PHYTOCHEMICALS AND HYPOGLYCEMIC PROPERTIES OF METHANOL LEAF EXTRACT OF PHYLLANTHUS AMARUS

Plants and plant products are continuously being explored in medicine against diabetics. In the present study, phytochemical screening and hypoglycemic properties of methanol leaf extract of Phyllanthus amarus against alloxan-induced diabetic rats were conducted. A total of fifteen (15) albino rats were randomly selected into 5 groups of 3 rats per group. Groups 1-3 rats were treated with   250 and 500 mg/kg methanol extract of Phyllanthus amarus and 5 mg/kg of glibenclamide respectively, while groups 4 and 5 served as diabetic and normal controls respectively. All treatments were administered orally, once daily for fifteen days. Results revealed the presence of alkaloids, terpenoids, saponins, glycosides, flavonoids, total phenols and tannins. A significant (P<0.05) and a progressive increase in blood glucose level were observed in diabetic untreated rats throughout the experimental periods. At the end of the treatment period rat treated with 250 & 500 mg/kg, P. amarus had final glucose level of 117.00±5.43 mg/dl & 106.00±4.56 mg/kg. Rats treated with 2.5mg/kg glibenclamide had final glucose level of 117.33±5.43 while the untreated rat had final glucose level of 562.50±15.45 mg/dl. The extract of phyllanthus amarus at 250 and 500 mg/kg also cause a significant improvement in body weight gain of the rat. It is concluded that phyllanthus amarus contains some useful phytochemicals with potential hypoglycemic reputations. Thus, it may be considered as a natural source of the drug for therapeutic purposes

Greenness assessment of chromatographic methods used for analysis of empagliflozin: a comparative study

The analytical chemistry community is attempting to incorporate green chemistry concepts in the development of analytical techniques to redefine analytical methods and dramatically modify the philosophy of analytical technique development. Each greenness assessment method has its own benefits and drawbacks, as well as its own procedures. The results of each greenness assessment method produce numerous deductions regarding the selection of a greenest chromatographic method on which the determination of a greenness assessment tool depends. The current study examined the greenness behavior of 26 reported chromatographic methods in the literature for the evaluation of the medicine empagliflozin using three evaluation methods: the national environmental methods index (NEMI), the eco-scale assessment (ESA), and the green analytical procedure index (GAPI). This comparative study discussed the value of using more than one greenness evaluation methods while evaluating. The findings showed that the NEMI was a less informative and misleading tool. However, the ESA provided reliable numerical assessments out of 100. Despite the GAPI being a complex assessment compared to the others, it provided a fully descriptive three-colored pictogram and a precise assessment. The findings recommended applying more than one greenness assessment tool to evaluate the greenness of methods prior to planning laboratory-based analytical methods to ensure an environment friendly process

Detection and quantification of new psychoactive substances (NPSs) within the evolved "legal high" product, NRG-2, using high performance liquid chromatography-amperometric detection (HPLC-AD)

The global increase in the production and abuse of cathinone-derived New Psychoactive Substances (NPSs) has developed the requirement for rapid, selective and sensitive protocols for their separation and detection. Electrochemical sensing of these compounds has been demonstrated to be an effective method for the in-field detection of these substances, either in their pure form or in the presence of common adulterants, however, the technique is limited in its ability to discriminate between structurally related cathinone-derivatives (for example: (±)-4′-methylmethcathinone (4-MMC, 2a) and (±)-4′-methyl-N-ethylmethcathinone (4-MEC, 2b) when they are both present in a mixture. In this paper we demonstrate, for the first time, the combination of HPLC-UV with amperometric detection (HPLC-AD) for the qualitative and quantitative analysis of 4-MMC and 4-MEC using either a commercially available impinging jet (LC-FC-A) or custom-made iCell channel (LC-FC-B) flow-cell system incorporating embedded graphite screen-printed macroelectrodes. The protocol offers a cost-effective, reproducible and reliable sensor platform for the simultaneous HPLC-UV and amperometric detection of the target analytes. The two systems have similar limits of detection, in terms of amperometric detection [LC-FC-A: 14.66 μg mL−1 (2a) and 9.35 μg mL−1 (2b); LC-FC-B: 57.92 μg mL−1 (2a) and 26.91 μg mL−1 (2b)], to the previously reported oxidative electrochemical protocol [39.8 μg mL−1 (2a) and 84.2 μg mL−1 (2b)], for two synthetic cathinones, prevalent on the recreational drugs market. Though not as sensitive as standard HPLC-UV detection, both flow cells show a good agreement, between the quantitative electroanalytical data, thereby making them suitable for the detection and quantification of 4-MMC and 4-MEC, either in their pure form or within complex mixtures. Additionally, the simultaneous HPLC-UV and amperometric detection protocol detailed herein shows a marked improvement and advantage over previously reported electroanalytical methods, which were either unable to selectively discriminate between structurally related synthetic cathinones (e.g. 4-MMC and 4-MEC) or utilised harmful and restrictive materials in their design