1,654 research outputs found

    Diversity, floristic composition, and structure of the woody vegetation of the Cerrado in the Cerrado–Amazon transition zone in Mato Grosso, Brazil

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    The final publication is available at Springer via http://dx.doi.org/10.1007/s40415-015-0186-2We compared the diversity and species composition and the structure of the vegetation of three distinct Cerrado phytophysiognomies (Cerradão, Dense Cerrado, and Typical Cerrado) in the Cerrado–Amazon transition, Mato Grosso (Brazil). Species richness (observed and estimated) in the Cerradão and Dense Cerrado was higher than that recorded in the Typical Cerrado. Species diversity, based on a Rényi profile, was highest in the Dense Cerrado, in comparison with the other phytophysiognomies. We recorded a higher number of exclusive species in the Cerradão and a greater similarity (Morisita and Sørensen indices) between this vegetation type and the Dense Cerrado. While individuals were tallest in the Cerradão and Dense Cerrado and lowest in the Typical Cerrado, there was no difference among phytophysiognomies in mean diameter. A gradient in decreasing species richness and diversity (hypothesis 1) and vegetation vertical structure (hypothesis 3) was expected for the Cerradão–Dense Cerrado–Typical Cerrado; however, neither hypothesis was supported by the results. The Cerradão and Dense Cerrado were most similar in species composition not confirming hypothesis 2, which predicted that the two savanna vegetation types (Dense Cerrado and Typical Cerrado) were more similar to one another than either is to the woodland (Cerradão). Overall, the similarities among the three study communities depended on the type of parameter analyzed. While the species richness and the vertical and structure of the vegetation of the Cerradão and Dense Cerrado are closely similar, the Cerradão and Typical Cerrado are more similar in their species diversity. With regard to the floristic composition, Dense Cerrado occupies an intermediate position between Cerradão and Typical Cerrado.UNEMAT Graduate Program in Ecology and ConservationBrazilian Higher Education Training Program (CAPES)“Tropical Biomes in Transition – TROBIT”CAPES/Science without Borders ProgramPELD/CNPq (Long-Term Ecological Studies)PROCAD UnB/UNEMA

    A Molecular Analysis of Cytokine Content across Extracellular Vesicles, Secretions, and Intracellular Space from Different Site-Specific Adipose-Derived Stem Cells

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    Cytokines are multifunctional small proteins that have a vital influence on inflammatory states of tissues and play a role in signalling and cellular control mechanisms. Cytokine expression has primarily been viewed as a form of direct secretion of molecules through an active transportation; however, other forms of active transport such as extracellular vesicles are at play. This is particularly important in stem cells where signalling molecules are key to communication managing the levels of proliferation, migration, and differentiation into mature cells. This study investigated cytokines from intracellular content, direct cellular secretions, and extracellular vesicles from adult adipose-derived stem cells isolated from three distinct anatomical locations: abdomen, thigh, and chin. The cells were cultured investigated using live cell microscopy, cytokine assays, and bioinformatics analysis. The cytokines quantified and examined from each sample type showed a distinct difference between niche areas and sample types. The varying levels of TNF-alpha, IL-6 and IL-8 cytokines were shown to play a crucial role in signalling pathways such as MAPK, ERK1/2 and JAK-STAT in cells. On the other hand, the chemotactic cytokines IL-1rn, Eotaxin, IP-10 and MCP-1 showed the most prominent changes across extracellular vesicles with roles in noncanonical signalling. By examining the local and tangential roles of cytokines in stem cells, their roles in signalling and in regenerative mechanisms may be further understood.</jats:p

    Study of structural changes in amorphous germanium-nitrogen alloys by optical techniques

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    Thin films of amorphous germanium-nitrogen (alpha-GeN) alloys prepared by the rf sputtering deposition technique have been studied by Raman scattering spectroscopy. The nitrogen content of the samples varies between zero and approximate to 3 x 10(22) atoms cm(-3), as determined from nuclear reaction analysis data. Modifications of the structural characteristics of the Ge-N alloys, probed through their phonon density, were investigated as a function of the nitrogen concentration. In addition to an increase of the network's topological disorder, nitrogen is responsible, at relatively high concentrations, for a structural transition in the alpha-Ge host. The optical and electronic characterization of the samples confirm these changes which are highly dependent on the nitrogen concentration. (C) 1996 American Institute of Physics.79143343

    STRUCTURE OF INTERFACES IN A-SI-H/A-SINX-H SUPERLATTICES

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    We present experimental results on the atomic structure of the interfaces between a-Si: H and a-SiN(x):H layers obtained by analyzing the intensity of the Raman lines from zone-folded acoustic phonons and of the peaks of x-ray diffraction at grazing angles. We determine the width of these interfaces and their stability under thermal annealing in temperatures below the crystallization temperature.69277878

    Laser crystallization and structuring of amorphous germanium

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    The short-pulse laser crystallization and interference structuring of amorphous germanium films were investigated by time resolved reflection measurements and Raman spectroscopy, We demonstrate that submicrometer crystalline structures with very sharp lateral interfaces can be produced by laser interference crystallization of nonhydrogenated samples. In hydrogenated films, on the other hand, the film surface disrupts upon laser exposure leading to the formation of a free-standing crystalline membrane, The Raman spectra of laser crystallized germanium display effects of finite crystallite size and stress. (C) 1997 American Institute of Physics.70263570357

    Epitaxial pulsed laser crystallization of amorphous germanium on GaAs

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    We have investigated the crystallization of amorphous germanium films on GaAs crystals using nanosecond laser pulses. The structure and composition of the crystallized layers is dominated by nonequilibrium effects induced by the fast cooling process following laser irradiation. Perfect epitaxial films are obtained for fluencies that completely melt the Ge film, but not the substrate. For higher fluencies, partial melting of the substrate leads to the formation of a (GaAs)(1-x)Ge-2x epitaxial alloy with a graded composition profile at the interface with the substrate. Since Ge and GaAs are thermodynamically immiscible in the solid phase, the formation of the alloy is attributed to the suppression of phase separation during the fast cooling process. Lower laser fluencies lead to polycrystalline layers with a patterned surface structure. The latter is attributed to the freeze-in of instabilities in the melt during the fast solidification process. (C) 2001 American Institute of Physics.9052575258

    Serum biomarkers associated with SARS-CoV-2 severity

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    Immunity with SARS-CoV-2 infection during the acute phase is not sufficiently well understood to differentiate mild from severe cases and identify prognostic markers. We evaluated the immune response profile using a total of 71 biomarkers in sera from patients with SARS-CoV-2 infection, confirmed by RT-PCR and controls. We correlated biological marker levels with negative control (C) asymptomatic (A), nonhospitalized (mild cases-M), and hospitalized (severe cases-S) groups. Among angiogenesis markers, we identified biomarkers that were more frequently elevated in severe cases when compared to the other groups (C, A, and M). Among cardiovascular diseases, there were biomarkers with differences between the groups, with D-dimer, GDF-15, and sICAM-1 higher in the S group. The levels of the biomarkers Myoglobin and P-Selectin were lower among patients in group M compared to those in groups S and A. Important differences in cytokines and chemokines according to the clinical course were identified. Severe cases presented altered levels when compared to group C. This study helps to characterize biological markers related to angiogenesis, growth factors, heart disease, and cytokine/chemokine production in individuals infected with SARS-CoV-2, offering prognostic signatures and a basis for understanding the biological factors in disease severity
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