Liming and application of nitrogen, phosphorus, potassium, and boron on a young plantation of chestnut

European chestnut is the most important cash crop in the highlands of NE Portugal. However, limited data on cropping technique are available to help farmers make decisions. This work is motivated by the lack of data related to chestnut response to fertilizer application. It reports results from a field fertilization trial consisting of the application of a combination of lime (L), nitrogen (N), phosphorus (P), potassium (K), and boron (B), namely LNPKB, NPKB, –NPKB, N–PKB, NP–KB, and NPK–B. Seedlings of chestnut were planted in June 2013 and grown for four growing seasons. Plant height, trunk diameter, and pruning wood were assessed every year and used as indicators of tree crop growth. Elemental leaf analysis and other tools [SPAD-502 chlorophyll meter; Field Scout CM 1000 NDVI (normalized difference vegetation index) meter, and the OS-30p+ fluorometer] were used to assess plant nutritional stresses. In January 2017 soil samples were collected at three depths (0–5, 5–10, and 10–20 cm) to determine several soil fertility parameters. The nonapplication of K and B reduced the tree crop growth and pruning residues. Leaf analysis revealed leaf K and B concentrations respectively in the NP–KB and NPK–B treatments to be in the deficient range previously established for chestnut. SPAD readings and NDVI detected the reduced availability of N associated with the –NPKB treatment but not the reduced plant growth associated with NP–KB or NPK–B treatments. The dark adaptation protocols of FV/FM, FV/F0, and OJIP measurements performed with the OS-30p+ fluorometer failed to diagnose the nutrient stresses detected by leaf analysis or the reduction in plant growth.info:eu-repo/semantics/publishedVersio

Estudos de fertilização em castanheiro com aplicação de calcário, azoto, fósforo, potássio ou boro

Este trabalho foi motivado pela falta de informação disponível sobre resposta do castanheiro à correção do pH e à aplicação de outros fertilizantes ao solo, numa fase em que, face aos bons preços a que se vende a castanha, os produtores têm estado muito predispostos a investir na técnica cultural. O trabalho reporta resultados de um ensaio em castanheiro que decorreu durante quatro anos, onde se ensaiaram seis modalidades de fertilização: aplicação de calcário mais azoto (N), fósforo (P), potássio (K) e boro (B) (CalNPKB); aplicação de N, P, KeB (NPKB); aplicação de P, Ke B (-NPKB); aplicação de N, K e B (N-PKB); aplicação de N, Pe B (NP-KB); e aplicação de N, Pe K (NPK-B). Os tratamentos fertilizantes foram aplicados à instalação das jovens plantas no ano de plantação e mantidos por quatro anos consecutivos, com exceção do calcário que só foi aplicado à instalação. Foi avaliada a resposta das plantas aos tratamentos fertilizantes através da medição da sua altura e do diâmetro do tronco e da lenha de poda produzida, bem como do estado nutricional das plantas avaliado a partir de análises elementares às folhas e com aparelhos portáteis. As plantas dos tratamentos NPK-B, NP-KB e -NPKB revelaram, respetivamente, teores de B, Ke N significativamente mais baixos que os restantes tratamentos. O tratamento N-PKB não revelou teores de P mais baixos que os restantes e o tratamento CalNPKB não revelou teores de cálcio ou magnésio superiores aos restantes. A altura das plantas e o diâmetro dos caules foram significativamente mais baixos nas modalidades NPKB e NP-KB. Os resultados dos aparelhos portáteis de medição da condição das plantas confirmaram que a falta de B e K foram os tratamentos que mais reduziram o estado nutricional das plantas nos respetivos elementos e maior influencia negativa exerceram no crescimento em altura e engrossamento das árvores. Recomenda-se que as futuras recomendações de fertilizaçâo para esta cultura tenha estes dois elementos em conta.info:eu-repo/semantics/publishedVersio

