Stelleninhaber geht – Wissen bleibt!

In Deutschland nimmt der Anteil älterer Arbeitnehmerinnen und Arbeitnehmer tendenziell zu. Deshalb muss sich die Bibliotheksleitung verstärkt auf das altersbedingte Ausscheiden älterer Arbeitnehmer einstellen. Eine langjährige Fachkraft verfügt über spezielles Erfahrungswissen im direkten Aufgabenfeld. Die Bibliotheksleitung muss den Transfer allen relevanten Wissens, dazu gehört das Erfahrungswissen, vom Stelleninhaber auf seinen Nachfolger ermöglichen und unterstützen. Am Beispiel der Universitätsbibliothek der Bergakademie Freiberg wird untersucht, wie das Wissensmanagement im Rahmen eines Stellenwechsels derzeit geregelt ist. Das geschieht mit Hilfe von Tiefeninterviews in verschiedenen Abteilungen. Die Auswertung der Interviews bildet die Basis für ein Konzept für das Wissensmanagement beim Stellenwechsel an der UB Freiberg. Das Konzept benennt u. a. Maßnahmen zur Identifikation des stellenbezogenen Wissens, Maßnahmen zur Dokumentation des relevanten Wissens und Instrumente zur Wissensweitergabe beim Stellenwechsel

Bioenergy Technologies for a Net Zero Transition:Outcomes of UK-India Bioenergy Research Scoping

The report is part of scoping exercise led by UK Research and Innovation (UKRI)’s Engineering and Physical Sciences Research Council (EPSRC) and Biotechnology and Biological Sciences Research Council (BBSRC) and commissioned to Supergen Bioenergy Hub. The report is for UKRI, funded by UKRI India. UKRI launched in April 2018. UKRI is a non-departmental public body sponsored by the Department for Business, Energy and Industrial Strategy (BEIS). Our organisation brings together the seven disciplinary research councils, Research England, which is responsible for supporting research and knowledge exchange at higher education institutions in England, and the UK’s innovation agency, Innovate UK. Our nine councils work together in innovative ways to deliver an ambitious agenda, drawing on our great depth and breadth of expertise and the enormous diversity of our portfolio. http://www.ukri.org UKRI India plays a key role in enhancing the research and innovation collaboration between the UK and India. Since 2008, the UK and Indian governments, and third parties, have together invested over £330 million in co-funded research and innovation programmes. This investment has brought about more than 258 individual projects. The projects were funded by over 15 funding agencies, bringing together more than 220 lead institutions from the UK and India. These research projects have generated more than £450 million in further funding, mainly from public bodies but also from non-profit organisations and commercial entities, attesting the relevance of these projects. www.ukri.org/india This work was commissioned to inform UKRI/UKRI India priorities and pathways for innovation development in bioenergy with UK-India partnerships

Tissue Transglutaminase Constitutively Activates HIF-1α Promoter and Nuclear Factor-κB via a Non-Canonical Pathway

<div><p>Constitutive activation of nuclear factor kappa B (NF-κB) has been linked with carcinogenesis and cancer progression, including metastasis, chemoresistance, and radiation resistance. However, the molecular mechanisms that result in constitutive activation of NF-κB are poorly understood. Here we show that chronic expression of the pro-inflammatory protein tissue transglutaminase (TG2) reprograms the transcription regulatory network in epithelial cells via constitutive activation of NF-κB. TG2-induced NF-κB binds the functional NF-κB binding site in hypoxia-inducible factor-1 (<em>HIF-1α</em>) promoter and results in its increased expression at transcription and protein levels even under normoxic conditions. TG2/NF-κB-induced HIF-1 was deemed essential for increased expression of some transcription repressors, like <em>Zeb1</em>, Zeb2, <em>Snail</em>, and <em>Twist</em>. Unlike tumor necrosis factor-alpha (TNFα), TG2 did not require IκB kinase (IKK) for NF-κB activation. Our data suggest that TG2 binds with IκBα and results in its rapid degradation via a non-proteasomal pathway. Importantly, the catalytically inactive (C277S) mutant form of TG2 was as effective as was wild-type TG2 in activating NF-κB and inducing HIF-1 expression. We also found that TG2 interacted with p65/RelA protein, both in the cytosolic and the nuclear compartment. The TG2/p65(NF-κB) complex binds to the <em>HIF-1</em> promoter and induced its transcriptional regulation. Inhibition of TG2 or p65/RelA also inhibited the HIF-1α expression and attenuated <em>Zeb1, Zeb2</em>, and <em>Twist</em> expression. To our knowledge, these findings show for the first time a direct link between TG2, NF-κB, and HIF-1α, demonstrating TG2's important role in cancer progression.</p> </div

The sequence of (a)TAR RNA and (b) Tat peptide along with different lysine modifications and their positions.

<p>The sequence of (a)TAR RNA and (b) Tat peptide along with different lysine modifications and their positions.</p

ITC titration profile of TAR RNA with Tat and different modified peptides at 25 °C.

