Evaluation of PECAM-1 Gene Polymorphism in Patients with Periodontal Disease and Healthy Individuals

Objective. Our aim in this paper was to investigate the possible genetic association between three Ser563Asn, Leu125Val and Arg670Gly polymorphisms of the PECAM-1 gene and periodontitis. Methods. Genomic DNA was isolated from whole blood of 105 periodontal patient (52 with chronic periodontitis and 53 with aggressive periodontitis) and 101 healthy individuals. Samples were genotyped and analyzed for the three single-nucleotide polymorphisms (SNPs) of PECAM-1 using polymerase chain reaction with sequence-specific primers (PCR-SSPs). Results. A statistically significant difference was found between the genotypic distribution of the Ser563Asn polymorphism in patients with periodontitis compared to controls (P = 0.02). But there were no statistically significant difference between the allele frequencies in the different groups (P = 0.05). The other two polymorphisms did not show a statistically significant difference in their allele and genotype frequencies between the groups. There was no statistically significant difference found for any of the polymorphisms allele and genotype distribution in aggressive and chronic periodontitis either. Conclusions. No significant association was found between the polymorphism tested and the subgroups of periodontitis, further research is still necessary to determine whether this polymorphism can be used as a genetic marker of periodontitis

The Effects of Different Ionic Liquid Coatings and the Length of Alkyl Chain on Antimicrobial and Cytotoxic Properties of Silver Nanoparticles

Introduction: The antibacterial efficacy and toxicity of silver nanoparticles (AgNPs) depends on their physicochemical properties including size, shape, surface charge and surface coatings. The Objectives of this study were: i) To synthesize and characterize positively charged AgNPs coated by different ionic-liquids with different alkyl chain lengths, ii) To evaluate the antimicrobial activity of these nanoparticles against Enterococcus faecalis compared to sodium hypochlorite (NaOCl) and chlorhexidine (CHX), iii) To compare the cytocompatibility of these solutions against L929 mouse fibroblasts. Methods and Materials: AgNPs with positive surface charges capped by two different ionic liquids [imidazolium (Im) and pyridinium (Py)] with two alkyl chain lengths (C12 and C18) were synthesized. Im and Py were also tested as control groups. The characterization revealed synthesis of spherical NPs in the size range of 6.7-18.5 nm with a surface charge ranging from +25 to +58 mV. To standardize the comparisons, the surface charge to radius ratio of each nanoparticle was calculated. The minimum inhibitory concentrations (MIC) of the AgNP solutions, NaOCl and CHX were determined against E. faecalis by a microdilution test. An MTT-based cytotoxicity assay evaluated the cytotoxicity of the solutions in different concentrations on L929 fibroblasts. One-way and two-way ANOVA were used for statistical analysis. Results: All tested AgNPs reached MIC90 in significantly lower concentrations compared to CHX and NaOCl. C12 Py-coated AgNPs had the lowest MIC90 value. CHX and NaOCl were more toxic on fibroblasts than all tested AgNPs. Im-coated AgNPs had better compatibility with fibroblasts than Py-coated particles; and C12 Im AgNPs had the best biocompatibility. Variations in alkyl chain length had no effects on the biocompatibility of AgNPs. Conclusion: Py improved the antibacterial efficacy of AgNPs compared to Im; however, it had a negative effect on cytocompatibility. Alkyl chain length had no effects on AgNPs’ bioactivity.Keywords: Antibacterial Agents; Chlorhexidine; Cytotoxicity; Metal Nanoparticles; Sodium Hypochlorit

