Evaluating the Effects of Bisphenols F and S with Respect to Bisphenol A on Primordial Germ Cell Migration in Zebrafish (Danio rerio) Embryos Using Immunofluorescence Microscopy

Primordial Germ Cell (PGC) migration occurs in early embryonic development and is highly conserved across taxa. PGC migration occurs within the first 24 hours post fertilization (hpf) in zebrafish, making the organism an efficient model for observing the migration pathway. Proper PGC migration is necessary for normal gonad development and, in some species, sex determination. Disruption of this process leads to defects in gonad formation and abnormal sex determination and differentiation. Studies show that endocrine-disrupting chemicals such as bisphenol A (BPA) disrupt PGC migration in zebrafish. BPA is an estrogenic compound that has been linked to a variety of human diseases, including various cancers, diabetes, reproductive disorders, obesity, and cardiovascular diseases. It is one of the most widely used synthetic compounds worldwide, as it is used to make polycarbonate plastics. Many studies provide evidence of the harmful effects of BPA on living organisms. In response, manufacturers have started to use replacements such as bisphenol F (BPF) and bisphenol S (BPS). However, due to their structural similarity, it is likely that BPF and BPS are just as harmful to organisms as BPA. In this study, we use antibody staining and immunofluorescence microscopy to confirm that BPA exposure results in abnormal PGC migration in zebrafish embryos, as previously studied, and to illustrate that BPF and BPS exposure results in similar PGC migration defects

Pathways from parental adverse childhood experiences to child emotion regulation: The role of parent emotion regulation and emotion socialization

The present study evaluates the mediating role of parent emotion regulation (ER) and parent emotion-related socialization behaviors (ERSBs) in the relation between parent adverse childhood experiences (ACEs) and child ER. Caregivers of children ages 2 through 5 (inclusive) completed traditional and expanded ACEs scales, the Difficulties in Emotion Regulation Scale Short Form, the Coping with Toddlers’ Negative Emotions Scale, and the Emotion Regulation Checklist. Data analysis involved correlation and mediation analyses. Parent difficulties in ER statistically mediated the association between parent ACEs and child ER such that a higher expanded ACEs score was associated with more parent difficulties in ER, and these difficulties were related to lower child ER. Although parent ER and supportive ERSBs independently contribute to child ER, data did not support a mediational role for ERSB or sequential mediation. Findings suggest that parent ER may be one avenue for the reduction of intergenerational transmission of trauma

Transcription of nanos-1 in Zebrafish Embryos is not Affected by Bisphenol A: Evaluated Using Quantitative Real-Time PCR

The presence of primordial germ cells (PGCs) is crucial for proper gonad formation in zebrafish (Danio rerio). The many aspects of PGC migration that allow these cells to reach the proper location at the gonadal ridge include receptors, ligands, germ plasm components, and internal maintenance of PGCs. Any one of these factors could be affected by endocrine-disrupting chemicals (EDCs), which have been shown to alter the directed migration of these cells during early embryonic development. Based on recent research wherein the EDC bisphenol A (BPA) inhibited normal PGC migration, we have used the same dose of BPA to determine the impact of BPA on a gene central to proper germ cell migration. Zebrafish embryos were exposed to BPA, and the levels of the target gene nanos-1 were analyzed using quantitative real-time PCR (q-PCR). The target gene nanos-1 is a critically important germplasm component that allows for survival and proper migration of PGCs. The q-PCR results showed that BPA did not affect the transcription level of nanos-1 in zebrafish embryos

Evaluation of normal findings using a detailed and focused technique for transcutaneous abdominal ultrasonography in the horse

