: English language, published in a peer-reviewed journal, suicide prevention with the use of new technologies among adolescents. Results: Our search strategy yielded a total of 12 studies on the use of telemedicine, 7 on mobile applications, and 3 on language detection. We also found heterogeneity regarding the study design: 3 are randomized controlled trials (RCT), 13 are open-label single group trials, 2 are randomized studies, and 1 is a cross-sectional study. Telemedicine was the most adopted tool, especially web-based approaches. Mobile applications mostly focused on screening of depressive symptoms and suicidal ideation, and for clinical monitoring through the use of text messages. Although telepsychiatry and mobile applications can provide a fast and safe tool, supporting and preceding a face-to-face clinical assessment, only a few studies demonstrated efficacy in preventing suicide among adolescents through the use of these interventions. Some studies suggested algorithms able to recognize people at risk of suicide from the exploration of the language on social media posts. Conclusions: New technologies were found to be well accepted and tolerated supports for suicide prevention in adolescents. However, to date, few data support the use of such interventions in clinical practice and preventive strategies. Further studies are needed to test their efficacy in suicide prevention among adolescents and young adults

A double histochemical/immunohistochemical staining for the identification of canine mast cells in light microscopy

Immunohistochemistry (IHC) is a widely used technique in diagnostic pathology, but the simultaneous analysis of more than one antibody at a time with different chromogens is rather complex, time‐consuming, and quite expensive. In order to facilitate the identification of mast cells (MCs) during immunohistochemical analysis of membrane and/or nuclear markers, we propose a new staining method that includes the association of IHC and toluidine blue as a counterstain. To achieve this goal, we tested c‐kit, Ki67, and cannabinoid receptor 2 on several cases of cutaneous canine mast cell tumors (MCTs), cutaneous mastocytosis, and atopic dermatitis. The results obtained show how this double staining technique, although limited to non‐cytoplasmic markers and of little use in poorly differentiated MCTs in which MC metachromasia is hard to see, can be used during the evaluation of nuclear and/or membranous immunohistochemical markers in all canine cutaneous disorders, especially if characterized by the presence of a low number of MCs. It can help to evaluate those MCTs in which neoplastic MCs must be clearly distinguished from inflammatory cells that can infiltrate the tumor itself, in facilitating the calculation of the Ki67 index. Moreover, it can be used to study the expression of new markers in both animal and human tissues containing MCs and in MC disorders

The safety of care focused on patient identity: an observational study

BACKGROUND AND AIM: Healthcare organizations, in order to reduce errors and extend the number of safe practices, are looking for possible solutions to enhance the clients' health quality care and trying to spread the culture of safety healthcare. Although in the literature the field of research "patient safety" is very debated, there are few empirical studies that investigate about the strategies undertaken by nursing students for the patients identification process during their care pathway. The aim of this study is to investigate the knowledge of the Ministerial Recommendation No. 3/2008 among nursing students, a specific Italian directive that aims to guarantee the safety of cares. METHODS: A four-weeks single-center observational study was conducted, involving a convenient sample of 112 students of the 2nd and 3rd  year of the Nursing Course Degree of the University of Parma. The survey was conducted using an ad-hoc questionnaire. RESULTS: The use of the identification wristband is considered one of the most important strategy to make sure the patient identification; unfortunately it is in practice used just in few occasions and only when performed specific procedures; it is furthermore noted that patients are not enough informed about the use and finalities of the identification wristband. CONCLUSIONS: Considering the importance of the patient identification process to guarantee the  safety of cares, the results produced, suggest that this investigation field deserves further insights in order to collect more substantial data and expand knowledge on the specific subject, so as to fill  knowledge gaps and sensitize nursing students to the correct use of the identification wristband

Nomophobia in healthcare: an observational study between nurses and students

BACKGROUND AND AIM: The abuse of technical devices can be considered a form of addiction that is defined in current literature as Nomophobia. The phenomenon appears to be quite widespread among nurses and nursing students, and nomophobic behaviours of professionals can lead to a reduction in the quality of the care provided. The aim of this study is to investigate the situation in a University and in a hospital in northern Italy and evaluate the levels of nomophobia among nurses and students. METHODS: An observational study was conducted with a questionnaire using a sample of nursing students and nurses.  Both of the studies had the common goal of investigating the levels of nomophobia and   evaluate the comparison between the two groups. RESULTS: In general, neither nurses nor students seem to demonstrate nomophobia's critical levels. The comparison of the daily use of the smartphone between nurses and students shows that the frequent use of students is balanced by the nurses' daily trend line that progressively decreases. Both nurses and students affirm to use their smartphones at work with a certain frequency although, especially among the students, it is usually for appropriate and justified reasons. CONCLUSIONS: It is necessary to consider the development of an educational project that regulates the use of smartphones, explaining the meaning of nomophobia, right from the first year of the University experience so as to correctly address the students'  (and future professionals') behaviours  to make them aware of the misuse of mobile phones, especially in the clinical setting that can easily cause distractions and consequently irreversible  errors

Gender and sex bias in prevention and clinical treatment of women's chronic pain: hypotheses of a curriculum development

This discursive paper focuses on undergraduate medical education's role in tackling gender bias in clinical practice, specifically preventing and managing from a non-biomedical perspective chronic pain in women. A preliminary web search of medical schools' curricula was performed to identify programs content related to gender bias in pain management. The web search included 10 universities' websites selected from the top 10 rankings QS Universities Rankings 2022 for medical schools. Additionally, a questionnaire was sent to all deans of the selected academic institutions to explore the curriculum content further. The web search, and the lack of response from the deans, highlighted that relevant curriculum components on gender bias and chronic pain needed to be implemented. Therefore, this paper introduces an innovative curriculum development approach designed by the multi-professional research team to be implemented in medical school programs. This novel educational strategy could also cross-contaminate other healthcare practitioners' university programs and, thus, stimulate an interprofessional debate into fostering inclusiveness and equal opportunities in health