Geni indotti da mercurio in Trichoderma harzianum

Il lavoro della presente tesi ha riguardato lo studio della capacit\ue0 di Trichoderma harzianum di crescere in presenza di mercurio. L\u2019obiettivo principale era quello di comprendere i meccanismi biomolecolari coinvolti nella risposta metabolica allo stress indotto da mercurio in T. harzianum. E\u2019 stato studiato l\u2019accrescimento radiale su piastra in terreno colturale agarizzato in presenza di cadmio e mercurio e il peso secco del micelio del fungo accresciutosi in terreno colturale liquido nelle medesime condizioni. In entrambi i casi T. harzianum ha mostrato una maggiore capacit\ue0 di crescita in presenza di mercurio. In un secondo tempo, la ricerca ha avuto come obiettivo quello di identificare i geni differenzialmente espressi nel fungo cresciuto in presenza dei gi\ue0 citati metalli pesanti. La costruzione di due SSH library, una specifica per il mercurio e una per il cadmio, ha permesso d\u2019isolare e identificare i geni differenzialmente espressi nel micelio del fungo cresciuto in presenza di cadmio e in presenza di mercurio. In seguito, focalizzando l\u2019attenzione sui geni attivati solo in presenza di mercurio, applicando la tecnica della Real-Time RT-PCR, sono stati studiati i livelli d\u2019espressione di questi geni sia in T. harzianum cresciuto in presenza di mercurio e in presenza di cadmio, sia in assenza dei suddetti metalli pesanti (controllo). I risultati hanno mostrato una sostanziale sovraespressione di questi geni nel micelio di T. harzianum cresciuto in presenza di mercurio rispetto allo stesso cresciuto in presenza di cadmio e nel controllo. La sovraespressione dei geni codificanti per proteine della parete cellulare, suggerirebbe un ruolo attivo e fondamentale di queste nei meccanismi di resistenza al mercurio messi in atto dal fungo

A One-Pot Synthesis of "K(hfa) glyme" Adducts: Effect of the Polyether Length on the Ion Coordination Sphere

AbstractPotassium complexes are starting to gather more and more interest from academia and industry because of their intriguing application possibilities. Novel adducts of potassium hexafluoroacetylacetonato [K(hfa)] with polyethers (monoglyme, diglyme, triglyme, and tetraglyme) were synthesized through a single step reaction and characterized through FT‐IR spectroscopy as well as 1H and 13C NMR spectroscopy. Single crystal X‐ray diffraction studies enabled the identification of fascinating K coordination polymeric networks

The Immanent Body: A Thematic Study of the Logic of Immanence in Christology, Philosophy and Aesthetics

This dissertation is comprised of two sections: 1) The Immanent Body in Late Antiquity : an analysis of late ancient christology, Stoicism and philosophy; 2) The Extended Body: Aesthetics : depictions of monastic bodies in late medieval, Renaissance and contemporary art. I. The thesis of this dissertation can be stated as follows: the conditions under which immanence is thinkable in relation to bodies are found in conceptual personae. Contemporary philosopher Gilles Deleuze\u27s concept of conceptual personae, developed in conjunction with French theorist Felix Guattari, helps navigate the complex relationship between bodies and ontology developed by these three ancient thinkers. In order to understand the formation of the conceptual persona of Christ in late antiquity, it was necessary to return to the work of Irenaeus, Tertullian, and Athanasius. II. The second part of the project begins with the Antony Series, which is a cluster of Renaissance works of art designated by a common theme. They represent St. Antony, who, being the first deep desert monastic, was the subject of my previous chapters. Therefore, the link between late antique conceptions of the monastic body and Renaissance art becomes explicit. Early Renaissance artists turned to an aesthetics of the monastic body in order to revolutionize painting, for it was during the late thirteenth century that expressive bodies were being created, bodies that would move painting towards the Renaissance. This dissertation analyzes the precise point of this transition through a christological (i.e., monastic) understanding of the painted figure

InGaAs/InP SPAD with Monolithically Integrated Zinc-Diffused Resistor

Afterpulsing and optical crosstalk are significant performance limitations for applications employing near-infrared single-photon avalanche diodes (SPADs). In this paper, we describe an InGaAs/InP SPAD with monolithically integrated resistor that is fully compatible with the planar fabrication process and provides a significant reduction of the avalanche charge and, thus, of afterpulsing and optical crosstalk. In order to have a fast SPAD reset (<50 ns), we fabricated quenching resistors ranging from 10 to 200 k\Ω, smaller than what is available in the literature. The resistor, fabricated with the zinc diffusions already used for avoiding premature edge-breakdown, promptly reduces the avalanche current to a low value ∼ 100~ μ A in less than 1 ns, while an active circuit completes the quenching and enforces a well-defined hold-off. The proposed mixed-quenching approach guarantees an avalanche charge reduction of more than 20 times compared with similar plain SPADs, enough to reduce the hold-off time down to 1 μ s. Finally, a compact single-photon counting module based on this detector and featuring 70-ps photon-timing jitter is presented

Optical Crosstalk in InGaAs/InP SPAD Array: Analysis and Reduction with FIB-Etched Trenches

This letter describes the reduction of optical crosstalk by means of focused ion beam-etched trenches in InGaAs/InP single-photon avalanche diode arrays. Platinum-filled trenches have been fabricated in a linear array in order to limit the direct optical crosstalk between neighboring pixels. Experimental measurements prove that optical crosstalk has been reduced by ∼60 % thanks to a strong suppression of direct optical paths. An optical model is introduced in order to describe the main contributions to crosstalk and to validate measurements

Single-Photon Avalanche Diodes in a 0.16 μm BCD Technology With Sharp Timing Response and Red-Enhanced Sensitivity

CMOS single-photon avalanche diodes (SPADs) have recently become an emerging imaging technology for applications requiring high sensitivity and high frame-rate in the visible and near-infrared range. However, a higher photon detection efficiency (PDE), particularly in the 700-950 nm range, is highly desirable for many growing markets, such as eye-safe three-dimensional imaging (LIDAR). In this paper, we report the design and characterization of SPADs fabricated in a 0.16 mu m BCD (Bipolar-CMOS-DMOS) technology. The overall detection performance is among the best reported in the literature: 1) PDE of 60% at 500 nm wavelength and still 12% at 800 nm; 2) very low dark count rate of < 0.2 cps/mu m(2) (in counts per second per unit area); 3) < 1% afterpulsing probability with 50 ns dead-time; and 4) temporal response with 30 ps full width at half-maximum and less than 50 ps diffusion tail time constant

Perspectives for the small historical centres at risk of abandonment. A pilot project for the Granfonte district in Leonforte (Italy)

[EN] The town of Leonforte, in Sicily, is currently characterised by two antithetical phenomena: the abandoment of a significant part of the historic centre and an increasing urban sprawl in the peripheral areas. The paper illustrates the ongoing research on the historic district of Granfonte and the pilot project for a small block with two alternative scenarios: the restoration of the ruins and the recovery of residential use. The two proposals can be conceived as two phases of the same project, and as alternatives to the demolitions that nowadays seem to be the only answer to the daily challenge of living in this place.Vitale, M.; Circo, C.; Sanzaro, D.; Sebàstian Franco, S.; Cacciatore, I.; Massimino, M. (2022). Perspectives for the small historical centres at risk of abandonment. A pilot project for the Granfonte district in Leonforte (Italy). Editorial Universitat Politècnica de València. 937-944. https://doi.org/10.4995/HERITAGE2022.2022.1452893794