Anti-inflammatory, antinociceptive, and antipyretic effects of methanol extract of Cariniana rubra stem bark in animal models

Schmeda-Hirschmann, G (Schmeda-Hirschmann, Guillermo) Univ Talca, IQRN, Talca, ChileCariniana rubra Miers (Lecythidaceae), popularly known as "jequitiba-vermelho", is a large Brazilian tree whose bark is used in infusion and decoction for the treatment of inflammatory conditions. This study aims to assess the anti-inflammatory, antinociceptive, and antipyretic effects of Cariniana rubra methanolic stem bark extract (EMCr) using experimental animals. Anti-inflammatory activity of EMCr was tested on carrageenan and dextran-induced rat paw edema, carrageenan-induced pleurisy in rats and acetic acid-increase vascular permeability in mice. Antinociceptive and antipyretic activities were evaluated using acetic acid-induced writhing, formalin and hot-plate tests in mice, as well as brewer's yeast-induced pyrexia in rats. The extract inhibitied carrageenan and dextran-induced edema, reduced exudate volume and leukocyte migration on the carrageenan-induced pleurisy and on the vascular permeability increase induced by acetic acid. The EMCr inhibited nociception on the acetic acid-induced writhing and in the second phase of formalin test, and decreased rectal temperature. It was, however, inactive against thermal nociception. Phytochemical analysis with EMCr showed the occurrence of saponins, triterpenes, sterols and phenolic compounds. Phytosterols (beta-sitosterol, stigmasterol), pentacyclic triterpenes (alpha- and beta-amyrin as a mixture), arjunolic acid, a phytosterol glycoside (sitosterol 3-O-beta-D-glucopyranoside), and triterpenoid saponins (28-beta-glucopyranosyl-23-O-acetyl arjunolic acid; 3-O-beta-glucopyranosyl arjunolic acid and 28-O-[alpha-L-Rhamnopyranosyl-(1 -> 2)-beta-glucopyranosyl]-23-O-acetyl arjunolic acid) were the main identified compounds. It can be presumed that EMCr caused their effects by inhibiting the liberation and/or action of different inflammatory mediators. These findings support the traditional use of Cariniana rubra preparations to treat inflammation

Collapsing Spheres Satisfying An "Euclidean Condition"

We study the general properties of fluid spheres satisfying the heuristic assumption that their areas and proper radius are equal (the Euclidean condition). Dissipative and non-dissipative models are considered. In the latter case, all models are necessarily geodesic and a subclass of the Lemaitre-Tolman-Bondi solution is obtained. In the dissipative case solutions are non-geodesic and are characterized by the fact that all non-gravitational forces acting on any fluid element produces a radial three-acceleration independent on its inertial mass.Comment: 1o pages, Latex. Title changed and text shortened to fit the version to appear in Gen.Rel.Grav

Stationary Cylindrical Anisotropic Fluid

We present the whole set of equations with regularity and matching conditions required for the description of physically meaningful stationary cylindrically symmmetric distributions of matter, smoothly matched to Lewis vacuum spacetime. A specific example is given. The electric and magnetic parts of the Weyl tensor are calculated, and it is shown that purely electric solutions are necessarily static. Then, it is shown that no conformally flat stationary cylindrical fluid exits, satisfying regularity and matching conditions.Comment: 17 pages Latex. To appear in Gen.Rel.Gra

Energetics of the Einstein-Rosen spacetime

A study covering some aspects of the Einstein--Rosen metric is presented. The electric and magnetic parts of the Weyl tensor are calculated. It is shown that there are no purely magnetic E--R spacetimes, and also that a purely electric E--R spacetime is necessarily static. The geodesics equations are found and circular ones are analyzed in detail. The super--Poynting and the ``Lagrangian'' Poynting vectors are calculated and their expressions are found for two specific examples. It is shown that for a pulse--type solution, both expressions describe an inward radially directed flow of energy, far behind the wave front. The physical significance of such an effect is discussed.Comment: 19 pages Latex.References added and updated.To appear in Int.J.Theor.Phy

