Sono, estresse, fadiga e funcionamento executivo do Policial Rodoviário Federal no Rio Grande do Sul.

Brazil is among the top 10 countries with the highest number of deaths from road traffic accidents. Within this scenario are the Federal Highway Police (FHP), who carry out overt patrols, seeking to ensure public safety and preservation of life. A cross-sectional observational study with an analytical descriptive approach was conducted, investigating the sleep quality, stress, fatigue, and executive functioning of these professionals, aimed at updating the institutional health intervention guidelines. We identified a prevalence of individuals with impaired sleep quality, and the presence of chronic fatigue and vulnerability to work stress, in infrastructure and routine (EVENT test). This factor relates to issues such as of working double shifts, health problems and accidents at work.  O Brasil está entre os 10 países com maior número de óbitos por acidentes de trânsito. Esse problema diz respeito ao cenário em que atuam os Policiais Rodoviários Federais (PRFs), os quais realizam patrulhamento ostensivo visando à segurança e à preservação da vida. Com o objetivo de atualizar diretrizes institucionais de intervenção em saúde para esses profissionais, passamos a investigar qualidade do sono, estresse, fadiga e funcionamento executivo, utilizando como delineamento um estudo observacional do tipo transversal com abordagem descritiva analítica. Identificamos prevalência de profissionais com comprometimento na qualidade do sono, e presença de fadiga crônica e de vulnerabilidade ao estresse no trabalho, no item infraestrutura e rotina (teste EVENT). Esse fator está relacionado a questões como dobrar jornada de trabalho, problemas de saúde e acidentes de trabalho.

Unraveling the Processing and Activation of Snake Venom Metalloproteinases

Snake venom metalloproteinases (SVMPs) are zinc-dependent enzymes responsible for most symptoms of human envenoming. Like matrix metalloproteinases (MMPs) and a disintegrin and metalloproteinase (ADAM) proteins, SVMPs are synthesized as zymogens, and enzyme activation is regulated by hydrolysis of their prodomain, but the processing of SVMPs is still unclear. In this study, we attempted to identify the presence of prodomain in different compartments of snake venom glands as zymogens or in the free form to elucidate some mechanism involved in SVMP activation. Using antibodies obtained by immunization with a recombinant prodomain, bands of zymogen molecular mass and prodomain peptides were detected mostly in gland extracts all along the venom production cycle and in the venom collected from the lumen at the peak of venom production. Prodomain was detected in secretory cells mostly in the secretory vesicles near the Golgi. We hypothesize that the processing of SVMPs starts within secretory vesicles and continues in the lumen of the venom gland just after enzyme secretion and involves different steps compared to ADAMs and MMPs but can be used as a model for studying the relevance of peptides resulting from prodomain processing and degradation for controlling the activity of metalloproteinases

Unraveling the Processing and Activation of Snake Venom Metalloproteinases

Snake venom metalloproteinases (SVMPs) are zinc-dependent enzymes responsible for most symptoms of human envenoming. Like matrix metalloproteinases (MMPs) and a disintegrin and metalloproteinase (ADAM) proteins, SVMPs are synthesized as zymogens, and enzyme activation is regulated by hydrolysis of their prodomain, but the processing of SVMPs is still unclear. In this study, we attempted to identify the presence of prodomain in different compartments of snake venom glands as zymogens or in the free form to elucidate some mechanism involved in SVMP activation. Using antibodies obtained by immunization with a recombinant prodomain, bands of zymogen molecular mass and prodomain peptides were detected mostly in gland extracts all along the venom production cycle and in the venom collected from the lumen at the peak of venom production. Prodomain was detected in secretory cells mostly in the secretory vesicles near the Golgi. We hypothesize that the processing of SVMPs starts within secretory vesicles and continues in the lumen of the venom gland just after enzyme secretion and involves different steps compared to ADAMs and MMPs but can be used as a model for studying the relevance of peptides resulting from prodomain processing and degradation for controlling the activity of metalloproteinases

A cidade de São Paulo e a era dos melhoramentos materiaes: Obras públicas e arquitetura vistas por meio de fotografias de autoria de Militão Augusto de Azevedo, datadas do período 1862-1863

The first photographs of São Paulo, taken by Militão Augusto de Azevedo between\ud 1862 and 1863, are always cited as documentary evidence of the citys backwardness,\ud deterioration, and lethargy in the mid 19th century. However, our view is that things were\ud quite different, based on a reading of prime sources dating back to that period. In fact, the\ud architecture and urban spaces of São Paulo already showed clear signs of modernization\ud between the years 1850 and 1860, as depicted in Azevedos photographs, which belong\ud to the collection of Paulista Museum and also of the Iconography and Museums Division of\ud the São Paulo Heritage Department