Differential requirement for BMP signaling in atrial and ventricular lineages establishes cardiac chamber proportionality

AbstractThe function of an organ relies upon the proper relative proportions of its individual operational components. For example, effective embryonic circulation requires the appropriate relative sizes of each of the distinct pumps created by the atrial and ventricular cardiac chambers. Although the differences between atrial and ventricular cardiomyocytes are well established, little is known about the mechanisms regulating production of proportional numbers of each cell type. We find that mutation of the zebrafish type I BMP receptor gene alk8 causes reduction of atrial size without affecting the ventricle. Loss of atrial tissue is evident in the lateral mesoderm prior to heart tube formation and results from the inhibition of BMP signaling during cardiac progenitor specification stages. Comparison of the effects of decreased and increased BMP signaling further demonstrates that atrial cardiomyocyte production correlates with levels of BMP signaling while ventricular cardiomyocyte production is less susceptible to manipulation of BMP signaling. Additionally, mosaic analysis provides evidence for a cell-autonomous requirement for BMP signaling during cardiomyocyte formation and chamber fate assignment. Together, our studies uncover a new role for BMP signaling in the regulation of chamber size, supporting a model in which differential reception of cardiac inductive signals establishes chamber proportion

Too young to correct: A developmental test of the three-stage model of social inference

The 3-stage model of social inference posits that people categorize behaviors and characterize actors or situations effortlessly, but correct these characterizations with additional information effortfully. The current paper tests this model using developmental data, assuming that the less cognitively demanding processes in the model (i.e., categorization, characterization) should appear earlier in development, whereas the more demanding correction process, should not appear until later in development. Using two different paradigms, Studies 1 and 3 found that younger children failed to take situational information into account while characterizing the actor. Study 2 found that younger children failed to take dispositional information into account while characterizing the situation. In contrast, in these three studies older children used the available information to correct their characterizations of the actors and of the situations. Consistently with the 3-stage model, during elementary school years, children start to integrate additional information when drawing explicit social inferences. In Study 4, children of all age-levels used a prior expectancy to draw a dispositional inference, ignoring situational information, suggesting that characterizations based on prior expectancies about an actor are a highly efficient process, not contemplated by the model. The 4 studies together illustrate how developmental data can be valuably used to test adult socio-cognitive models, to extend their validity, or simply further inform those models.info:eu-repo/semantics/publishedVersio

Reiterative roles for FGF signaling in the establishment of size and proportion of the zebrafish heart

AbstractDevelopment of a functional organ requires the establishment of its proper size as well as the establishment of the relative proportions of its individual components. In the zebrafish heart, organ size and proportion depend heavily on the number of cells in each of its two major chambers, the ventricle and the atrium. Heart size and chamber proportionality are both affected in zebrafish fgf8 mutants. To determine when and how FGF signaling influences these characteristics, we examined the effect of temporally controlled pathway inhibition. During cardiac specification, reduction of FGF signaling inhibits formation of both ventricular and atrial cardiomyocytes, with a stronger impact on ventricular cells. After cardiomyocyte differentiation begins, reduction of FGF signaling can still result in a deficiency of ventricular cardiomyocytes. Consistent with two temporally distinct roles for FGF, we find that increased FGF signaling induces a cardiomyocyte surplus only before cardiac differentiation begins. Thus, FGF signaling first regulates heart size and chamber proportionality during cardiac specification and later refines ventricular proportion by regulating cell number after the onset of differentiation. Together, our data demonstrate that a single signaling pathway can act reiteratively to coordinate organ size and proportion

Carbon dioxide emissions and heavy metal contamination analysis of stone mastic asphalt mixtures produced with high rates of different waste materials