Qajar Bonapartism: The Model of State–Class Relationship in Qajar Iran

IntroductionThe present research deals with the discrepancy between the hypothesized theory of the Asiatic mode of production and the empirical evidence from the Qajar era. Specifically, it explored whether the state–class relationship in the Qajar era can accurately be characterized as oriental despotism (or an Asian state), or it reflects a state where the power of the king was limited by influential classes, such as the clergy, affluent merchants, local nobility, provincial rulers, princes, large landowners, and tribal chiefs. The central focus of this research was to determine whether the Qajar state enjoyed power concentration or operated within a kind of power plurality. In this respect, the central question is: Was the power of the Qajar state limited by social classes, or did it wield absolute and supra-class authority?Theoretical FrameworkThe study examined the state–class relationship through the lens of Karl Marx’s theory of the state. Marx’s perspective on the state can be categorized into three distinct models: the powerless state, the state with relative power, and the state with absolute power. Applying these three models, the present study analyzed the dynamics of the state–class relationship during the Qajar era.According to Marx’s instrumentalist theory, the concept of a class state suggests that both the form and essence of the state are contingent upon prevailing classes. While the state may exhibit diverse variations and characteristics in different historical contexts, it fundamentally relies on classes. In all instances, the state functions as a dependent entity and a tool of the ruling class. Marx presented his theory of the Bonapartist state in The Eighteenth Brumaire of Louis Bonaparte (1852) and The Class Struggles in France 1848-1850 (1850). Marx acknowledges that the state possesses a certain degree of independent power or relative autonomy from the ruling class. According to Marx and Engels, the absence of private land ownership and the existence of large-scale state-controlled water facilities, despite their apparent contradiction, are the defining characteristics of the Asian state, in which the autocratic state machinery exercises control over the production surplus and serves not only as the central apparatus for oppression but also as a tool for economic exploitation at the disposal of the ruling class. In such a setting, “the state reigns supreme.”As evident, Marx’s triadic model of the state analyzes the state–class relationship at three levels. The model of class state portrays a state that lacks power and relative autonomy from the ruling class. The Bonapartist state enjoys relative autonomy from the ruling class, while the Asian state wields absolute power and autocratic authority over all societal classes. The present research used Marx’s triadic model as the analytical framework to examine the state–class relationship during the Qajar era.Materials and Methods This research employed a historical case study approach, which involves gathering extensive information through various data collection methods over an extended temporal span. The collected information is systematically analyzed with the explicit objective of deriving theoretical insights. The documentary research method within the framework of recorded or written history was used to collect the relevant information. There are three strategies for data analysis: pattern matching, explanation building, and time series analysis. Given the descriptive nature of the current case study, the pattern matching method, specifically the type of rival explanations, was utilized. This method involves comparing the historical pattern derived from experience with the predicted theoretical pattern.Results and DiscussionThe central question addressed in the present article pertains to the extent of the Qajar state’s power; whether it was constrained by social classes or characterized by absolute and supra-class authority. According to the research findings, the influential clerics, relying on their social support base, exerted their influence over the state. This influence manifested openly through the issuance of fatwas in significant events such as the Russo-Persian Wars, the Persian Tobacco Protest, or the Constitutional Movement. Furthermore, the clerics often succeeded in establishing common interests through their relationships with statesmen, thereby exerting influence over high-ranking state officials. Notably, clerics comprised 20% of the social composition of the first parliament, which signifies their official entry into the power structure of the time.Prominent and affluent merchants, particularly in the first half of the 19th century, wielded influence by fulfilling the financial requirements of the state and cooperating closely with it. However, their role evolved in the second half of the 19th century marked by events like the Tobacco Protests and Monsieur Naus, when they joined the protesters and disrupted the country’s economic cycles due to conflicting interests. This class emerged as one of the most influential groups in Iran during the Qajar era. With the establishment of the Constituent Assembly, they secured a significant one-third of the parliament composition.The influential patriarchs of tribes and the heads of important clans held significant sway due to their independent geographic position and economic resources, military strength, and provision of manpower to the Qajar army. This enabled them to exert influence and even engage in direct conflicts with the state, such as during the Constitutional Movement.Given Iran’s population structure, which predominantly comprised farmers, the large landowners assumed the role of quasi-sovereigns within the territories under their ownership. Their possession of extensive estates, personal military forces, and substantial incomes derived from landownership, combined with a weak bureaucracy and an inefficient tax system, granted them considerable autonomy in areas under their influence.ConclusionAccording to the findings, it becomes highly challenging to conceive of the Qajar state as the entity possessing absolute power, as Marx suggests as the primary characteristic of the Asian state. In the Qajar Iran, influential social classes, including the clergy, affluent merchants, local nobility, provincial rulers, princes, large landowners, and tribal chiefs, served as intermediary layers that limited the state’s power and prevented the establishment of a supreme master or an absolute ruler. Moreover, the Qajar state, originating from the Qajar tribe, was not practically reliant on the tribe itself or other social classes, so the Qajar state actually employed various methods, such as granting state positions or making discord between tribes, to control and even suppress them. Accordingly, the Qajar state cannot be categorized as a mere instrument of the ruling class or an entity with absolute power. It does not align with the concept of a class state or even a supra-class state. Instead, enjoying power and relative autonomy from the dominant class, the Qajar state could create a relative balance between social forces, leading to its characterization as a Bonapartist state

