Evidence that the degree of band 3 phosphorylation modulates human erythrocytes nitric oxide efflux – in vitro model of hyperfibrinogenemia

© 2011 – IOS Press and the authors. All rights reservedRecent evidence has shown that plasma fibrinogen, a major cardiovascular risk factor, interacts with the erythrocyte membrane and acts to influence blood flow via erythrocyte nitric oxide (NO) modulation. In the present pioneer in-vitro study, whole blood samples were harvested from healthy subjects and aliquots were incubated in the absence (control aliquots) and presence of fibrinogen at different degrees of band 3 phosphorylation, and the levels of NO, nitrite, nitrate and S-nitroglutathione (GSNO) were determined. Hyperfibrinogenemia interferes with erythrocyte NO mobilization without changing its efflux in a way that seems to be dependent of the degree of band 3 phosphorylation. In presence of higher fibrinogen concentrations the NO efflux is reinforced when band 3 is phosphorylated (p < 0.001). Higher levels of nitrite, nitrate and GSNO were documented (p < 0.05). However, the mechanisms by which fibrinogen signalling modulates erythrocyte function remain to be clarified and are currently under study. These conditions may be considered an approach to be followed in blood storage for transfusions.This study was supported by grants from the FCT - Fundação para a Ciência e a Tecnologia (project reference PTDC/SAU-OSM/73449/2006

Observation of dipole-dipole interaction in a degenerate quantum gas

We have investigated the expansion of a Bose-Einstein condensate (BEC) of strongly magnetic chromium atoms. The long-range and anisotropic magnetic dipole-dipole interaction leads to an anisotropic deformation of the expanding Cr-BEC which depends on the orientation of the atomic dipole moments. Our measurements are consistent with the theory of dipolar quantum gases and show that a Cr-BEC is an excellent model system to study dipolar interactions in such gases.Comment: 4 pages, 2 figure

Knowledge and attitude towards the gradual reduction of salt in bread – an online survey

Aim: Assess knowledge and attitude towards the gradual reduction of salt in bread and the potential impact on eating habits of children (6-18 years) and their families, as part as a Health Impact Assessment pilot study.N/

A thorough analysis of the short- and mid-term activity-related variations in the solar acoustic frequencies

The frequencies of the solar acoustic oscillations vary over the activity cycle. The variations in other activity proxies are found to be well correlated with the variations in the acoustic frequencies. However, each proxy has a slightly different time behaviour. Our goal is to characterize the differences between the time behaviour of the frequency shifts and of two other activity proxies, namely, the area covered by sunspots and the 10.7cm flux. We define a new observable that is particularly sensitive to the short-term frequency variations. We then compare the observable when computed from model frequency shifts and from observed frequency shifts obtained with the Global Oscillation Network Group (GONG) for cycle 23. Our analysis shows that on the shortest time-scales the variations in the frequency shifts seen in the GONG observations are strongly correlated with the variations in the area covered by sunspots. However, a significant loss of correlation is still found. We verify that the times when the frequency shifts and the sunspot area do not vary in a similar way tend to coincide with the times of the maxima of the quasi-biennial variations seen in the solar seismic data. A similar analysis of the relation between the 10.7cm flux and the frequency shifts reveals that the short-time variations in the frequency shifts follow even more closely those of the 10.7cm flux than those of the sunspot area. However, a loss of correlation between frequency shifts and 10.7cm flux variations is still found around the same times.Comment: 7 pages, 6 figures, accepted for publication in MNRA