Unique features of Plasmids among different Citrobacter species

The _Citrobacter_ plasmids are supposed to represent the host genetic association within the living bacterial cell. The plasmids impart various beneficial characteristics to the host, helping it to retain suitable characteristics for adaptation as well as evolution. The study aims at understanding the role of prophage in influencing host functional characteristics by horizontal gene transfer or as whole plasmids. The _Citrobacter_ plasmid can be understood by analyzing many hypothetical protein sequences within its genome. Our study included 82 hypothetical proteins in 5 _Citrobacter_ plasmids genomes. The function predictions in 31 hypothetical proteins and 3-D structures were predicted for 11 protein sequences using PS2 server. The probable function prediction was done by using Bioinformatics web tools like CDD-BLAST, INTERPROSCAN, PFAM and COGs by searching sequence databases for the presence of orthologous enzymatic conserved domains in the hypothetical sequences. This study identified many uncharacterized proteins, whose roles are yet to be discovered in _Citrobacter_ plasmids. These results for unknown proteins within plasmids can be used in linking the genetic interactions of _Citrobacter_ species and their functions in different environmental conditions

Design of variability compensation architectures of digital circuits with adaptive body bias

The most critical concern in circuit is to achieve high level of performance with very tight power constraint. As the high performance circuits moved beyond 45nm technology one of the major issues is the parameter variation i.e. deviation in process, temperature and voltage (PVT) values from nominal specifications. A key process parameter subject to variation is the transistor threshold voltage (Vth) which impacts two important parameters: frequency and leakage power. Although the degradation can be compensated by the worstcase scenario based over-design approach, it induces remarkable power and performance overhead which is undesirable in tightly constrained designs. Dynamic voltage scaling (DVS) is a more power efficient approach, however its coarse granularity implies difficulty in handling fine grained variations. These factors have contributed to the growing interest in power aware robust circuit design. We propose a variability compensation architecture with adaptive body bias, for low power applications using 28nm FDSOI technology. The basic approach is based on a dynamic prediction and prevention of possible circuit timing errors. In our proposal we are using a Canary logic technique that enables the typical-case design. The body bias generation is based on a DLL type method which uses an external reference generator and voltage controlled delay line (VCDL) to generate the forward body bias (FBB) control signals. The adaptive technique is used for dynamic detection and correction of path failures in digital designs due to PVT variations. Instead of tuning the supply voltage, the key idea of the design approach is to tune the body bias voltage bymonitoring the error rate during operation. The FBB increases operating speed with an overhead in leakage power

Comparative functional genomics approach for the annotation of proteins in Unclassified Halophilic archaeon DL31

The structure, function and sub-cellular location prediction for the unknown proteins from Unclassified Halophilic archaeon DL31 were carried out for characterization of the proteins in their respective families. The 991 genes for hypothetical proteins in Halophilic archaeon DL31 chromosome were predicted by the application of computational methods and Bioinformatics web tools. The structure predictions for 206 unknown proteins were possible whereas functions were predicted in 825 protein sequences. The function prediction for the proteins were done by using Bioinformatics web tools like CDD-BLAST, INTERPROSCAN and PFAM by searching protein databases for the presence of conserved domains. The Sub-cellular location predictions were done for all the unknown proteins by using CELLO v 2.5 server. While tertiary structures were constructed using PS2 Server- Protein Structure Prediction server. This study revealed structural, functional and Sub-cellular localization of unknown proteins in Unclassified Halophilic archaeon DL31chromosome

Optimal Net-Load Balancing in Smart Grids with High PV Penetration

Mitigating Supply-Demand mismatch is critical for smooth power grid operation. Traditionally, load curtailment techniques such as Demand Response (DR) have been used for this purpose. However, these cannot be the only component of a net-load balancing framework for Smart Grids with high PV penetration. These grids can sometimes exhibit supply surplus causing over-voltages. Supply curtailment techniques such as Volt-Var Optimizations are complex and computationally expensive. This increases the complexity of net-load balancing systems used by the grid operator and limits their scalability. Recently new technologies have been developed that enable the rapid and selective connection of PV modules of an installation to the grid. Taking advantage of these advancements, we develop a unified optimal net-load balancing framework which performs both load and solar curtailment. We show that when the available curtailment values are discrete, this problem is NP-hard and develop bounded approximation algorithms for minimizing the curtailment cost. Our algorithms produce fast solutions, given the tight timing constraints required for grid operation. We also incorporate the notion of fairness to ensure that curtailment is evenly distributed among all the nodes. Finally, we develop an online algorithm which performs net-load balancing using only data available for the current interval. Using both theoretical analysis and practical evaluations, we show that our net-load balancing algorithms provide solutions which are close to optimal in a small amount of time.Comment: 11 pages. To be published in the 4th ACM International Conference on Systems for Energy-Efficient Built Environments (BuildSys 17) Changes from previous version: Fixed a bug in Algorithm 1 which was causing some min cost solutions to be misse

Bacteriophages as a model for studying carbon regulation in aquatic system

The interconversion of carbon in organic, inorganic and refractory carbon is still beyond the grasp of present environmentalists. The bacteria and their phages, being the most abundant constituents of the aquatic environment, represent an ideal model for studing carbon regulation in the aquatic system. The refractory dissolved organic carbon (DOC), a recently coined terminology from the microbe-driven conversion of bioavailable organic carbon into difficult-to-digest refractory DOC by microbial carbon pump (MCP), is suggested to have the potential to revolutionize our view of carbon sequestration. It is estimated that about 95% of organic carbon is in the form of refractory DOC, which is the largest pool of organic matter in the ocean. The refractory DOC is supposed to be the major factor in the global carbon cycle whose source is not yet well understood. A key element of the carbon cycle is the microbial conversion of dissolved organic carbon into inedible forms. The time studies of phage-host interaction under control conditions reveal their impact on the total carbon content of the source and their interconversion among organic, inorganic and other forms of carbon with respect to control source. The TOC- analysis statistics stipulate an increase in inorganic carbon content by 15-25 percent in the sample with phage as compared to the sample without phage. The results signify a 60-70 fold increase in inorganic carbon content in sample with phage, whereas, 50-55 fold in the case of sample without phages as compared with control. This increase in inorganic carbon content may be due to lysis of the host cell releasing its cellular constituents and utilization of carbon constituent for phage assembly and development. It also proves the role of phages in regulating the carbon flow in aquatic systems like oceans, where their concentration outnumbered other species

Phage-Encoded Endolysins

Due to the global emergence of antibiotic resistance, there has been an increase in research surrounding endolysins as an alternative therapeutic. Endolysins are phage-encoded enzymes, utilized by mature phage virions to hydrolyze the cell wall from within. There is significant evidence that proves the ability of endolysins to degrade the peptidoglycan externally without the assistance of phage. Thus, their incorporation in therapeutic strategies has opened new options for therapeutic application against bacterial infections in the human and veterinary sectors, as well as within the agricultural and biotechnology sectors. While endolysins show promising results within the laboratory, it is important to document their resistance, safety, and immunogenicity for in-vivo application. This review aims to provide new insights into the synergy between endolysins and antibiotics, as well as the formulation of endolysins. Thus, it provides crucial information for clinical trials involving endolysins