5 research outputs found
Láser y luz pulsada intensa en el tratamiento de hemangiomas infantiles y malformaciones vasculares
The use of the indications of the laser in treating
vascular malformations and infantile haemangiomas is
based on the theory of selective photothermolysis, in
which the oxyhaemoglobin is the target chromophore
on which the light of the laser acts, thus avoiding damage
to neighbouring tissues. The pulsed dye laser is the
most employed and at present is the treatment of
choice in capillary malformations (port-wine stains). A
variable response is obtained, with a substantial clearing
of the colour of the lesion after several sessions.
Application at early ages seems to improve the results.
Venous malformations, especially those localised in
the mucosa, respond better to the Nd:YAG laser; lymphatic
malformations to the CO2 laser. Arteriovenous
malformations rarely respond. Use of the pulsed dye
laser in the phase of proliferation of the haemangiomas
is subject to controversy, except where there is ulceration.
A rapid re-epithelialization is obtained in these
cases following its use. In the involution phase,
patients with residual vascular lesions can benefit from
other lasers such as KTP or Nd:YAG. If they show an
atrophic surface and scars these complications
improve with the CO2 laser or Er:YAG. New treatment
modalities are emerging, such as photodynamic therapy,
whose efficacy and safety, both in the treatment of
haemangiomas and vascular malformations, have yet
to be confirmed
Disseminated epidermolytic acanthoma probably related to trauma
Epidermolytic acanthoma is a rare benign tumour, which may occur in both isolated
and disseminated forms. Only seven cases of disseminated epidermolytic acanthoma
(DEA) have been described. This entity should be distinguished from other
hereditary or acquired conditions which involve epidermolytic hyperkeratosis and
other benign acanthomas. On the basis of the clinical history and the
histological findings, we diagnosed a case of DEA which was probably secondary to
repeated trauma
Cutaneous Biology: In vivo blockade of pemphigus vulgaris acantholysis by inhibition of intracellular signal transduction cascades
Pemphigus vulgaris (PV) is an autoimmune disease characterized by
mucocutaneous intraepithelial blisters and pathogenic autoantibodies against
desmoglein 3. The mechanism of blister formation in pemphigus has not been
defined; however, in vitro data suggest a role for activation of intracellular
signalling cascades. OBJECTIVES: To investigate the contribution of these
signalling pathways to the mechanism of PV IgG-induced acantholysis in vivo.
METHODS: We used the passive transfer mouse model. Mice were injected with IgG
fractions of sera from a patient with PV, with or without pretreatment with
inhibitors of proteins that mediate intracellular signalling cascades. RESULTS:
Inhibitors of tyrosine kinases, phospholipase C, calmodulin and the
serine/threonine kinase protein kinase C prevented PV IgG-induced acantholysis in
vivo. CONCLUSIONS: These observations strongly support the role of intracellular
signalling cascades in the molecular mechanism of PV IgG-induced acantholysi
Láser y luz pulsada intensa en el tratamiento de hemangiomas infantiles y malformaciones vasculares
The use of the indications of the laser in treating
vascular malformations and infantile haemangiomas is
based on the theory of selective photothermolysis, in
which the oxyhaemoglobin is the target chromophore
on which the light of the laser acts, thus avoiding damage
to neighbouring tissues. The pulsed dye laser is the
most employed and at present is the treatment of
choice in capillary malformations (port-wine stains). A
variable response is obtained, with a substantial clearing
of the colour of the lesion after several sessions.
Application at early ages seems to improve the results.
Venous malformations, especially those localised in
the mucosa, respond better to the Nd:YAG laser; lymphatic
malformations to the CO2 laser. Arteriovenous
malformations rarely respond. Use of the pulsed dye
laser in the phase of proliferation of the haemangiomas
is subject to controversy, except where there is ulceration.
A rapid re-epithelialization is obtained in these
cases following its use. In the involution phase,
patients with residual vascular lesions can benefit from
other lasers such as KTP or Nd:YAG. If they show an
atrophic surface and scars these complications
improve with the CO2 laser or Er:YAG. New treatment
modalities are emerging, such as photodynamic therapy,
whose efficacy and safety, both in the treatment of
haemangiomas and vascular malformations, have yet
to be confirmed
Pemphigus vulgaris autoantibodies induce apoptosis in HaCaT keratinocytes
Pemphigus vulgaris (PV) is an autoimmune disease characterized by binding of IgG autoantibodies to epidermal keratinocyte desmosomes. IgG autoantibodies obtained from a patient with mucocutaneous PV reacted with plakoglobin (Plkg) in addition to desmoglein-3 (Dsg3) and Dsg1. Immunofluorescence analysis confirmed that IgG autoantibodies, unlike antibodies from a healthy volunteer, caused disruption of cell-cell contacts in HaCaT keratinocytes. Moreover, apoptosis was enhanced in cells treated with autoantibodies compared to those treated with normal antibodies. The apoptotic process induced by IgG autoantibodies was characterized by caspase-3 activation, Bcl-2 depletion and Bax expression. The present report demonstrates that PV IgG autoantibodies promote apoptosis in HaCaT keratinocytes