The incorporation of waste materials in asphalt mixtures has driven several studies mainly focused on improving their mechanical performance while minimizing the use of virgin materials. However, these could only be considered cleaner solutions for road paving works if their production and application do not present additional risks for human health and the environment. Therefore, this study aims at assessing the carbon dioxide emissions and possible leachates of stone mastic asphalt mixtures produced with high rates of different waste materials for binder modification or material recycling. Thus, a chemical analysis of eluates that were in contact with those mixtures and an estimation of the carbon dioxide emissions associated with their production and transportation were carried out under different scenarios. In conclusion, these mixtures comply with the established specifications for hazardous leachates. The addition of waste materials to these mixtures decreases carbon dioxide emissions, especially for recycled stone mastic asphalt mixtures with 50% reclaimed asphalt pavement material and bitumens modified with waste materials. Thus, the studied mixtures are innovative solutions for future use in pavement maintenance and rehabilitation operations, in line with the circular economy concept.The authors gratefully acknowledge the funding by the Portuguese Government and EU/FSE within a Portuguese Foundation for Science and Technology (FCT) Ph.D. grant (SFRH/BD98379/2013), in the scope of POPH/QREN

Mechanical, surface and environmental evaluation of stone mastic asphalt mixtures with advanced asphalt binders using waste materials

The reuse of waste materials in asphalt mixtures has been recently investigated, in order to develop new sustainable solutions for the road-paving industry. Such materials should improve the mechanical performance and provide safe/comfortable pavement surface courses for road users, without compromising their environmental performance. Thus, the aim of this study is to evaluate the mechanical, surface and environmental properties of stone mastic asphalt (SMA) mixtures produced with forward-looking asphalt binders incorporating waste materials. These binders were designed to maximise the waste material content using motor oil, high-density polyethylene, styrene?butadiene?styrene and crumb rubber, while performing so well as a commercial modified bitumen. Finally, the overall performance of the SMA mixtures produced with the selected binders was evaluated. It was concluded that these mixtures improve the water sensitivity, fatigue cracking and permanent deformation performance. The requirements for macrotexture, skid resistance and presence of heavy metals in leachates of these mixtures were similarly fulfilled. Thus, this work shows that new asphalt mixtures with waste materials can be used in road-paving works to improve the performance without compromising human and environmental safety.Portuguese Government and EU/FSE within a PhD fellowship (SFRH/BD98379/2013) of the FCT, in the scope of POPH/QREN, by ERDF (European Regional Development Fund) funds through the Competitiveness Factors Operational Programme (COMPETE)info:eu-repo/semantics/publishedVersio

Effects of Green Tea Marinade in the Bioaccessibility of Tonalide and Benzophenone 3 in Cooked European Seabass

The authors acknowledgment Fundação para a Ciência e Tecnologia: NORTE-01-0145-FEDER-000041; IM acknowledges FCT for Ph.D. Grant DFA/BD/4413/2020. Publisher Copyright: © 2022 by the authors.Fish consumption is an indicator of human exposure to personal care products (PCP) such as tonalide (AHTN) and benzophenone 3 (BP3). Although most fish consumed is cooked, the impact of cooking procedures on PCP levels is difficult to evaluate. Hence, the aim of this work was to provide thorough information on the stability and bioaccessibility of AHTN and BP3 upon cooking and in vitro digestion. A green tea (Camellia sinensis) marinade, rich in polyphenol, was used as mitigating strategy to reduce these contaminants. Roasting and frying reduced AHTN and BP3 levels in European seabass (Dicentrarchus labrax) spiked samples. Additionally, the green tea marinade promoted a reduction of up to 47% AHTN and 35% BP3. Bioaccessibility of AHTN was higher (up to 45%), and increased with the use of green tea marinades. BP3 showed a bioaccessibility below 19% in all cooked samples. Overall, a decrease in PCP levels was observed after cooking; this decrease was even more pronounced when marination was previously used. However, this decrease is cancelled out by the fact that the bioaccessible fraction of the contaminants increases in an inverse way; therefore, none of these processes can be considered a mitigating alternative.publishersversionpublishe

Controlled polymerisation and purification of branched poly(lactic acid) surfactants in supercritical carbon dioxide