Background Ultrasonography is an important diagnostic tool in the investigation of abdominal disease in the horse. Several factors may affect the ability to image different structures within the abdomen. The aim of the study was to describe the repeatability of identification of abdominal structures in normal horses using a detailed ultrasonographic examination technique and using a focused, limited preparation technique. Methods A detailed abdominal ultrasound examination was performed in five normal horses, repeated on five occasions (total of 25 examinations). The abdomen was divided into ten different imaging sites, and structures identified in each site were recorded. Five imaging sites were then selected for a single focused ultrasound examination in 20 normal horses. Limited patient preparation was performed. Structures were recorded as ‘identified’ if ultrasonographic features could be distinguished. The location of organs and their frequency of identification were recorded. Data from both phases were analysed to determine repeatability of identification of structures in each examination (irrespective of imaging site), and for each imaging site. Results Caecum, colon, spleen, liver and right kidney were repeatably identified using the detailed technique, and had defined locations. Large colon and right kidney were identified in 100% of examinations with both techniques. Liver, spleen, caecum, duodenum and other small intestine were identified more frequently with the detailed examination. Small intestine was most frequently identified in the ventral abdomen, its identification varied markedly within and between horses, and required repeated examinations in some horses. Left kidney could not be identified in every horse using either technique. Sacculated colon was identified in all ventral sites, and was infrequently identified in dorsal sites. Conclusions Caecum, sacculated large intestine, spleen, liver and right kidney were consistently identified with both techniques. There were some normal variations which should be considered when interpreting ultrasonographic findings in clinical cases: left kidney was not always identified, sacculated colon was occasionally identified in dorsal flank sites. Multiple imaging sites and repeated examinations may be required to identify small intestine. A focused examination identified most key structures, but has some limitations compared to a detailed examination

Rich potential in adult literacy: Lost in an era of deep economic recession

Abstract Classes in England for students of adult literacy and English for Speakers of Other Languages (ESOL) have been severely cut back in the past seven years. Those that remain are run on the basis that the class can only be viable through accreditation if it meets current funding targets within specified time periods, reducing many schemes to crash courses in employability. Adult education had been cut by 40% by 2015 alone. The overt purpose of literacy classes is now rooted in functional skills English syllabi, built on the assumption that students attend courses for qualifications. There is not enough known about why the students themselves – both ESOL and native speakers – are motivated to come forward for classes. In this research, an experienced adult literacy/ESOL teacher ran 26 in-depth interviews with students and teachers in four organisations in South London and two in Birmingham to find out what they valued about the classes they were attending at the time, other than obtaining qualifications. A social practices approach is used to examine students’ motives for improving their literacy, alongside discussions of learning theory, and an auto-ethnographic approach is used throughout the dissertation. Findings include a very broad range of reasons for why students are in class and what they gain from learning that enhances their everyday lives. Insight is provided into how students use technology as part of everyday life. It was discovered that many respondents need to continue to learn as long as possible. These results are considered in the light of globalisation studies, learning theory, ‘vulnerability’ theory and multicultural studies of superdiversity

Giving Voice to the Voiceless: Incorporating Nonhuman Animal Perspectives as Journalistic Sources

As part of journalism\u27s commitment to truth and justice by providing a diversity of relevant points of view, journalists have an obligation to provide the perspective of nonhuman animals in everyday stories that influence the animals\u27 and our lives. This essay provides justification and guidance on why and how this can be accomplished, recommending that, when writing about nonhuman animals or issues, journalists should: 1) observe, listen to, and communicate with animals and convey this information to audiences via detailed descriptions and audiovisual media, 2) interpret nonhuman animal behavior and communication to provide context and meaning, and 3) incorporate the animals’ stories and perspectives, and consider what is in their best interest. To fairly balance animal-industry sources and the anthropocentric biases that are traditionally inherent in news requires that journalists select less objectifying language and more appropriate human sources without a vested interest in how animals are used

Do colored cells in risk matrices affect decision-making and risk perception? Insights from randomized controlled studies

Risk matrices communicate the likelihood and potential impact of risks and are often used to inform decision-making around risk mitigations. The merits and demerits of risk matrices in general have been discussed extensively, yet little attention has been paid to the potential influence of color in risk matrices on their users. We draw from fuzzy-trace theory and hypothesize that when color is present, individuals are likely to place greater value on reducing risks that cross color boundaries (i.e., the boundary-crossing effect), leading to sub-optimal decision making. In two randomized controlled studies, employing forced-choice and willingness-to-pay measures to investigate the boundary-crossing effect in two different color formats for risk matrices, we find preliminary evidence to support our hypotheses that color can influence decision making. The evidence also suggests that the boundary-crossing effect is only present in, or is stronger for, higher numeracy individuals. We therefore recommend that designers should consider avoiding color in risk matrices, particularly in situations where these are likely to be used by highly numerate individuals, if the communication goal is to inform in an unbiased way