Método del papel de filtro para la medida de la succión del suelo

The capillary pressure of the soil (i.e., the pressure difference between air and water components in soil voids) is a key variable in the analysis of the hydro-mechanical behavior of unsaturated soils. Therefore a simple and economical laboratory method for the measurement of the capillary pressure of the soil (also known as soil matric suction, the reference being the atmospheric pressure), even if a degree of approximation is involved, is of considerable value. The filter paper method calculates soil suction indirectly by measuring the gravimetric water content of the filter paper at equilibrium that is related to soil suction through a predetermined calibration curve. The advantages of the method are simplicity, economy and reasonable accuracy. It can be used to measure suctions from 10 to 30000 kPa. In this paper, the authors use the contact filter paper method for matric suction measurements of an unsaturated compacted silty sand (formed by the weathering of granite) which has been used as a building material for a road in the north of Portugal. The matric suctions inferred from filter paper measurements depend on the calibration between the water content of the filter paper and suction. Therefore, three calibration curves proposed at the literature (Chandler et al. 1992; ASTM D 5298; and Oliveira & Marinho 2006) for the Whatman 42 filter paper are used to interpret the measured filter paper gravimetric water contents. The results of these tests are compared to other techniques (i.e., tensiometers, and the osmotic technique) used to measure or control the negative pore water pressure in the compacted soil specimens and the results obtained are reasonably accurate.La presión capilar del suelo (es decir, la diferencia de la presión entre el aire y los componentes del agua en vacíos del suelo) es una variable llave en el análisis del comportamiento hidromecánico de suelos no saturados. Un método por lo tanto simple y económico del laboratorio para la medida de la presión capilar del suelo (también conocido como la succión matrica del suelo, la referencia que es la presión atmosférica), mesmo si un grado de aproximación está implicado, es de valor considerable. El método del papel de filtro calcula la succión indirectamente utilizando curvas de calibración. Las ventajas del método son simplicidad, economía y exactitud razonable. El método del papel de filtro se puede utilizar para medir succiones a partir del 10 al 30000 kPa. En este artículo, los autores utilizan el método del papel de filtro para la medida de la succión matric de una arena limosa compactada no saturada (formada por la meteorización del granito) que se ha utilizado como material de construcción para un camino en el norte de Portugal. Las succiones matric deducidas de medidas del papel de filtro dependen de una calibración entre el humedade del papel de filtro y la succión. Por lo tanto, tres curvas de calibración propuestas en la literatura (Chandler et al. 1992; ASTM D 5298; y Oliveira & Marinho 2006) para el papel de filtro de Whatman 42 se utilizan para interpretar lãs humedades gravimétricas medidas del papel de filtro. Los resultados de los ensayos se comparan a otras técnicas (es decir, tensiómetros, y la técnica osmótica) usadas para medir o controlar la presión negativa en lãs muestras compactadas del suelo y los resultados obtenidos sea razonablemente exacto

Efeito da calibração do papel filtro na curva de retenção de um solo arenoso siltoso compactado

The soil suction is a key variable in the analysis of the hydro-mechanical behavior of unsaturated soils. The filter paper method (FPM) calculates soil suction indirectly by measuring the gravimetric water content of the filter paper at equilibrium that is related to soil suction through a predetermined calibration curve. The matric suctions inferred from FPM depend on the calibration between the water content of the filter paper and suction. Therefore, some published calibration curves (Fawcett and Collis-George 1967; Hamblin 1981; Chandler and Gutierrez 1986; Chandler et al. 1992; ASTM D-5298 1992; and Oliveira and Marinho 2006) for the Whatman 42 filter paper are used to interpret the suction measurements of an unsaturated compacted silty sand. Experimental errors induced by using an inadequate calibration curve are discussed. The test results compared to other techniques used to measure or control suctions in the compacted soil specimens are reasonably accurate.A sucção de um solo é uma variável essencial na análise do comportamento de solos não saturados, e é necessário e importante o desenvolvimento de técnicas, diretas e indiretas, de determinação da pressão capilar de solos, que tentam aliar à simplicidade de aplicação, o que se espera ser uma precisão aceitável para os problemas reais do dia a dia da engenharia de solos. O MPF determina a sucção de forma indireta e depende da precisão em que foi determinada a curva de calibração. Várias curvas de calibração para o papel filtro Whatman 42 têm sido propostas na literatura (Fawcett and Collis-George 1967; Hamblin 1981; Chandler and Gutierrez 1986; Chandler et al. 1992; ASTM D-5298 1992; e Oliveira and Marinho 2006). Este artigo discute o uso do MPF para determinar valores de sucção de um solo arenoso siltoso compactado não saturado. Comparam-se os resultados obtidos com outras técnicas utilizadas para medir ou controlar sucções de amostras compactadas do solo arenoso siltoso e conclui-se que o MPF com adequada curva de calibração pode apresentar resultados satisfatórios.Universidade de Coimbra. Faculdade de Ciências e Tecnologia (FCT-UC)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES

NEP-TC a rRNA methyltransferase involved on somatic embryogenesis of tamarillo (Solanum betaceum cav.)

Somatic embryogenesis (SE) is an important biotechnological tool for large-scale clonal propagation and for embryogenesis research. Moreover, genetic transformation and cryopreservation procedures in many species rely on efficient SE protocols. We have been studying different aspects related to SE induction and somatic embryo development in tamarillo (Solanum betaceum Cav.), a small tree from the Solanaceae family. Previous proteomic analyses identified a protein (NEP-TC, 26.5 kDa) consistently present in non-embryogenic calluses of tamarillo, but absent in the embryogenic ones. In this work, the role of NEP-TC during SE was assessed by gene expression analysis and immunolocalization. The results obtained demonstrated that NEP-TC is a putative member of the SpoU rRNA methylase family. This protein, present in the cytoplasm and nucleus, is expressed in non-embryogenic cells and not expressed in embryogenic cells. Slightly enhanced SE induction levels in tamarillo plants with NEP-TC down-regulated levels also supports the role of this protein on SE induction. Heterologous expression was used to confirm NEP-TC rRNA methyltransferase activity, with enhanced activity levels when rRNA was used as a substrate. These data relate a putative member of the SpoU methylase family with plant morphogenesis, in particular with SE induction.publishe

Arbuscular mycorrhizal fungi inoculation reduced the growth of pre-rooted olive cuttings in a greenhouse

The effect of commercial mycorrhizal fungi on pre-rooted olive cuttings was assessed. The study consisted of two experiments, the first arranged as a factorial design with three cultivars (Cobrançosa, Madural, and Verdeal Transmontana) and three soil treatments (commercial mycorrhizal fungi, zeolites, and control) and the second as a completely randomized design with three treatments (commercial mycorrhizal fungi, sterilized soil, and control). Cobrançosa grew better than the other cultivars, showing good rooting and initial growth features. Mycorrhizal plants showed reduced growth in comparison to those of the untreated control. This result was explained by competition for photosynthates between plant growth and the expansion of fungi hyphae. Cuttings of reduced leaf area and a twin-wall polycarbonate cover of the greenhouse, somewhat opaque to photosynthetic active radiation, may also have contributed to limit the maximum photosynthetic rate and delay the growth of the inoculated plants. Accordingly, in the mycorrhizal pots, the soil organic carbon (C) increased, probably due to the presence of fungi hyphae in soil samples. Zeolites reduced plant dry matter (DM) yield and tissue phosphorus (P) concentration compared to the control, while increasing the availability in the soil of the cations present in their initial composition. Soil sterilization seems to have reduced soil P bioavailability by inactivating soil enzymes. This study showed that the inoculation with mycorrhizal fungi of pre-rooted cuttings can delay their initial growth. Although these plants may be better prepared to grow in the field, in the short term, their lower development can be a problem for the nurseryman.This research was funded by the Foundation for Science and Technology (FCT, Portugal) and FEDER under Programme PT2020 for financial support to CIMO (UIDB/00690/2020) and CITAB (UIDB/04033/2020). The research was integrated in the activities of the operational group “Novas práticas em olivais de sequeiro: estratégias de mitigação e adaptação às alterações climáticas”, funded by PT2020 and EAFRD (European Agricultural Fund for Rural Development).info:eu-repo/semantics/publishedVersio