Product degradability, sustainability and low-toxicity are driving demand for the synthesis of biobased polymers and surfactants. Here we report the synthesis of novel surface active polymers using cyclic esters (D,L-lactide) and temperature sensitive polyols (D-sorbitol) as renewable building blocks. We highlight the modification of chain length and degree of branching to provide a route to tailoring the properties and application performance of these new compounds. High processing temperatures (≥180 °C) and harsh post-reaction treatments are often needed to remove residual monomer and catalysts and these can become barriers to creating materials based on renewable resources. Here we exploit supercritical carbon dioxide (scCO2) as a green solvent to overcome these challenges; significantly reducing reaction temperatures, targeting controlled molecular weights with narrow dispersities and reducing sideproduct formation. Additionally in the same pot, we can use supercritical extraction to purify the compounds and to efficiently remove unreacted reagents, which could be recovered and recycled. We believe that our approach to the production and purification of these novel branched poly(lactides) is a significant step towards the development of the next generation of biopolymers and green surfactants, combining both the use of bio-sourced raw materials and the potential to use sustainable, low energy processes and techniques

Strategies for the hypothermic preservation of cell sheets of human adipose stem cells

Cell Sheet (CS) Engineering is a regenerative medicine strategy proposed for the treatment of injured or diseased organs and tissues. In fact, several clinical trials are underway using CS-based methodologies. However, the clinical application of such cell-based methodologies poses several challenges related with the preservation of CS structure and function from the fabrication site to the bedside. Pausing cells at hypothermic temperatures has been suggested as a valuable method for short-term cell preservation. In this study, we tested the efficiency of two preservation strategies, one using culture medium supplementation with Rokepie and the other using the preservation solution Hypothermosol, in preserving human adipose stromal/stem cells (hASC) CS-like confluent cultures at 4°C, during 3 and 7 days. Both preservation strategies demonstrated excellent ability to preserve cell function during the first 3 days in hypothermia, as demonstrated by metabolic activity results and assessment of extracellular matrix integrity and differentiation potential. At the end of the 7th day of hypothermic incubation, the decrease in cell metabolic activity was more evident for all conditions. Nonetheless, hASC incubated with Rokepie and Hypothermosol retained a higher metabolic activity and extracellular matrix integrity in comparison with unsupplemented cells. Differentiation results for the later time point showed that supplementation with both Rokepie and Hypothermosol rescued adipogenic differentiation potential but only Rokepie was able to preserve hASC osteogenic potential.This work was supported by: SF-R by PhD grant PD/BD/135252/2017; AFC by contract financed by SFRH/BPD/109595/2015; MTC by NORTE-01-0145-FEDER-000021 and RPP by IF/ 00347/2015, all supported by Fundac¸ão para a Ciencia e Tecnologia (FCT). This work was supported by LA ICVS/3B’s project UID/Multi/ 50026/2013 (POCI-01-0145-FEDER-007038), financed by FCT and Fundo Europeu de Desenvolvimento Regional (FEDER), as well as Gene2Skin Project (H2020-TWINN-2015-692221). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript

Occurrence and seasonal variation of several endocrine disruptor compounds (pesticides, bisphenols, musks and UV-filters) in water and sediments from the estuaries of Tagus and Douro Rivers (NE Atlantic Ocean coast)

