AKTIVITAS ANTIOKSIDAN EKSTRAK LAMUN (Enhalus acoroides) ASAL PULAU LAE-LAE MAKASSAR TERHADAP RADIKAL ABTS

Antioxidants are compounds that can inhibit or prevent oxidation reactions, which are the result of chemical reactions and metabolic processes that occur in the body. Enhalus acoroides is a type of seagrass found in Indonesian waters, generally living in sandy sediments with high bioturbation. Lamun has good nutrition and fiber so that it can be a source of food and medicine. The aim of this study was to examine the antioxidant activity of the seagrass extract Enhalus acoroides against ABTS radicals (2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid). The results of the study of seagrass extract using ethanol solvents had a very strong antioxidant category ie 38,008 µg /ml, the smaller the absorbance value, the higher the inhibitory value in which the antioxidant activity of the extract is higher, but when compared with the routine compound control of 0.500 µg /ml, seagrass ethanol extract is still lower

EVALUASI KRIM ANTIOKSIDAN SOLID LIPID MICROPARTICLE EKSTRAK ETANOL DAUN KERSEN (Muntingia calabura)

ABSTRAK Kersen (Muntingia calabura L.) leaves is a plant that contains flavonoids as antioxidants which can be formulated in Solid Lipid Microparticle. The aim of this study is to make Solid Lipid Microparticle cream loaded ethanol extract of kersen leaves preparation and evaluated it’s physical stability. Solid Lipid Microparticle was made of 3% kersen leaves ethanol extract with entrapment efficiency 85.75% formulated in cream preparation with variation of cetyl alcohol concentration namely F1 (2%), F2 (4%), F3 (6%), then tested it’s stability before and after accelerated storage using climatic chamber. The results showed that the three formulas fulfilled the quality of preparation with organoleptic, homogenity, type of cream, viscosity, pH, and spreadability parameters before and after accelerated conditions. Keywords : Kersen leaves, Muntingia calabura L., physical stability, Solid Lipid Microparticle Cream

Diffusion Study of Cream Formula of Lyophilized Ethanol Extract of Moringa Oleifera L. Leaves : Studi Difusi Formula Krim Ekstrak Etanol Terliofilisasi Daun Kelor (Moringa Oleifera L.)

Background: Moringa oleifera L. leaves have analgesic activity due to their flavonoids content. These polar antioxidants compounds usually have difficulty to diffuse through the skin. Objectives: The purpose of this study was to evaluate the diffusion parameters of cream contains the lyophilized of M. oleifera leaves ethanol extract. Material and Methods: The lyophilized of M.oleifera leaves ethanol extract (5%) formulated as an o/w cream with 2% of cetyl alcohol as a stiffening agent. The physical characteristics of the cream were determined for organoleptic, homogeneity, pH, viscosity, spreadability, and type of emulsion parameters. The diffusion parameters were determined with Franz cell diffusion using the skin's snake as a membrane with 0; 10; 30; 60 and 90 minutes sampling time. Results: Physical characteristics of the cream showed that o/w as the cream type with pH, viscosity, and spreadability value were 4.72±0.03; 14.000±225 cps; and 5.7±0.20 cm, respectively. The diffusion parameter showed total flavonoids penetrate through the membrane was 360.134±0.20 µg/cm2 with flux 239,875 µg/cm2hr. Conclusions: This study showed a cream formulation that fulfills the required physical characteristics standard and can deliver active compounds to penetrate through the membrane

In vitro antioxidant and cytotoxic evaluation of ethyl acetate fraction of angiopteris ferox copel tuber against htb lung cancer cell

The tubers of Angiopteris ferox Copel from the Mirrataceae family have been widely used empirically by society for various treatments. This experimentation aimed to discover the antioxidant and anticancer action of the ethyl acetate (EA) fraction of Angiopteris ferox Copel tubers against HTB lung cancer and normal cells in vitro. The EA-fraction obtained from the liquid-liquid extraction was tested for antioxidant activity using the beta carotene degradation and the hydroxyl radical method. The EA-fraction tries the cytotoxic sequel on lung cancer and normal cells using the MTT test method. The outcome achieved that the EA-fraction of the sample has antioxidant activity in reducing lipid peroxidation radicals with an IC50 grade of 30.29 μg /mL (extreme category) and hydroxyl radicals with an IC50 grade of 126.62 μg /mL (medium category). In cytotoxic activity has a medium toxic effect on pulmonary HTB cell line with an IC50 grade of 78.96 μg /mL. In comparison, normal Vero cells have a feeble harmful impact with an IC50 rate >500 μg /mL. It could have culminated that the EA-fraction of Angiopterix ferox Copel has antiradical activity and has a toxic effect on HTB cancer cells but is weak against normal Vero cells. It shall promote as a remedy for the management of lung cancer