Posterior talar process as a suitable cell source for treatment of cartilage and osteochondral defects of the talus

Osteochondral defects of the ankle are common lesions affecting the talar cartilage and subchondral bone. Current treatments include cell-based therapies but are frequently associated with donor-site morbidity. Our objective is to characterize the posterior process of the talus (SP) and the os trigonum (OT) tissues and investigate its potential as a new source of viable cells for application in tissue engineering and regenerative medicine.SP and OT tissues obtained from six patients were characterized by micro-computed tomography, and histological, histomorphometric and immunohistochemical analyses. Isolated cells proliferation and viability were evaluated by MTS assay, DNA quantification and Live/Dead staining. The TUNEL assay was performed to evaluate cell death by apoptosis. Moreover, the production of extracellular matrix was evaluated by toluidine blue staining, whereas cells phenotype was investigated by flow cytometry. Ankle explants characterization showed the presence of a cartilage tissue layer in both SP and OT tissues, which represent, at least 20% in average of the explant. The presence of type II collagen was detected in the extracellular matrix. Isolated cells presented a round morphology typical of chondrocytes. In in vitro studies, cells were viable and proliferating up to 21 days of culturing. No signs of apoptosis were detected. Flow cytometry analysis revealed that isolated cells maintained the expression of several chondrocytic markers during culturing. The results indicate that the SP and OT tissues are a reliable source of viable chondrocytes, which can find promising applications in ACI/MACI strategies with minimal concerns regarding donor zone complications.Portuguese Foundation for Science and Technology (FCT) through the project OsteoCart (Grant No. PTDC/CTM-BPC/115977/2009), Fundación MAPFRE (Ayudas a la Investigación Ignacio H. de Larramendi, Prevención, Salud y Medio Ambiente, Spain) under the project 'Preventing the progression of the knee osteoarthritis: advanced therapies combining injectable hydrogels, autologous stem cells and PRP' (Grant No. BIL/13/SA/235). This study was also carried out with the support of Fundo Europeu de Desenvolvimento Regional (FEDER) through Programa Operacional do Norte through the project Articulate (Grant No. 23189

Olive yield and physicochemical properties of olives and oil in response to nutrient application under rainfed conditions

The effects of mineral fertilizers on the physicochemical properties of olives and oil under rainfed conditions is scarce. In this three-year study, the results of a nitrogen (N), phosphorus (P), potassium (K) and boron (B) fertilization trial carried out in a young rainfed olive grove and arranged as a nutrient omission trial are reported. The control consisted of the application of N, P, K and B (NPKB) and four other treatments corresponded to the removal of one of them (N0, P0, K0 and B0). Olive yield and several variables associated with the physicochemical properties of olives and oil were evaluated. The NPKB treatment increased olive yield compared to the treatment that did not receive N (N0). Although dependent on the climate conditions of the crop season, the NPKB treatment increased fruit weight and the pulp/pit ratio and its fruits tended to accumulate more oil than K0. However, the phenolics concentrations on fruits and oil tended to be lower. All olive oil samples were classified in the “extra virgin” category and all showed a decrease in its stability between 3 and 15 months of storage, regardless of treatment, especially in N0, P0 and B0 treatments. The results of the sensorial analysis indicate that all the oils fell into the medium fruitiness and greenly-fruity category. Only in P0 and B0 were defects detected, namely muddy sediment. Thus, this study seems to indicate the importance of N application, but also a balanced nutrient application and that further studies are needed, given the difficulty in finding clear trends in the response of measured variables to fertilizer treatments.Ermelinda Silva acknowledges the financial support provided by national funds through FCT -Portuguese Foundation for Science and Technology (PD/BD/128274/2017), under the Doctoral Programme “Agricultural Production Chains—from fork to farm” (PD/00122/2012) and from the European Social Funds and the Regional Operational Programme Norte 2020. This research was funded by the Operational Group “Novas práticas em olivais de sequeiro: estratégias de mitigação e adaptação às alterações climáticas”, funded by PT2020 and EAFRD (European Agricultural Fund for Rural Development). The authors are grateful to the Foundation for Science and Technology (FCT, Portugal) and FEDER under Programme PT2020 for financial support to CIMO (UIDB/00690/2020) and CITAB (UIDB/04033/2020) and also to AgriFood XXI Project, No. NORTE-01-0145-FEDER- 000041, co-financed by the European Regional Development Fund (ERDF) through NORTE 2020 (North Regional Operational Program 2014–2020). Ermelinda Silva also acknowledges the present financial support throughout project NORTE-06-3559-FSE-000103 funded by the Fundo Social Europeu (FSE) and FCT research unit Centre BIO R&D Unit (UIDB/05083/2020).info:eu-repo/semantics/publishedVersio

