SYNTHESIS AND EVALUATION OF ANTI-INFLAMMATORY AND ANTIBACTERIAL ACTIVITIES OF SOME 1, 2-BENZISOXAZOLE DERIVATIVES

Objective: A series of 1, 2-Benzisoxazole derivatives were synthesized and characterized by various analytical techniques like Melting point, Rf, FTIR, and NMR spectra.Methods: Structures of the compounds were elucidated and evaluated for anti-inflammatory activity by HRBC membrane stabilization method, antibacterial activity against Escherichia coli and Staphylococcus aureus by cup plate method.Results: The compounds 4a and 4e showed good anti-inflammatory activity compared with standard drug Diclofenac sodium and compounds 4b and 4d showed good antibacterial activity on compared with standard drug Gentamycin.Conclusion: These compounds may serve as future leads for anti-inflammatory and antibacterial drug discovery

Large Scale Proactive Power-Quality Monitoring: An Example from Australia

In Australia and many other countries, distribution network service providers (DNSPs) have an obligation to their customers to provide electrical power that is reliable and of high quality. Failure to do so may have significant implications ranging from financial penalties theoretically through to the loss of a license to distribute electricity. In order to ensure the reliability and quality of supply are met, DNSPs engage in monitoring and reporting practice. This paper provides an overview of a large long-running power-quality monitoring project that has involved most of Australia\u27s DNSPs at one time or another. This paper describes the challenges associated with conducting the project as well as some of the important outcomes and lessons learned. A number of novel reporting techniques that have been developed as part of the monitoring project are also presented. A discussion about large-volume data management, and issues related to reporting requirements in future distribution networks is included

COMBINATORIAL EFFECT OF D-AMINOACIDS AND TETRACYCLINE AGAINST PSEUDOMONAS AERUGINOSA BIOFILM

Objective: The present study attempted to evaluate the anti-biofilm activity of D-amino acids (D-AAs) on Pseudomonas aeruginosa and determine if the combination of D-AAs with tetracycline enhances the anti-biofilm activity in vitro and ex vivo.Methods: Different D-AAs were tested for antibiofilm activity against wild type P. aeruginosa PAO1 and two multidrug resistant P. aeruginosa clinical strains in the presence of sub inhibitory concentrations of tetracycline using crystal violet microtitre plate assay. Results were further validated using in vitro wound dressing and ex vivo porcine skin models followed by cytotoxicity and hemocompatibility studies.Results: D-tryptophan (5 mmol) showed 61 % reduction in biofilm formation of P. aeruginosa. Interestingly combinatorial effect of 5 mmol D-tryptophan and 0.5 minimum inhibitory concentration (MIC) (7.5µg/ml) tetracycline showed 90% reduction in biofilm formation. 5 mmol D-methionine shows 28 % reduction and combination with tetracycline shows 41% reduction in biofilm formation of P. aeruginosa. D-leucine and D-tyrosine alone or in combination with tetracycline did not show significant anti-biofilm activity. D tryptophan-tetracycline combination could reduce 80 % and 77 % reduction in biofilm formation in two multi drug resistant P. aeruginosa clinical strains. D-tryptophan-tetracycline-combination could also reduce 76% and 66% reduction in biofilm formation in wound dressing model and porcine skin explant respectively. The cytotoxicity and hemocompatibility studies did not show significant toxicity when this combination was used.Conclusion: The results established the potential therapeutic application of D-tryptophan alone or in combination with tetracycline for treating biofilm associated clinical problems caused by P. aeruginosa

Synthesis of methylphosphonic acid by marine microbes: a source for methane in the aerobic ocean

Relative to the atmosphere, much of the aerobic ocean is supersaturated with methane; however, the source of this important greenhouse gas remains enigmatic. Catabolism of methylphosphonic acid by phosphorus-starved marine microbes, with concomitant release of methane, has been suggested to explain this phenomenon, yet methylphosphonate is not a known natural product, nor has it been detected in natural systems. Further, its synthesis from known natural products would require unknown biochemistry. Here we show that the marine archaeon Nitrosopumilus maritimus encodes a pathway for methylphosphonate biosynthesis and that it produces cell-associated methylphosphonate esters. The abundance of a key gene in this pathway in metagenomic data sets suggests that methylphosphonate biosynthesis is relatively common in marine microbes, providing a plausible explanation for the methane paradox

Temporal Modulation of Traveling Waves in the Flow Between Rotating Cylinders With Broken Azimuthal Symmetry

The effect of temporal modulation on traveling waves in the flows in two distinct systems of rotating cylinders, both with broken azimuthal symmetry, has been investigated. It is shown that by modulating the control parameter at twice the critical frequency one can excite phase-locked standing waves and standing-wave-like states which are not allowed when the system is rotationally symmetric. We also show how previous theoretical results can be extended to handle patterns such as these, that are periodic in two spatial direction.Comment: 17 pages in LaTeX, 22 figures available as postscript files from http://www.esam.nwu.edu/riecke/lit/lit.htm

