Pharmacological perturbation of CXCL1 signaling alleviates neuropathogenesis in a model of HEVA71 infection

Hand, foot and mouth disease (HFMD) caused by Human Enterovirus A71 (HEVA71) infection is typically a benign infection. However, in minority of cases, children can develop severe neuropathology that culminate in fatality. Approximately 36.9% of HEVA71-related hospitalizations develop neurological complications, of which 10.5% are fatal. Yet, the mechanism by which HEVA71 induces these neurological deficits remain unclear. Here, we show that HEVA71-infected astrocytes release CXCL1 which supports viral replication in neurons by activating the CXCR2 receptor-associated ERK1/2 signaling pathway. Elevated CXCL1 levels correlates with disease severity in a HEVA71-infected mice model. In humans infected with HEVA71, high CXCL1 levels are only present in patients presenting neurological complications. CXCL1 release is specifically triggered by VP4 synthesis in HEVA71-infected astrocytes, which then acts via its receptor CXCR2 to enhance viral replication in neurons. Perturbing CXCL1 signaling or VP4 myristylation strongly attenuates viral replication. Treatment with AZD5069, a CXCL1-specific competitor, improves survival and lessens disease severity in infected animals. Collectively, these results highlight the CXCL1-CXCR2 signaling pathway as a potential target against HFMD neuropathogenesis

MXene-Embedded Porous Carbon-Based Cu₂O Nanocomposites for Non-Enzymatic Glucose Sensors

This work explores the use of MXene-embedded porous carbon-based Cu2O nanocomposite (Cu2O/M/AC) as a sensing material for the electrochemical sensing of glucose. The composite was prepared using the coprecipitation method and further analyzed for its morphological and structural characteristics. The highly porous scaffold of activated (porous) carbon facilitated the incorporation of MXene and copper oxide inside the pores and also acted as a medium for charge transfer. In the Cu2O/ M/AC composite, MXene and Cu2O influence the sensingparameters, which were confirmed using electrochemical techniques such as cyclic voltammetry, electrochemical impedance spectroscopy, and amperometric analysis. The prepared composite shows two sets of linear ranges for glucose with a limit of detection (LOD) of 1.96 μM. The linear range was found to be 0.004 to 13.3 mM and 15.3 to 28.4 mM, with sensitivity values of 430.3 and 240.5 μA mM−1 cm−2, respectively. These materials suggest that the prepared Cu2O/M/AC nanocomposite can be utilized as a sensing material for non-enzymatic glucose sensors

MECHANISMS OF NEUROVIRULENCE IN HUMAN ENTEROVIRUS A71 NEUROPATHOGENESIS

Ph.DDOCTOR OF PHILOSOPHY (SOM

Endodontic management of maxillary second molars with two palatal root: a report of three case

The complexity of the root canal system of maxillary molars presents a constant challenge, as the dentist must have a thorough knowledge of the root canal morphology successful endodontic treatment to Provide. . This report presents the endodontic management of three cases of maxillary molars with two separate second palatal roots Tags: canal complexity, maxillary second molars, two palatal roots

Endodontic management of maxillary second molars with two palatal root: a report of three case

The complexity of the root canal system of maxillary molars presents a constant challenge, as the dentist must have a thorough knowledge of the root canal morphology successful endodontic treatment to Provide. . This report presents the endodontic management of three cases of maxillary molars with two separate second palatal roots Tags: canal complexity, maxillary second molars, two palatal roots

Pharmacological perturbation of CXCL1 signaling alleviates neuropathogenesis in a model of HEVA71 infection

10.1038/s41467-022-28533-zNature communications131890

Increased expression of heme-binding protein 1 early in Alzheimer's disease is linked to neurotoxicity

10.7554/eLife.47498ELIFE

Prunin suppresses viral IRES activity and is a potential candidate for treating enterovirus A71 infection

10.1126/scitranslmed.aar5759SCIENCE TRANSLATIONAL MEDICINE11516complete

MXene-Embedded Porous Carbon-Based Cu₂O Nanocomposites for Non-Enzymatic Glucose Sensors

This work explores the use of MXene-embedded porous carbon-based Cu2O nanocomposite (Cu2O/M/AC) as a sensing material for the electrochemical sensing of glucose. The composite was prepared using the coprecipitation method and further analyzed for its morphological and structural characteristics. The highly porous scaffold of activated (porous) carbon facilitated the incorporation of MXene and copper oxide inside the pores and also acted as a medium for charge transfer. In the Cu2O/M/AC composite, MXene and Cu2O influence the sensing parameters, which were confirmed using electrochemical techniques such as cyclic voltammetry, electrochemical impedance spectroscopy, and amperometric analysis. The prepared composite shows two sets of linear ranges for glucose with a limit of detection (LOD) of 1.96 μM. The linear range was found to be 0.004 to 13.3 mM and 15.3 to 28.4 mM, with sensitivity values of 430.3 and 240.5 μA mM–1 cm–2, respectively. These materials suggest that the prepared Cu2O/M/AC nanocomposite can be utilized as a sensing material for non-enzymatic glucose sensors