Evaluation of insulin resistance in adolescent girl with menstrual irregularities

Background: Polycystic ovary syndrome is most commonly recognized endocrinopathy in reproductive age group. The symptoms of PCOS vary with age, race, weight, and medications, which lead to the challenges of accurate diagnosis particularly in adolescent age group. Adolescent with PCOS most commonly encountered by gynecologist, for their menstrual irregularities. During early puberty, menstrual irregularities can occur normally due to immature Hypothalamo-pituitary-ovarian axis. Early detection of PCOS is required to control high risk of insulin resistance followed by reproductive and metabolic consequences latter on in life. Also diabetes mellitus is asymptomatic at early stage of insulin resistance and impaired glucose tolerance. Few studies have been conducted in Indian population, for prevalence of insulin resistance and metabolic syndrome in adolescents with PCOS, with varying results and hence further study is required. The aim and objectives of the study was to evaluate the presence of insulin resistance in adolescents with menstrual disorders persisting 2 years after menarche.Methods: A Cross sectional Study consisting of 102 adolescent girls done in Gynecology Outpatient Clinic of the Indira Gandhi Institute Of Medical Science Biochemical analysis was done on(day 3 to day 5) of menstruation for clinical/laboratory hyperandrogenism ,and various cause of anovulation. HOMA-IR value was evaluated for insulin resistance by following formula: fasting serum insulin (μU/ml) × fasting plasma glucose (m mol/l)/22.5. The 2003 Rotterdam consensus workshop criteria were used for ultrasonographic feature of polycystic ovarian syndrome.Results: Most common pattern of menstrual irregularities was Oligomenorrhea. 4 (7.84%) cases had insulin resistance (HOMA-IR >3.9) and 25 (49.01%) cases had elevated HOMA-IR values (HOMA-IR >1). PCOS was seen in 35.29% of cases and only 1.96% of control.Conclusions: Adolescents with persistent menstrual irregularities even after two year of menarche, especially with oligomenorrhea and hypomenorrhoea, more frequently have the diagnosis of PCOS and also present with elevated HOMA-IR values

Evaluation of Antimicrobial and Antifungal efficacy of Chitosan as endodontic irrigant against Enterococcus Faecalis and Candida Albicans Biofilm formed on tooth substrate

Bacterial biofilms formed on the root canal wall are often difficult to remove. This study aimed to evaluate the cytotoxic effect and antibacterial efficacy of chitosan when used as root canal irrigant against E. Faecalis and Candida albicans biofilm formed on tooth substrate. The present study evaluated antibacterial effect of 0.25% Chitosan, 0.5% Chitosan, 2% chlorhexidine and 3% sodium hypochlorite against Enterococcus faecalis and Candida Albicans. Agar-well diffusion methods, minimal inhibitory concentration tests and biofilm susceptibility assays were used to determine antibacterial activity. Teeth specimens were sectioned to obtain a standardized tooth length of 12mm. Specimens were inoculated with 10 mL of the freshly prepared E. Faecalis suspension and Candida albicans for 4 weeks. The specimens were then instrumented with ProTaper rotary files F3 size. After irrigation with test solution, three sterile paper points were placed into one canal, left for 60 s and transferred to a test tube containing 1 mL of reduced transport fluid. The number of CFU in 1 mL was determined. 3-week biofilm qualitative assay showed complete inhibition of bacterial growth with 3% Sodium hypochlorite, 2% Chlorhexidine and Chitosan except saline, which showed presence of bacterial growth. Significant reduction of colony forming units (CFU)/mL was observed for the chitosan groups and the antibacterial activity of the chitosan groups was at par with 3% NaOCl and 2% Chlorhexidine. It was observed that the chitosan showed no cytotoxicity at 3mg/ml and 10% cytotoxicity at 6mg/ml. The use of chitosan as a root canal irrigant might be an alternative considering the various undesirable properties of NaOCl and chlorhexidine

Influence of root-knot nematode, Meloidogyne incognita (Kofoid & White) Chitwood infection on different plant growth parameters in Mungbean, Vigna radiata (L.) Wilczek

