Student’s perception about teaching methodologies used in pharmacology: a questionnaire based cross sectional study

Background: Pharmacology is one of the most clinically applied subjects which are highly volatile. Hence new methodologies have to be introduced in medical curriculum which must be in line with the students’ preferences. The study aimed to determine the opinion of students regarding the teaching of pharmacology, the best way of knowing and retaining the subject and application of the subject in future practice.Methods: The study will be conducted at private Medical college in Mangalore. It is an observational, KAP cross-sectional questionnaire-based study. Convenient sampling method is used in which 140 Second year MBBS students were enrolled. SPSS version 21 was used to generate tables and graphs. Results of the study are based on descriptive statistics.Results: Questions which were not answered were taken as invalid response. Results show that majority preferred newer learning methodology, case-based learning, integrated teaching were more appealing, and peer associated teaching like group discussion was better for applied topics. Attitude of students in learning has shifted from traditional text book to the recent concept of knowledge sharing.Conclusions: In general students’ perceptions regarding learning pharmacology was observed to be positive. The study highlights the need for interactive teaching. Practical sessions can be used to teach basic concepts and rational use of drugs. Knowledge seeking behaviour needs improvisation by teacher

Knowledge, attitude and practice of medical professionals towards adverse drug reaction reporting and pharmacovigilance in a tertiary care hospital: a cross sectional study

Background: The present study was planned to assess the knowledge, attitude and practice of healthcare providers regarding adverse drug reaction reporting and pharmacovigilance in a tertiary care hospital.Methods: The study was conducted in a tertiary care teaching hospital, it’s a observational, KAP cross-sectional questionnaire-based study. The KAP questionnaires was developed toward pharmacovigilance and ADRs and were used to assess the medical professionals.Results: Only 64% of doctors, 52% PG’s and 40% of nurses knew the correct knowledge regarding Pharmacovigilance (PhV). Regarding the attitude, all the respondents think reporting of ADR is a very necessary. 98% of doctors,80% of postgraduates and 96% of nurses have experienced ADR in the patient in their professional practice but reporting of same is very less. The factors discouraging them from reporting ADR’s was also assessed. 34% said difficult to decide whether ADR has occurred or not, 34% said lack of time, 17%- no remuneration and 15% said a single unreported case may not affect ADR database.Conclusions: This study demonstrated that knowledge and attitude towards pharmacovigilance is gradually improving among medical professionals, but unfortunately the actual practice of ADR reporting is still deficient among them

New liver cell mutants defective in the endocytic pathway

AbstractTo isolate mutant liver cells defective in the endocytic pathway, a selection strategy using toxic ligands for two distinct membrane receptors was utilized. Rare survivors termed trafficking mutants (Trf2–Trf7) were stable and more resistant than the parental HuH-7 cells to both toxin conjugates. They differed from the previously isolated Trf1 HuH-7 mutant as they expressed casein kinase 2 α″ (CK2α″) which is missing from Trf1 cells and which corrects the Trf1 trafficking phenotype. Binding of 125I-asialoorosomucoid (ASOR) and cell surface expression of asialoglycoprotein receptor (ASGPR) were reduced approximately 20%–60% in Trf2–Trf7 cells compared to parental HuH-7, without a reduction in total cellular ASGPR. Based on 125I-transferrin binding, cell surface transferrin receptor activity was reduced between 13% and 88% in the various mutant cell lines. Distinctive phenotypic traits were identified in the differential resistance of Trf2–Trf7 to a panel of lectins and toxins and to UV light-induced cell death. By following the endocytic uptake and trafficking of Alexa488-ASOR, significant differences in endosomal fusion between parental HuH-7 and the Trf mutants became apparent. Unlike parental HuH-7 cells in which the fusion of endosomes into larger vesicles was evident as early as 20 min, ASOR endocytosed into the Trf mutants remained within small vesicles for up to 60 min. Identifying the biochemical and genetic mechanisms underlying these phenotypes should uncover novel and unpredicted protein–protein or protein–lipid interactions that orchestrate specific steps in membrane protein trafficking

Dynamic actin-mediated nano-scale clustering of CD44 regulates its meso-scale organization at the plasma membrane