Impact of a charged neighboring particle on Förster resonance energy transfer (FRET)

Förster resonance energy transfer (FRET) is an important physical phenomenon which demands precise control over the FRET rate for its wide range of applications. Hence, enhancing the FRET rate using different techniques has been extensively studied in the literature. Research indicates that introducing additional particles to a system consisting of a donor-acceptor pair can change the behaviour of FRET in the system. One such technique is to utilize the collective oscillations of the surface electrons of a neighboring electrically-neutral metal nanoparticle (MNP). However, the perceived changes on the FRET rate between the donor and the acceptor, when the MNP carries excess electrical charges are yet unknown. In this paper, we study these changes by introducing a charged MNP, in the proximity of an excited donor and a ground state acceptor. We deploy the classical Green's tensor to express the FRET rate in the system. We consider an effective dielectric response for the MNP, which accounts for the extraneous surface charge effects. We analyze the electrical potential at the acceptor position due to the changed dipole moment of the donor molecule as a result of the electric field induced at the donor position, and obtain the FRET rate of the system. This model considers arbitrary locations and orientations of the two molecular dipole moments with regard to the position of the spherical MNP. We present the enhancement of the FRET rate, predominantly caused by both the surface plasmon excitations and the extraneous surface electrical charges carried by the neighboring MNP. We obtain the results by varying the separation distance between the molecules and the MNP, the transition frequency of the donor-acceptor pair and the size of the metallic sphere. Specifically, we demonstrate that a donor-acceptor pair placed in the vicinity of an electrically-charged Silver MNP exhibits a remarkable improvement in the FRET rate. Furthermore, the aggregate FRET enhancement is determined by other characteristics such as the location of the donor, transition frequency, separation distances and the radius of the MNP. In essence, these findings reveal an approach to realize the enhanced FRET rate in a larger span in a more controlled manner that is desirable in many FRET-based applications including spectroscopic measurements

The RNA-Binding Protein Musashi1 Affects Medulloblastoma Growth via a Network of Cancer- Related Genes and Is an Indicator of Poor Prognosis

Musashi1 (Msi1) is a highly conserved RNA-binding protein that is required during the development of the nervous system. Msi1 has been characterized as a stem cell marker, controlling the balance between self-renewal and differentiation, and has also been implicated in tumorigenesis, being highly expressed in multiple tumor types. We analyzed Msi1 expression in a large cohort of medulloblastoma samples and found that Msi1 is highly expressed in tumor tissue compared with normal cerebellum. Notably, high Msi1 expression levels proved to be a sign of poor prognosis. Msi1 expression was determined to be particularly high in molecular subgroups 3 and 4 of medulloblastoma. We determined that Msi1 is required for tumorigenesis because inhibition of Msi1 expression by small-interfering RNAs reduced the growth of Daoy medulloblastoma cells in xenografts. To characterize the participation of Msi1 in medulloblastoma, we conducted different high-throughput analyses. Ribonucleoprotein immunoprecipitation followed by microarray analysis (RIP-chip) was used to identify mRNA species preferentially associated with Msi1 protein in Daoy cells. We also used cluster analysis to identify genes with similar or opposite expression patterns to Msi1 in our medulloblastoma cohort. A network study identified RAC1, CTGF, SDCBP, SRC, PRL, and SHC1 as major nodes of an Msi1-associated network. Our results suggest that Msi1 functions as a regulator of multiple processes in medulloblastoma formation and could become an important therapeutic target

Downregulation of Cinnamyl-Alcohol Dehydrogenase in Switchgrass by RNA Silencing Results in Enhanced Glucose Release after Cellulase Treatment

Cinnamyl alcohol dehydrogenase (CAD) catalyzes the last step in monolignol biosynthesis and genetic evidence indicates CAD deficiency in grasses both decreases overall lignin, alters lignin structure and increases enzymatic recovery of sugars. To ascertain the effect of CAD downregulation in switchgrass, RNA mediated silencing of CAD was induced through Agrobacterium mediated transformation of cv. “Alamo” with an inverted repeat construct containing a fragment derived from the coding sequence of PviCAD2. The resulting primary transformants accumulated less CAD RNA transcript and protein than control transformants and were demonstrated to be stably transformed with between 1 and 5 copies of the T-DNA. CAD activity against coniferaldehyde, and sinapaldehyde in stems of silenced lines was significantly reduced as was overall lignin and cutin. Glucose release from ground samples pretreated with ammonium hydroxide and digested with cellulases was greater than in control transformants. When stained with the lignin and cutin specific stain phloroglucinol-HCl the staining intensity of one line indicated greater incorporation of hydroxycinnamyl aldehydes in the lignin