RRS James Cook Cruise JC191 19 January - 1 March 2020 Hydrographic sections from the Florida Straits to the Canaries Current across 24ºN in the Atlantic Ocean

A hydrographic section across the North Atlantic Ocean at a nominal latitude of 24°N was occupied by the RRS James Cook (cruise identifier: JC191) from 19 January to 1 March, 2020. The ship departed from Port Everglades, USA, completing a total of 135 CTD stations over the Florida Straits, the western basin, Mid-Atlantic Ridge, eastern basin and eastern boundary up to Morocco, before ending the cruise in Santa Cruz de Tenerife, Spain. The main objectives of the JC191 research expedition was to collect/measure physical-, chemical-, and biological-ocean data with the purpose of estimating heat, freshwater and carbon budgets on low frequency time scales. All CTD stations had measurements from a CTD rosette equipped with temperature, conductivity, pressure, oxygen sensors, in addition to water captured from 24 niskin bottles fired at varying intervals throughout the full depth water column. The water from the niskin bottles was analysed for dissolved oxygen, carbon (DIC/TA), nutrients, and conductivity. Water for methane (CH4), C14, C13, and pigments (filtered) was collected for onshore analysis. The CTD rosette was also equipped with 2 RBR loggers measuring conductivity, temperature and pressure (up to 6,000m), and a lowered Acoustic Doppler Current Profiler (LADCP) making full depth velocity measurements. The 135 CTD stations include 2 carbon blank stations, and 2 bulk water stations for incubations. In addition to the CTD stations, the RRS James Cook has an underway system, which includes an intake for surface water to be pumped into the water bottle annex and the deck lab; two vessel mounted ADCPs (VMADCPs). A thermosalinograph and a fluorometer, installed in the water bottle annex, continually recorded conductivity, temperature and fluorescence. Water from the CTD was collected to calibrate the ship’s underway TSG. The VMADCPs, 75Hz and 150HZ, mounted on the drop keel record ocean velocities in roughly the top 300- and 600-m, respectively. Surface carbon and methane measurements were also recorded from the underway systems, and surface meteorological variables were monitored via the meteorological sampling system and the pumped water underway system. Finally bathymetric data were recorded an EA640 echosounder and a Kongsberg EM122 multibeam, both of which are mounted on the ship’s hull. Last, 5 Deep Apex Argo floats measuring conductivity, temperature, pressure and oxygen (except for one float not equipped with an optode) were deployed in the western basin. Many of the science party also engaged in extensive outreach via blogs and social media, heightening visibility of the science teams activities to the oceanographic community and the general public. This report summarises the data collected and analysed, and the methodology used for the acquisition and processing of the data onboard the James Cook during the JC191 research expedition

An investigation into the perspectives of providers and learners on MOOC accessibility

An effective open eLearning environment should consider the target learner’s abilities, learning goals, where learning takes place, and which specific device(s) the learner uses. MOOC platforms struggle to take these factors into account and typically are not accessible, inhibiting access to environments that are intended to be open to all. A series of research initiatives are described that are intended to benefit MOOC providers in achieving greater accessibility and disabled learners to improve their lifelong learning and re-skilling. In this paper, we first outline the rationale, the research questions, and the methodology. The research approach includes interviews, online surveys and a MOOC accessibility audit; we also include factors such the risk management of the research programme and ethical considerations when conducting research with vulnerable learners. Preliminary results are presented from interviews with providers and experts and from analysis of surveys of learners. Finally, we outline the future research opportunities. This paper is framed within the context of the Doctoral Consortium organised at the TEEM'17 conference

QoSVisor: QoS Framework for SDN

The increasing demand for network services and quality across wide selections of digital applications in the internet era has caused growing congestion and raised questions about how to deal with prioritizing data in ways tailored to particular uses of applications and managing peak congestion times. Software Defined Network (SDN) in particular Slicing Strategy, seems the best solution due to its new constitution intelligently implemented through the SDN OpenFlow protocol. However, Slicing Strategies specifically “FlowVisor” are limited in certain mechanisms such as Traffic Engineering (TE), which make it a requirement to find new ways to deliver Quality of Service (QoS) for different applications. In this paper, QoSVisor presented as an SDN extension action QoS Slicer based as an enhancement to the standard FlowVisor operation slicing tools to ensure the QoS for each Slice-based class of application

Interim outcomes of mechanical thrombectomy for deep vein thrombosis from the all-comer CLOUT registry