351-359Root-knot nematode, Meloidogyne incognita (Kofoid & White) Chitwood, is a major threat to mungbean cultivation. The pest causes a significant reduction in plant growth parameters that ultimately results in loss of grain yield. The present study was carried out under glass house condition to study the effect of different inoculum load of root-knot nematode M. incognita on plant growth, nodulation and nematode development and nutrients status of Mungbean. The results revealed a progressive decline in plant growth parameters viz., fresh and dry shoot weight and shoot length with respect to increase in inoculum level. However, fresh and dry root weight showed the opposite trend. The fresh and dry shoot weight was decreased by 44% and 66%, respectively at 4 J2s/g soil. The chlorophyll content in the leaves also decreased with the increase of inoculum level from 100-6000 J2s/pot. Nutrients contents of the plant viz. N, P, K, Ca and Mg were significantly reduced in shoots while in roots these was increased with an increase of inoculum levels. Nodulation was affected by 80% at the highest inoculum level i.e. 6000 J2s/pot. Also leghaemoglobin, bacteroid content and nitrogenase activity was reduced progressively with increased levels of nematode inoculum. Thus, the root-knot nematode, M. incognita interferes with the process of symbiotic nitrogen fixation between mungbean host and rhizobium and that can affect the quality of produce

Serum BUN and creatinine estimation in patients of overt hypothyroidism: a case control study

Background: Hypothyroidism or underactive thyroid or low thyroid is a common endocrine disorder characterized by low serum T3 (triiodothyronine), T4 (thyroxine) and raised TSH (thyroid stimulating hormone). Thyroid hormones are involved in renal development and hemodynamic, kidney structure and GFR (glomerular filtration rate). Aim of this study was to see the alteration of basic renal markers in patients of hypothyroidism.Methods: A total of seventy subjects were included in the study. Thirty-five were patients of hypothyroidism and thirty-five were age and sex matched normal controls. Serum T3, T4, TSH, creatinine and BUN (blood urea nitrogen) were estimated in both groups.Results: Serum T3, T4 were significantly decreased and TSH was significantly raised among cases as compared to controls. Mean value of serum creatinine and BUN were within normal range in both the groups but these values were significantly raised among cases as compared to controls with p value 0.02 and 0.003 respectively. Also, there was positive correlation of TSH with BUN and creatinine among cases

Effect of crop residue addition on soil organic carbon priming as influenced by temperature and soil properties

Priming of soil organic carbon (SOC) is a crucial factor in ecosystem carbon balance. Despite its increasing importance in the changing global climate, the extent of influence of temperature and soil properties on the priming effect remains unclear. Here, soil priming was investigated using 13C labeled wheat residues in two cultivated, subtropical (Vertisol) and semi-arid (Luvisol), soils of Australia at four incubation temperatures (13, 23, 33 and 43 °C). The priming effect was computed from respired CO2 and associated δ13C, which were measured periodically over the 52-day incubation period. Wheat residue addition resulted in greater priming effect in the Luvisol (1.17 to 2.37% of SOC) than the Vertisol (0.02 to 1.56% of SOC). The priming of SOC was the highest at 23 °C in the Luvisol, and at 43 °C in the Vertsiol, which indicates a variable positive priming effect of temperature in different soil types. Wheat residue addition significantly increased the temperature sensitivity (Q10) of SOC mineralization in the Vertisol at temperature ranges below 33 °C (i.e., 13–23 and 23–33 °C) and had no significant effect in the Luvisol. A negative correlation was observed between temperature and the Q10 values. Across soils, the Q10 of residue C was lower than SOC suggesting that soil C is more vulnerable to climatic warming. This work demonstrates that the magnitude of SOC priming by wheat residue and Q10 of SOC mineralization varied significantly with soil type (Luvsiol > Vertisol) and incubation conditions (temperature and time). Given the current trend towards increasing atmospheric temperatures, future studies should evaluate temperature effects on the priming of different pools of SOC induced by crop residue in different agro-ecosystems

Analysis of noise pollution level in and around SIDCUL area in District Haridwar (Uttarakhand) India

The primary objective of this study is to evaluate the issue of noise pollution in the vicinity of the industrial zone of Haridwar city and its correlation with the health of the people in and around the SIDCUL (State Industrial Development Corporation of Uttarakhand Limited). The study revealed that noise pollution levels were above the CPCB Standard for the sound level for industrial zones and residential zones. During the period from January to December 2018, noise levels were observed and monitored in both the industrial zone (Site-I SIDCUL) and the residential zone (Site-II Siwalik Nagar). The average noise levels varied throughout the year. At Site-I (Industrial Zone), during the daytime in July, the maximum average noise level recorded was 89.5 dB, while in April; the minimum average noise level was 81.1 dB. During nighttime, in January, the maximum average noise level reached 84.1 dB, and in May, the minimum average noise level was 76.6 dB. In Site-II Siwalik Nagar (Residential Zone), the daytime noise levels were a maximum average noise level of 61.1 dB in May and a minimum average noise level of 58.8 dB in September. During nighttime, the noise level reached a maximum average of 47.2 dB in October and a minimum average of 44.5 dB in May. Overall, the study revealed that noise levels were generally higher in both zones, except for the average nighttime noise level at Site II (residential zone), which was below the prescribed standard limit for noise