Ocorrência de rota vírus e adeno vírus em crianças de até 11 anos de idade sem sintomatologia de diarréia em Goiânia - GO

Trezentas e oitenta e cinco amostras fecais provenientes de crianças na faixa etária de até 11 anos, sem sintomatologia de diarréia, foram estudadas objetivando-se a detecção de rotavirus. Desta amostragem, 268foram obtidas de crianças habitantes de creches e 117 de crianças atendidas no ambulatório do Hospital Lúcio Rebelo de Goiânia-Goiás. Todas as amostrasforam analisadas através da técnica de eletroforese em gel de poliacrilamida (EGPA- SDS), e 89 foram também analisadas pelo ensaio imunoenzimático adaptado para rotavirus e adenovirus (E1ARA). Rotavirus e adenovirus só foram detectados nas crianças atendidas no ambulatório, num percentual de 1,7% e 1,6% respectivamente, não havendo nenhuma positividade nas crianças de creches. Ambos os vírus ocorrerarh na faixa etária de 1 a 2 anos.<br>Three hundred and eigthy five children without a diagnosis of diarrhoeal illness had faecal specimens collected in order to detect rotavirus infection. The survey was conducted in Goiânia city - CentralBrazil and the children aged 0-11 years old were recruited (268 in day care centers and 117 from a local outpatient clinic - Hospital Lucio Rebelo). Detection of rotavirus was done by Polyacrylamide gel electrophoresis (PAGE - SDS), and 89 specimens were also analyzed by commercial enzyme immunoassays for rotavirus and adenovirus (EIARA). Rotaviruses and adenoviruses were only detected among the children attending the outpatient clinic. Prevalence rates of rotavirus and adenovirus excretion were of 1.7% and 1.6%, respectively. Both viruses were found among children from 1 to 2 years old

Rotavirus e adenovirus em crianças de 0-5 anos hospitalizadas com ou sem gastrenterite em Goiânia - GO., Brasil Rotavirus and adenovirus in children 0-5 years of age with or without gastrenteritis in hospitals from Goiânia - GO, Brazil

De junho/1987 a julho/1990 foram estudadas 557 amostras fecais de crianças hospitalizadas de 0-5 anos de idade, na cidade de Goiânia-GO., para detecção de rotavírus e adenovírus. Destas, 291 provinham de crianças diarréicas e 266 de não diarréicas. Das amostras não diarréicas, 64 eram provenientes de crianças de berçário. Das 557 amostras, 261 foram analisadas pela imunomicroscopia eletrônica (IME), eletroforese em gel de poliacrilamida (EGPA-SDS) e ensaio imunoenzimático para rotavírus e adenovírus (EIARA) e as demais apenas pela EGPA e EIARA. Rotavírus e adenovírus mostraram positividade de 17,2% e 2,1% respectivamente, e na condição de diarréia ou não, observou-se percentuais de 29,2% e 4,1% respectivamente para rotavírus (p<0,05) e 2,4% e 1,5% para adenovírus. Rotavírus foram mais prevalentes entre as crianças de 1-11 meses de idade e não foram vistos em nenhum recém-nato de berçário. Os adenovírus ocorreram na faixa de 1-3 anos. Rotavírus apresentaram maior circulação entre os meses de maio a agosto (p<0,05), não sendo encontrados de dezembro a fevereiro.<br>In order to detect rotavirus and adenovirus 557 feces samples from hospitalized children (0-5 years of age) were analysed from June 1987 to July 1990 in Goiânia-city.Two hundred and ninety one samples were from children with diarrhoea and 266 were from children without diarrhoea. Amongst this later group, 64 samples were from children from the nursery. Two hundred and sixty one out of 557 samples were analysed by immunoelectron microscopy (IEM), polyacrylamide gel electrophoresis (SDS-PAGE) and enzymatic immunoassay for rotavirus and adenovirus (EIARA) whereas the rest (296 samples) were analysed by SDS-PAGE and EIARA. Positivity of rotavirus and adenovirus was 17.2% and 2.1% respectively. Concerning rotavirus and adenovirus there was 29.2% and 2.4% positivity within the group with diarrhoea and 4.1% and 1.5% positivity amongst children without diarrhoea (p<0.05). Rotavirus were more prevalent amongst children which age ranged from 1 to 11 months of age. No newborn child from the nursery was positive for rotavirus. Adenovirus were detected amongst children from 1 to 3 years of age. Rotavirus circulation peak occurred between May and August (p<0.05) and no positive case was detected from December to February. Two hundred out of 291 diarrheic samples were also studied concerning bacteria and pathogenic parasites and equal percentages (17.0%) were found for both rotavirus and pathogenic bacteria. Eighty nine samples of rotavirus were detected by SDS-PAGE and 86 of these (96.6%) belonged to the subgroup II with 13 different electrophoretic patterns. Predominance of a given eletropherotic profile was observed in each year of the study