OBJECTIVES: The multicenter, prospective, single arm CLOUT registry assesses the safety and effectiveness of the ClotTriever System (Inari Medical, Irvine, CA) for the treatment of acute and nonacute lower extremity deep vein thrombosis (DVT) in all-comer patients. Reported here are the outcomes of the first 250 patients. METHODS: All-comer patients with lower extremity DVT were enrolled, including those with bilateral DVT, those with previously failed DVT treatment, and regardless of symptom duration. The primary effectiveness end point is complete or near-complete (≥75%) thrombus removal determined by independent core laboratory-adjudicated Marder scores. Safety outcomes include serious adverse events through 30 days and clinical outcomes include post-thrombotic syndrome severity, symptoms, pain, and quality of life through 6 months. RESULTS: The median age was 62 years and 40% of patients had contraindications to thrombolytics. A range of thrombus chronicity (33% acute, 35% subacute, 32% chronic) was observed. No patients received thrombolytics and 99.6% were treated in a single session. The median thrombectomy time was 28 minutes. The primary effectiveness end point was achieved in 86% of limbs. Through 30 days, one device-related serious adverse event occurred. At 6 months, 24% of patients had post-thrombotic syndrome. Significant and sustained improvements were observed in all clinical outcomes, including the Revised Venous Clinical Severity Score, the numeric pain rating scale, and the EuroQol Group 5-Dimension Self-Report Questionnaire. CONCLUSIONS: The 6-month outcomes from the all-comer CLOUT registry with a range of thrombus chronicities demonstrate favorable effectiveness, safety, and sustained clinical improvements

Functional expression of TcoAT1 reveals it to be a P1-type nucleoside transporter with no capacity for diminazene uptake

It has long been established that the Trypanosoma brucei TbAT1/P2 aminopurine transporter is involved in the uptake of diamidine and arsenical drugs including pentamidine, diminazene aceturate and melarsoprol. Accordingly, it was proposed that the closest Trypanosoma congolense paralogue, TcoAT1, might perform the same function in this parasite, and an apparent correlation between a Single Nucleotide Polymorphism (SNP) in that gene and diminazene tolerance was reported for the strains examined. Here, we report the functional cloning and expression of TcoAT1 and show that in fact it is the syntenic homologue of another T. brucei gene of the same Equilibrative Nucleoside Transporter (ENT) family: TbNT10. The T. congolense genome does not seem to contain a syntenic equivalent to TbAT1. Two TcoAT1 alleles, differentiated by three independent SNPs, were expressed in the T. brucei clone B48, a TbAT1-null strain that further lacks the High Affinity Pentamidine Transporter (HAPT1); TbAT1 was also expressed as a control. The TbAT1 and TcoAT1 transporters were functional and increased sensitivity to cytotoxic nucleoside analogues. However, only TbAT1 increased sensitivity to diamidines and to cymelarsan. Uptake of [3H]-diminazene was detectable only in the B48 cells expressing TbAT1 but not TcoAT1, whereas uptake of [3H]-inosine was increased by both TcoAT1 alleles but not by TbAT1. Uptake of [3H]-adenosine was increased by all three ENT genes. We conclude that TcoAT1 is a P1-type purine nucleoside transporter and the syntenic equivalent to the previously characterised TbNT10; it does not mediate diminazene uptake and is therefore unlikely to play a role in diminazene resistance in T. congolense

POLR1B and neural crest cell anomalies in Treacher Collins syndrome type 4

PURPOSE: Treacher Collins syndrome (TCS) is a rare autosomal dominant mandibulofacial dysostosis, with a prevalence of 0.2-1/10,000. Features include bilateral and symmetrical malar and mandibular hypoplasia and facial abnormalities due to abnormal neural crest cell (NCC) migration and differentiation. To date, three genes have been identified: TCOF1, POLR1C, and POLR1D. Despite a large number of patients with a molecular diagnosis, some remain without a known genetic anomaly. METHODS: We performed exome sequencing for four individuals with TCS but who were negative for pathogenic variants in the known causative genes. The effect of the pathogenic variants was investigated in zebrafish. RESULTS: We identified three novel pathogenic variants in POLR1B. Knockdown of polr1b in zebrafish induced an abnormal craniofacial phenotype mimicking TCS that was associated with altered ribosomal gene expression, massive p53-associated cellular apoptosis in the neuroepithelium, and reduced number of NCC derivatives. CONCLUSION: Pathogenic variants in the RNA polymerase I subunit POLR1B might induce massive p53-dependent apoptosis in a restricted neuroepithelium area, altering NCC migration and causing cranioskeletal malformations. We identify POLR1B as a new causative gene responsible for a novel TCS syndrome (TCS4) and establish a novel experimental model in zebrafish to study POLR1B-related TCS