<p>Upper panel shows the baseline corrected experimental data for peptide binding, lower panel shows the molar heats of binding (□) plotted against the peptide to RNA molar ratio. Buffer condition was as described in the caption to <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0077595#pone-0077595-g002" target="_blank">Figure 2</a>. Molar heat of binding is calculated by integration of the area under the curve of each heat burst using the origin version 7.0 software (Microcal, Inc.; Northampton, MA). Fitting of ITC data (shown as solid line) was done using model for two set of binding [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0077595#B38" target="_blank">38</a>] given in origin version 7.0 software (Microcal, Inc.; Northampton, MA).</p

CD spectra TAR RNA and the effect of binding of different peptides on the structure of the RNA at 25 °C.

<p>(□) 2 μM RNA, (○) 10 μM Peptide. All CD spectra were collected in a buffer containing 10 mM sodium cacodylate, 70 mM NaCl and 0.l mM EDTA at pH 7.5. </p

Market, Freedom and the Illusions of Microcredit. Patronage, Caste, Class and Patriarchy in Rural South India

<p>As a market tool, microcredit is expected to promote individual freedom, for women in particular. By drawing on a southern Indian case, this paper argues that microcredit is in fact shaped by the power structures it is supposed to eradicate. Even if they are partly reshaped, local structures of power remain unavoidable to protect populations (something that microcredit fails to do) but also to build the microcredit market and ensure its legitimacy, for donors, local political arenas and local populations. Far beyond microcredit, our findings question the uneasy relationships between markets and individual freedoms.</p

Schematic representation of nuclear run-on assay and mechanism of incorporation of radiolabelled ³²P-UTP and visualization of relative rate of transcription (Partially adapted from Weber et al., 1977).

<p>(a) <i>in-vitro</i> transcription (b) paused polymerase with cognate templates (c) reactivation of polymerase and incorporation of labeled UTPs (d) measurement of relative transcription rate.</p

Geochemistry of biotites and host granitoid plutons from the Proterozoic Mahakoshal Belt, central India tectonic zone: implication for nature and tectonic setting of magmatism

<div><p>The northern part of the central India tectonic zone (CITZ) is occupied by the Proterozoic Mahakoshal Belt, which is mainly comprised of granitoids and volcano-sedimentary lithounits. The granitoids (ca. 1880–1710 Ma) are exposed as small circular to elliptical-shaped, stock-like intrusive bodies, such as Nerueadamar granitoids (NG), Tumiya granitoids (TG), Jhirgadandi granitoids (JG), Dudhi granite gneiss (DG), Raspahari granitoids (RG), Katoli granitoids (KG), and Harnakachar granitoids (HG), collectively forming the granite gneissic complex (GGC). The geochemistry of biotites, host granitoids, and enclaves from these plutons has been investigated in order to understand the redox condition and likely tectonic affinity of host granitoids. The Al₂O₃–MgO–FeO^t contents and operated elemental substitution in biotites strongly suggest the diverse nature of host magmas such as calc-alkaline, metaluminous (I-type), peraluminous (S-type), and transitional between I- and S-types, which appear to have formed in subduction zone and syn-collisional tectonic settings. The transitional (I-S)-type granitoids inferred based on biotite compositions, however, represent both metaluminous (HG) and peraluminous (DG and KG) granitoids in terms of whole-rock molar A/CNK (Al₂O₃/CaO + Na₂O + K₂O) ratios. Ages of granitoid magmatism and its field association with contemporaneous volcano-sedimentary lithounits clearly mark the post-collisional tectonic setting, which contradicts the subduction-related tectonic setting inferred from biotites of JG and microgranular enclave (JE) hosted in JG. Whole-rock major and trace elements broadly suggest the existence of collision tectonics during the formation of granitoid plutons. The JG, KG, and DG contain a bt-Kf-mag-qtz assemblage, and their parental magmas evolved under moderate oxidizing environments (ƒO₂ = −12.03 to −13.27 bars). On the other hand, RG (bt-gt-Kf-pl-qtz), NG (bt-ms-Kf-pl-qtz), and TG (bt-ms-Kf-pl-qtz) represent pure crustal-derived magmas evolved in strongly reducing conditions formed under a syn-collisional tectonic setting as evident from their mineral assemblages and biotite and whole-rock compositions. Granitoid plutons of the Mahakoshal Belt were most likely formed during amalgamation of the Columbian supercontinent.</p></div

Overview of isolation of transcriptionally active nuclei from <i>C</i>. <i>roseus</i> accessions (a) pre-spin gradient (b) post-spin gradient (c) nuclei layer (d) DAPI stained nuclei (e) Differential interference contrast (DIC) view of nuclei.

<p>Overview of isolation of transcriptionally active nuclei from <i>C</i>. <i>roseus</i> accessions (a) pre-spin gradient (b) post-spin gradient (c) nuclei layer (d) DAPI stained nuclei (e) Differential interference contrast (DIC) view of nuclei.</p