Transmembrane adhesion receptors at the cell surface, such as CD44, are often equipped with modules to interact with the extracellular matrix (ECM) and the intracellular cytoskeletal machinery. CD44 has been recently shown to compartmentalize the membrane into domains by acting as membrane pickets, facilitating the function of signaling receptors. While spatial organization and diffusion studies of membrane proteins are usually conducted separately, here we combine observations of organization and diffusion by using high spatio-temporal resolution imaging on living cells to reveal a hierarchical organization of CD44. CD44 is present in a meso-scale meshwork pattern where it exhibits enhanced confinement and is enriched in nanoclusters of CD44 along its boundaries. This nanoclustering is orchestrated by the underlying cortical actin dynamics. Interaction with actin is mediated by specific segments of the intracellular domain. This influences the organization of the protein at the nano-scale, generating a selective requirement for formin over Arp2/3-based actin-nucleation machinery. The extracellular domain and its interaction with elements of ECM do not influence the meso-scale organization, but may serve to reposition the meshwork with respect to the ECM. Taken together, our results capture the hierarchical nature of CD44 organization at the cell surface, with active cytoskeleton-templated nanoclusters localized to a meso-scale meshwork pattern

Identification of Fluorescent Compounds with Non-Specific Binding Property via High Throughput Live Cell Microscopy

10.1371/journal.pone.0028802PLoS ONE71

Cell adhesion by aqueous extract of human placenta used as wound healer

732-738Aqueous extract of human placenta, used as wound healer, has shown significant cell adhesion property on mouse peritoneal macrophages and P388D1 cultured macrophage cell line. This property was offered primarily by fibronectin type III like peptide present in the extract and is comparable to fibronectin on a molar basis. The peptide induce adhesion of cell through cell surface receptors having Kd = 2.8±0.9 x 10-5 M suggesting weak binding. This is in support of integrins receptors that typically exhibit low affinities. Cell adhesion was partially inhibited by Arg-Gly-Asp (RGD) peptide, anti-β1 integrin suggesting that integrin β1 receptors have roles to play in the process

Mild electrical stimulation of pontine tegmentum around locus coeruleus reduces rapid eye movement sleep in Rats

The norepinephrinergic neurons in the locus coeruleus (LC) cease firing during REM sleep (REMS) and increase firing during REMS deprivation. Most of the earlier studies used lesion and transection techniques which could not confirm the role of LC in REMS generation and/or its maintenance, if at all. Hence, in this study it was hypothesized that if the LC REM-off neurons must cease firing before the onset of REMS, its continuous activation should eliminate or at least reduce REMS. Electrophysiological parameters characterizing sleep-wakefulness-REMS were recorded in freely moving male albino rats. In an attempt not to allow the REM-off LC neurons to cease firing, low intensity (200,μA), low frequency (2 Hz) rectangular (300, µs) pulses were continuously delivered to the LC bilaterally through chronically implanted electrodes, and the effects on sleep-wakefulness-REMS were investigated. Although the stimulation did not affect sleep state of the animals, it reduced REMS significantly. The effect on REMS was similar to that of REMS deprivation. Total duration of REMS was significantly reduced during stimulation and showed a rebound increase during the post stimulation period. This reduction in REMS duration was primarily due to a significant reduction in the REMS frequency/h while the mean REMS duration/episode was not affected. Thus, the results of this study suggest that the stimulated area (LC) affects REMS, most likely by suppression of REMS generation process

Dynamics of Mikir hills plateau and its vicinity: Inferences on Kopili and Bomdila Faults in Northeastern India through seismotectonics, gravity and magnetic anomalies

he Mikir Hills plateau is encompassed by two prominent faults – Bomdila Fault to the east and the Kopili Fault to the west characterized by strike-slip kinematics. The Kopili Fault has a dip of 75otowards NE. Simultaneously, the Bomdila Fault dips with 50-55otowards the NNE. An integrated approach based on seismotectonics, gravity and magnetic data is utilized to understand the tectonic activity of the Kopili and Bomdila Faults. The bottom of seismogenic zones is inferred to be 45+2 km and 50+2 km for the Kopili Fault and the Bomdila Fault region respectively. So far gravity anomaly is concerned; it varies between -110 to +60 mgals along the Kopili –Bomdila Fault regions from the Belt of Schuppen to the MCT. The low gravity values over the Bomdila Fault area indicate presence of thick alluvial deposits while along Kopili Fault lesser sediment thickness is observed. Simultaneously, basement being at shallower depth, lower magnetic values indicate presence of thick alluvial deposits in and around Bomdila Fault. The curvatures and closures of the gravity contours along the fault lines indicate structures involving basement and indicate influence of Bomdila Fault up to the basement. Simultaneously, it is observed that Kopili and Bomdila faults are neotectonically active. All these are the prime input to the seismic hazard assessment of the region