Leguminous cover crop to adapt olive rainfed orchards to climate change

Leguminous cover crops have the potential to contribute to soil protection against erosion and improve carbon sequestration, soil and water quality and might help agricultura! systems become more resilient to climate change. The aim of this study was to investigate the effects of a cover crop of self-reseeding annuallegumes of short growing cycle on physiological and biochemical responses of twenty-six-year-old olive trees (Olea europaea L. cv. Cobrançosa) grown under rainfed conditions, when compared with conventional tillage. The results showed that the leguminous cover crop ameliorates the physiological and biochemical performance of olive tree during the drought season, judging by the rise of net photosynthesis, transpiration rate and stomatal conductance, whereas the opposite pattern was observed for the concentration of total phenols and for total antioxidant activity in leaves. ln addition, it was observed an increase on yield, size and weight of olive fruits. These results indicate that the use of well-designed, locally-tailored cover crop mixtures, associated with an appropriate mowing time, is a very promising strategy to implement in olive orchards under water scarcity. Thus, cover cropping is a win-win strategy as it increases farm benefits and provides ecosystem services.This work was funded by the INTERACT project- "Jntcgrative Research in Environment, Agro-Chains and Technology", no. NORTE-01-0145-FEDER-000017, in its !ines of research entitled ISAC, co-tinanced by the European Regional Devclopment Fund (ERDF) through NORTE 2020 (North Regional Operational Program 20 14/2020). SM (PD/BD/135327/2017), EM (PD/BD/128274/2017) and CB (PD/BD/52543/2014) acknowledge the financ ial support provided by the FCT-Portuguese Foundatíon for Science and Technology, under the Doctoral Programme "Agricultura] Production Chains- from fork to farm" (PD/00122/2012).info:eu-repo/semantics/publishedVersio

Natural-origin materials for tissue engineering and regenerative medicine

Recent advances in tissue engineering and regenerative medicine have shown that combining biomaterials, cells, and bioactive molecules are important to promote the regeneration of damaged tissues or as therapeutic systems. Natural origin polymers have been used as matrices in such applications due to their biocompatibility and biodegradability. This article provides an up-to-date review on the most promising natural biopolymers, focused on polysaccharides and proteins, their properties and applications. Membranes, micro/nanoparticles, scaffolds, and hydrogels as biomimetic strategies for tissue engineering and processing are described, along with the use of bioactive molecules and growth factors to improve tissue regeneration potential. Finally, current biomedical applications are also presented.The authors would like to thank to the financial support from the Portuguese Foundation for Science and Technology (FCT) for the fellowship grants of Simone S Silva (SFRH/BPD/112140/2015), Emanuel M Fernandes (SFRH/BPD/96197/2013), Joana-Silva Correira (SFRH/BPD/100590/2014), Sandra Pina (SFRH/BPD/108763/2015), Silvia Vieira (SFRH/BD/102710/2014), “Fundo Social Europeu”- FSE and “ Programa Diferencial de Potencial Humano POPH”, and to the distinction attributed to J.M. Oliveira under the Investigator FCT program (IF/00423/2012). It is also greatly acknowledged the funds provided by FCT through the project EPIDisc (UTAP-EXPL/BBBECT/0050/2014), financed in the Framework of the “International Collaboratory for Emerging Technologies, CoLab”, UT Austin|Portugal Program.info:eu-repo/semantics/publishedVersio