Funding Information: This work was supported by FEDER (Programa Operacional Competitividade e Internacionalização - COMPETE 2020), from PIDDAC through FCT/MCTES project POCI-01-0145-FEDER-028708-PTDC/ASP-PES/28708/2017 , by UIDB/04423/2020 and AgriFood XXI R&D&I project, operation No. NORTE-01-0145-FEDER-000041 , co-financed by the European Regional Development Fund (ERDF) through NORTH 2020 ( Northern Regional Operational Program 2014/2020 ). The Portuguese Foundation for Science and Technology (FCT) supported the contract of Sara C. Cunha (IF/01616/2015), the Ph.D. Grant of Isa Marmelo (DFA/BD/4413/2020) and the contract of Patrícia Anacleto (CEECIND/01739/2017). Other support was provided by the Laboratory of Ecotoxicology and Ecology, Department of Population Studies of the Institute of Biomedical Sciences of Abel Salazar (ICBAS) of the University of Porto , and by the Strategic Funding UIDB/Multi/04423/2019 , UIDB/04423/2020 and LA/P/0101/2020 through national funds provided by FCT and ERDF in the framework of the programme Portugal 2020 to CIIMAR. Publisher Copyright: © 2022 Elsevier B.V.Exposure of aquatic environments to emerging contaminants is a global issue, special relevant in many estuaries due to impacts from anthropogenic activity. The aim of this work was to evaluate thirty-seven endocrine disruptor chemicals (EDCs) from four different classes (pesticides, bisphenols, polycyclic musks and UV-filters) in water and sediment samples collected during one-year in the estuaries of Tagus and Douro Rivers located into the NE Atlantic Ocean coast. EDCs analysis was achieved afterward validation of a gas-chromatography mass spectrometry (GC–MS) method using Dispersive Liquid-Liquid Microextraction (DLLME) as extraction procedure for water samples, and Quick, Easy, Cheap, Efficient, Rugged and Safe (QuEChERS) combined with DLLME for sediments. Tagus estuary presented higher levels of contamination with pesticide residues and bisphenols (BPs) than the Douro estuary in both water and sediment samples. Contrariwise, levels and frequency of polycyclic musks (PCMs) and UV-filters (UVF) were slightly higher in Douro estuary. Levels of pesticide residues in both sediment and water samples, and levels of PCMs and UVF in water samples were higher in warmer seasons (summer and spring) than in colder ones (winter and autumn). The opposite was found in what respect levels of BPs in water and sediment samples, and PCMs and UVF levels in sediment samples. Although the levels found for each contaminant are low, usually in the order of a few ng/mL(g), the presence of a high number of toxic compounds is a source of concern and requires constant monitoring.publishersversionpublishe

Integrin-specific hydrogels for growth factor-free vasculogenesis

Integrin-binding biomaterials have been extensively evaluated for their capacity to enable de novo formation of capillary-like structures/vessels, ultimately supporting neovascularization in vivo. Yet, the role of integrins as vascular initiators in engineered materials is still not well understood. Here, we show that αvβ3 integrin-specific 3D matrices were able to retain PECAM1+ cells from the stromal vascular fraction (SVF) of adipose tissue, triggering vasculogenesis in vitro in the absence of extrinsic growth factors. Our results suggest that αvβ3-RGD-driven signaling in the formation of capillary-like structures prevents the activation of the caspase 8 pathway and activates the FAK/paxillin pathway, both responsible for endothelial cells (ECs) survival and migration. We also show that prevascularized αvβ3 integrin-specific constructs inosculate with the host vascular system fostering in vivo neovascularization. Overall, this work demonstrates the ability of the biomaterial to trigger vasculogenesis in an integrin-specific manner, by activating essential pathways for EC survival and migration within a self-regulatory growth factor microenvironment. This strategy represents an improvement to current vascularization routes for Tissue Engineering constructs, potentially enhancing their clinical applicability.The authors would like to acknowledge the financial support from the Consolidator Grant “ECM_INK” (ERC-2016-COG-726061) and the Starting Grant “CapBed” (ERC2018-STG-805411), to the FSE/POCH (Fundo Social Europeu através do Programa Operacional do Capital Humano) under the scope of the PD/169/2013, NORTE-08- 5369-FSE-000037 (H.R.M.), and to FCT/MCTES (Fundação para a Ciência e a Tecnologia/ Ministério da Ciência, Tecnologia, e Ensino Superior) through the grants SFRH/BD/119756/2016 (D.B.R.), Ph.D. grant PD/BD/135252/2017 (S.F.R.) and IF/00347/ 2015 (R.P.P.)