Improved delivery of voriconazole to Aspergillus fumigatus through solid lipid nanoparticles as an effective carrier

Novel voriconazole-loaded solid lipid nanoparticles (VRC-SLNs) were prepared via probe-ultrasonication method, and the resultant nanoparticles were tested on A. fumigatus. Voriconazole-loaded solid lipid nanoparticles were prepared using the probe ultrasonication technique. Photon correlation spectroscopy (PCS) was used to determine the average particle size and zeta potential of SLN formulations. Transmission electron microscopy was also used to determine the morphology of solid lipid nanoparticles. To determine MIC for all SLN formulations against strains of Aspergillus the Clinical and Laboratory Standards Institute guidelines was followed. The results showed that SLNs containing voriconazole exhibited almost spherical shape with a diameter and zeta potential of 286.6 ± 4.7 nm and −15 ± 4.1 mV respectively. This novel formulation of VRC led to a significant reduction in MICs for all Aspergillus either VRC-susceptible or VRC-resistant isolates (P < 0.05). The MIC50 drug concentration was obtained as 0.015 μg/ml for both VRC-susceptible strains of A. fumigates while it was 0.25 μg/ml against VRC (p < 0.05). VRC-resistant strains showed a MIC50 of 0.015 μg/ml as well. These novel drug formulations may increase the bioavailability through an increase in the dissolution rate of voriconazole. This study showed, for the first time, VRC-SLNs can be employed as an effective delivery systems for VRC on A. fumigatus isolates

Effects of Early Mobilization Protocol on Cognitive Outcome after Cardiac Surgery

Background: This study aimed at determining the effects of implementation of “early mobilization protocol” on incidence of cognitive dysfunction after cardiac surgery. Methods: In a randomized controlled trial, 80 adult patients, who had undergone elective cardiac surgery were randomly assigned to intervention (early mobilization protocol; n = 40) and control (routine physical therapy; n = 40) groups. Early mobilization was initiated from the first post-op morning and continued until discharge from the ICU. Cognitive dysfunction was assessed by the mini mental state examination (MMSE) questionnaire. The MMSE questionnaire was completed at three occasions for every patient: one day before surgery, second post-op day, and at the time of discharge from the intensive care unit (ICU). Results: Preoperative cognitive status had no difference between the two groups (P = 0.310). Post-op cognitive dysfunction was significantly more commonly reported in the control group. The MMSE scores were higher in early mobilized patients compared to the control group on the first post-op day (median: 28; inter quartile range: 26 to 30 versus median: 25; IQR: 22 to 27; p = 0.001) and at the time of discharge from the ICU (median: 29; IQR: 28 to 30 versus median: 26; IQR: 25 to 28; p = 0.001). In multivariate analysis, duration of tracheal intubation and “early mobilization protocol” had significant effects on patients’ length of ICU stay. Conclusions: Implementation of early mobilization protocol has positive effects on cognitive outcome and ICU stay after cardiac surgery

The clinical significance of biliary findings in magnetic resonance enterography of patients with inflammatory bowel disease