Spherically symmetric dissipative anisotropic fluids: A general study

The full set of equations governing the evolution of self--gravitating spherically symmetric dissipative fluids with anisotropic stresses is deployed and used to carry out a general study on the behaviour of such systems, in the context of general relativity. Emphasis is given to the link between the Weyl tensor, the shear tensor, the anisotropy of the pressure and the density inhomogeneity. In particular we provide the general, necessary and sufficient, condition for the vanishing of the spatial gradients of energy density, which in turn suggests a possible definition of a gravitational arrow of time. Some solutions are also exhibited to illustrate the discussion.Comment: 28 pages Latex. To appear in Phys.Rev.

A novel form of deleted in breast cancer 1 (DBC1) lacking the N-terminal domain does not bind SIRT1 and is dynamically regulated in vivo

The protein Deleted in Breast Cancer-1 is a regulator of several transcription factors and epigenetic regulators, including HDAC3, Rev-erb-alpha, PARP1 and SIRT1. It is well known that DBC1 regulates its targets, including SIRT1, by protein-protein interaction. However, little is known about how DBC1 biological activity is regulated. In this work, we show that in quiescent cells DBC1 is proteolytically cleaved, producing a protein (DN-DBC1) that misses the S1-like domain and no longer binds to SIRT1. DN-DBC1 is also found in vivo in mouse and human tissues. Interestingly, DN-DBC1 is cleared once quiescent cells re-enter to the cell cycle. Using a model of liver regeneration after partial hepatectomy, we found that DN-DBC1 is down-regulated in vivo during regeneration. In fact, WT mice show a decrease in SIRT1 activity during liver regeneration, coincidentally with DN-DBC1 downregulation and the appearance of full length DBC1. This effect on SIRT1 activity was not observed in DBC1 KO mice. Finally, we found that DBC1 KO mice have altered cell cycle progression and liver regeneration after partial hepatectomy, suggesting that DBC1/DN-DBC1 transitions play a role in normal cell cycle progression in vivo after cells leave quiescence. We propose that quiescent cells express DN-DBC1, which either replaces or coexist with the full-length protein, and that restoring of DBC1 is required for normal cell cycle progression in vitro and in vivo. Our results describe for the first time in vivo a naturally occurring form of DBC1, which does not bind SIRT1 and is dynamically regulated, thus contributing to redefine the knowledge about its function

Theory and Applications of Non-Relativistic and Relativistic Turbulent Reconnection

Realistic astrophysical environments are turbulent due to the extremely high Reynolds numbers. Therefore, the theories of reconnection intended for describing astrophysical reconnection should not ignore the effects of turbulence on magnetic reconnection. Turbulence is known to change the nature of many physical processes dramatically and in this review we claim that magnetic reconnection is not an exception. We stress that not only astrophysical turbulence is ubiquitous, but also magnetic reconnection itself induces turbulence. Thus turbulence must be accounted for in any realistic astrophysical reconnection setup. We argue that due to the similarities of MHD turbulence in relativistic and non-relativistic cases the theory of magnetic reconnection developed for the non-relativistic case can be extended to the relativistic case and we provide numerical simulations that support this conjecture. We also provide quantitative comparisons of the theoretical predictions and results of numerical experiments, including the situations when turbulent reconnection is self-driven, i.e. the turbulence in the system is generated by the reconnection process itself. We show how turbulent reconnection entails the violation of magnetic flux freezing, the conclusion that has really far reaching consequences for many realistically turbulent astrophysical environments. In addition, we consider observational testing of turbulent reconnection as well as numerous implications of the theory. The former includes the Sun and solar wind reconnection, while the latter include the process of reconnection diffusion induced by turbulent reconnection, the acceleration of energetic particles, bursts of turbulent reconnection related to black hole sources as well as gamma ray bursts. Finally, we explain why turbulent reconnection cannot be explained by turbulent resistivity or derived through the mean field approach.Comment: 66 pages, 24 figures, a chapter of the book "Magnetic Reconnection - Concepts and Applications", editors W. Gonzalez, E. N. Parke