Purpose: Given the association of inflammatory bowel disease (IBD) and primary sclerosing cholangitis (PSC), we aimed to investigate the clinical relevance of abnormal hepatobiliary findings on magnetic resonance enterography (MRE) of IBD patients considering the risk of over- or underestimation of PSC at MRE. Material and methods: Using the MRE dataset of patients referring to a tertiary hospital and the National Registry of Crohn’s and Colitis, 69 MREs, including 23 IBD-PSC, 23 IBD-without PSC, and 23 healthy controls (HC), were retrospectively reviewed by 2 experienced radiologists blinded to the clinical data, to evaluate hepatobiliary abnormalities. Sensitivity, specificity, and likelihood ratios were calculated. Results: Bile duct irregularities were the most common finding in the IBD-PSC group, with a frequency of 91%. Intra- and extrahepatic bile duct (IHBD and EHBD) irregularities were observed in 87% and 78% of PSC patients, respectively. Higher frequency of IHBD and EHBD wall thickening, bile duct dilation, EHBD stricture, and periportal oedema were observed in the IBD-PSC group. Peribiliary T2-weighted hyperintensities and contrast-enhancement were significantly more common in the IBD-PSC group than in the IBD and HC groups (48% and 35%, respectively) (p < 0.001). Detection of biliary irregularities on MRE had a specificity of 94% (95% CI: 82-99%), a sensitivity of 91% (95% CI: 72-99%), and a positive likelihood ratio of 14.0 (95% CI: 4.7-42.1) for the diagnosis of PSC. Conclusions: This study emphasizes the importance of assessing and reporting hepatobiliary abnormalities visible in the MRE of patients with IBD to avoid a delayed diagnosis of PSC

General Health Subcomponents and Marital Satisfaction: Examining a Correlation during COVID-19

Introduction: COVID-19 is known as a general health threat. General health can play a significant role in marital adjustment and satisfaction and thus the strength of the family foundation. The current study aimed to determine the correlation between families’ general health subcomponents and marital satisfaction during the COVID-19 pandemic in Khaf, Iran. Materials and Methods: This descriptive-analytical study was conducted on 90 married people using the convenience sampling. The study instruments involved a demographics form, a general health questionnaire, and a marital satisfaction questionnaire. Data analyses were performed in SPSS statistical software ver. 22, using the Kolmogorov-Smirnov, Pearson correlation coefficient, multiple regression, independent t-test, one-way analysis of variance, and Tukey’s post hoc tests. The level of significance was set at p &lt;.05. Results: Among the general health subcomponents, the highest mean score belonged to depression (12.80 ± 4.65) and the lowest to social dysfunction (12.04 ± 4.24). The general health score was 49.70 ± 17.35, and the marital satisfaction mean score was 123.13 ± 34.02. Marital satisfaction was negatively and significantly associated with general health (r = -.71) as well as the subcomponents of somatic symptoms (r = -.64), anxiety (r = -.71), social dysfunction (r = -.66), and depression (-.067) (p &lt;.001). Conclusion: As COVID-19 depression and anxiety have the most significant impact on individuals’ general health and marital satisfaction, healthcare managers and policymakers are advised to consider solutions to these disorders in families. As such, they can contribute to spouses’ general health and marital satisfaction and thus strengthen the family foundation

Normal lung tissue complication probability in MR-Linac and conventional radiotherapy

PurposeTo study normal lung tissue (NLT) complications in magnetic resonance (MR) image based linac and conventional radiotherapy (RT) techniques.Materials and MethodsThe Geant4 toolkit was used to simulate a 6 MV photon beam. A homogenous magnetic field of 1.5 Tesla (T) was applied in both perpendicular and parallel directions relative to the radiation beam.Analysis of the NLT complications was assessed according to the normal lung tissue complication probability (NTCP), the mean lung dose (MLD), and percentage of the lung volume receiving doses greater than 20 Gy (V20), using a sample set of CT images generated from a commercially available 4D-XCAT digital phantom.ResultsThe results show that the MLD and V20 were lower for MR-linac RT. The largest reduction of MLD and V20 for MR-linac RT configurations were 5 Gy and 29.3%, respectively.ConclusionMR-linac RT may result in lower NLT complications when compared to conventional RT